Journal of Comparative Physiology A

, Volume 170, Issue 3, pp 373–378

Age-correlated changes and juvenile hormone III regulation of the syllable period specific responses of the L3 auditory interneurons in the cricket, Acheta domesticus

  • John Henley
  • Jodie Greenwood
  • John Stout
  • Gordon Atkins


  1. 1.

    L3 is an auditory interneuron in the prothoracic ganglion of the cricket, Acheta domesticus. The degree of syllable period (SP) specific decrement to model calling songs is age-specific in L3. In response to calling songs having 50 ms SPs, L3s in old females (23–28 days) exhibit less response decrement than those in young (4 days) females (Figs. 1, 2).

  2. 2.

    Two to 4 days after juvenile hormone III (JHIII) application to old females, L3s respond in a decrementing manner similar to those of young females. The changes in the SP selectivity of L3 by age and JHIII application, correlate well with changes that have been demonstrated to occur in the selectivity of phonotaxis under similar conditions (Fig. 3).

  3. 3.

    The threshold of L3 does not change with age, while changes in L3's decrement result from decreased excitation in old females in response to the first syllable of a chirp (Figs. 4, 5).

  4. 4.

    Injection of patterned current pulses (which reproduce the temporal pattern of the calling song) do not elicit decrement (Fig. 6).

  5. 5.

    Age-related changes in selectivity to SP of the calling song occurs above and below L3s threshold (Fig. 7).


Key words

Hormonal control Identified neurons Behavioral correlates Audition Orthoptera 



syllable period


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Atkins G, Pollack GS (1987a) Response properties of prothoracic, interganglionic sound-activated interneurons in the cricket Teleogryllus oceaniens. J Comp Physiol A 161: 681–693Google Scholar
  2. Atkins G, Pollack GS (1987b) Correlations between structure, topographic arrangement and spectral sensitivity of sound-sensitive interneurons in crickets. J Comp Neurol 266: 398–412Google Scholar
  3. Atkins G, Ligman S, Burghardt F, Stout JF (1984) Changes in phonotaxis by the female cricket Acheta domesticus L. after killing identified acoustic interneurons. J Comp Physiol A 154: 795–804Google Scholar
  4. Atkins G, Chiba A, Atkins S, Stout J (1988) Low-pass filtering of sound signals by a high frequency brain neuron and its input in the cricket Acheta domesticus L. J Comp Physiol A 164: 269–276Google Scholar
  5. Atkins S, Atkins G, Rhodes M, Stout JF (1989) Influence of syllable period on song encoding properties of an ascending auditory interneuron in the cricket Acheta domesticus. J Comp Physiol A 165: 827–836Google Scholar
  6. Atkins G, Henley J, Handysides R, Stout J (1992) Evaluation of the behavioral roles of ascending auditory interneurons in calling song phonotaxis by the female cricket (Acheta domesticus). J Comp Physiol A 170: 363–372Google Scholar
  7. Boyan GS (1980) Auditory neurons in the brain of a cricket Gryllus bimaculatus (De Geer). J Comp Physiol 140: 81–93Google Scholar
  8. Boyan GS (1981) Two-tone suppression of an identified auditory neurone in the brain of the cricket Gryllus bimaculatus (De Geer). J Comp Physiol 144: 117–12Google Scholar
  9. Doherty JA (1985) Trade-off phenomena in calling song recognition and phonotaxis in the cricket, Gryllus bimaculatus (Orthoptera Gryllidae). J Comp Physiol A 156: 787–801Google Scholar
  10. Fielden A (1960) Transmission through the last abdominal ganglion of the dragonfly nymph, Anax Imperator. J Comp Exp Biol 37: 832–844Google Scholar
  11. Furukawa N, Tomioka K, Yamaguchi T (1983) Functional anatomy of the musculature and innervation of the neck and thorax of the cricket, Gryllus bimaculatus. Zool Mag 92: 371–385Google Scholar
  12. Hayes V (1991) Expression of nicotinic acetylcholine receptors in the auditory neuron of crickets — a possible role in the regulation of phonotaxis. M.S. Thesis, Andrews University, MIGoogle Scholar
  13. Hennig RM (1988) Ascending auditory interneurons in the cricket Teleogryllus commodus (Walker): comparative physiology and direct connections with afferents. J Comp Physiol A 163: 135–143PubMedGoogle Scholar
  14. Koch PB, Hoffmann KH (1985) Juvenile hormone and reproduction in crickets. Gryllus bimaculatus DeGeer: corpus allatum activity (in vitro) in females during the adult life cycle. Physiol Entomol 10: 173–182Google Scholar
  15. Loher W, Schooley DA, Baker FC (1987) Influence of the ovaries on JH titer in Teleogryllus commodus. Insect Biochem 17: 1099–1102Google Scholar
  16. Moiseff A, Hoy R (1983) Sensitivity to ultrasound in an identified auditory neuron in the cricket: a possible link to phonotactic behavior. J Comp Physiol 152: 155–167Google Scholar
  17. Moiseff A, Pollack GS, Hoy RR (1978) Steering responses of flying crickets to sound and ultrasound: mate attraction and predator avoidance. Proc Natl Acad Sci USA 75: 4052–4056Google Scholar
  18. Pollack GS, Hoy R (1981) Phonotaxis to individual rhythmic components of a complex cricket calling song. J Comp Physiol 144: 367–373Google Scholar
  19. Popov AV, Markovich AM (1982) Auditory interneurons in the prothoracic ganglion of the cricket, Gryllus bimaculatus. II A high-frequency ascending neuron (HF1AN). J Comp Physiol 146: 351–359Google Scholar
  20. Popov AV, Shuvalov VF (1977) Phonotactic behavior of crickets. J Comp Physiol 119: 111–126Google Scholar
  21. Renucci M, Strambi C (1983) Juvenile hormone levels, vitellogenin and ovarian development in Acheta domesticus. Experientia 39: 618–620Google Scholar
  22. Sanes JR, Hildebrand JG (1976) Acetylcholine and its metabolic enzymes in developing antennal lobes of the moth Manduca sexta. