Immunogenetics

, Volume 37, Issue 3, pp 193–198

Isolation of a novel cDNA clone showing marked similarity to ME491/CD63 superfamily

  • Nobuhiko Emi
  • Kenjiro Kitaori
  • Masao Seto
  • Ryuzo Ueda
  • Hidehiko Saito
  • Toshitada Takahashi
Original Articles

Abstract

A novel cDNA clone, A15, was isolated by the differential screening of a cDNA library of an immature T cell line, HPB-ALL using radioactive cDNA probes from the mRNA of either HPB-ALL or peripheral blood lymphocytes. It hybridized to a single mRNA species of about 2.0 kilobases which is expressed in HPB-ALL cell line, but not in the PBL or a promyelocytic leukemia cell line, HL-60. The A15 gene codes for a protein of 244 amino acids which contains four potential transmembrane domains and four possible N-linked glycosylation sites. A computer-aided comparison showed a marked similarity to several other membrane proteins: CD9, CD37, CD53, TAPA-1, Sm23, CO-029, and ME491/CD63.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Angelisova, P., Vicek, C., Stefanova, I., Lipoldova, M., and Horejsi, V.: The human leucocyte surface antigen CD53 is a protein structurally similar to the CD37 and MRC OX-44 antigens. Immunogenetics 32: 281–285, 1990Google Scholar
  2. Atkinson, B., Ernst, C. S., Ghrist, B. F. D., Ross, A. H., Clark, W. H., Herlyn, M., Herlyn, D., Maul, G., Stepleuski, Z., and Koprowski, H.: Monoclonal antibody to a highly glycosylated protein reacts in fixed tissue with melanoma and other tumors. Hybridoma 4: 243–255, 1985Google Scholar
  3. Azorsa, D. O., Hyman, J. A., and Hildreth, J. K.: CD63/Pltgp40:A platelet activation antigen identical to the stage-specific melanoma associated antigen ME491. Blood 178: 280–284, 1991Google Scholar
  4. Bazan, J. F.: Structural design and molecular evolution of a cytokine receptor superfamily. Proc Natl Acad Sci USA 87: 6938–6938, 1990Google Scholar
  5. Boucheix, C., Benoit, P., Frachet, P., Billard, M., Worthington, R. E., Gagnon, J., and Uzan, G.: Molecular cloning of the CD9 antigen. J Biol Chem 266: 117–122, 1991Google Scholar
  6. Classon, B. J., Williams, A. F., Willis, A. C., Seed, B., and Stamenkovic, I.: The primary structure of the human leukocyte antigen CD37, a species homologue to the rat MRC-44 antigen. J Exp Med 169: 1497–1502, 1989Google Scholar
  7. Emi, N., Ueda, R., and Takahashi, T.: Isolation of stable mouse L cell transfectant expressing human T cell differentiation antigen Tp40. J Immunol 134: 2817–2819, 1985Google Scholar
  8. Feinberg, A. P. and Vogelstein, B.: A technique for radio-labeling DNA restriction endonuclease fragments to high specific activity. Anal Biochem 132: 6–13, 1983Google Scholar
  9. Gaugitsch, H. W., Hofer, E., Huber, N. E., Sahnabl, E., and Baumruker, T.: A new superfamily of lymphoid and melanoma cell proteins with extensive homology to Schistosoma mansoni antigen Sm23. Eur J Immunol 21: 377–383, 1991Google Scholar
  10. Hotta, H., Ross, A. H., Huebner, K., Isobe, M., Wendeborn, S., Chao, M. W., Ricciardi, R. P., Tsujimoto, Y., Croce, C. M., and Koprowski, H.: Molecular cloning and characterization of an antigen associated with early stages of melanoma tumor progression. Cancer Res 48: 2955–2962, 1988Google Scholar
  11. Jansen, R., Kalousek, F., Fenton, W. A., Rosenberg, L. E., and Ledley, F. D.: Cloning of full-length methylmalonyl-CoA mutase from a cDNA library using the polymerase chain reaction. Genomics 4: 198–205, 1989Google Scholar
  12. Kozak, M.: The scanning model for translation: an update. J Cell Biol 108: 229–241, 1989Google Scholar
  13. Kyte, J. and Doolittle, R. F.: A simple method for displaying the hydropathic character of a protein. J Mol Biol 157: 105–132, 1982Google Scholar
  14. Lipman, D. J. and Pearson, W. R.: Rapid and sensitive protein similarity searches. Science 227: 1435–1441, 1985Google Scholar
  15. Maniatis, T., Fritsch, E. F., and Sambook, J.: Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratory, Cold Spring Harbor, 1982Google Scholar
  16. Metzelaar, M. J., Sixma, J. J., and Nieuwenhuis, H. K.: Detection of platelet activation using activation specific monoclonal antibodies. Blood Cells 16: 85–96, 1990Google Scholar
  17. Metzelaar, M. J., Wijingaard, P. J., Peters, P. J., Sixma, J. J., Nieuwenhuis, H. K., and Clevers, H. C.: CD63 antigen. J Biol Chem 266: 3239–3245, 1991Google Scholar
  18. Oren, R., Takahashi, S., Doss, C., Levy, R., and Levy, S.: TAPA-1, the target of an antiproliferative antibody, defines a new family of transmembrane proteins. Mol Cell Biol 10: 4007–4015, 1990Google Scholar
  19. Osada, H., Emi, N., Ueda, R., Seto, M., Koike, K., Suchi, T., Obata, Y., and Takahashi, T.: Genuine CD7 expression in acute leukemia and lymphoblastic lymphoma. Leuk Res 14: 869–871, 1990Google Scholar
  20. Rakowicz-Szulcynska, E. M. and Koprowski, H.: Nuclear uptake of monoclonal antibody to a surface glycoprotein and its effect on transcription. Arch Biochem Biophys 271: 366–379, 1989Google Scholar
  21. Sakaguchi, N., Berger, C. N., and Melchers, F.: Isolation of a cDNA copy of an RNA species expressed in murine pre-B cells. EMBO J 5: 2139–2147, 1986Google Scholar
  22. Schwartz-Albiez, R., Dorken, B., Hofmann, W., and Moldenhaer, G.: The B cell-associated CD37 antigen (gp40–52) structure and subcellular expression of an extensively glycosylatyed glycoprotein. J Immunol 140: 905–914, 1988Google Scholar
  23. Suzuki, H., Ueda, R., Obata, Y., Seto, M., Hirata, R., Iwamura, H., Ota, K., and Takahashi, T.: T-cell receptor β chain gene rearrangements in leukemias with immature T cell phenotype. Jpn J Cancer Res 77: 1069–1073, 1986Google Scholar
  24. Szala, S., Kasai, Y., Steplewski, Z., Rodeck, U., Koprowski, H., and Linnenbach, A. J.: Molecular cloning of cDNA for the human tumor-associated antigen CO-029 and identification of related transmembrane antigens. Proc Natl Acad Sci USA 87: 6833–6837, 1990Google Scholar
  25. Wright, M. D., Henkle, K. J., and Mitchell, G. F.: An immunogenic Mr. 23 000 integral membrane protein of Schistosoma mansoni worms that closely resembles a human tumor associated antigen. J Immunol 144: 3195–3200, 1990Google Scholar

Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Nobuhiko Emi
    • 1
  • Kenjiro Kitaori
    • 1
  • Masao Seto
    • 2
  • Ryuzo Ueda
    • 2
  • Hidehiko Saito
    • 1
  • Toshitada Takahashi
    • 2
  1. 1.First Department of Internal MedicineNagoya University School of MedicineNagoyaJapan
  2. 2.Aichi Cancer Center Research InstituteNagoyaJapan
  3. 3.First Department of Internal MedicineNagoya University School of MedicineNagoyaJapan

Personalised recommendations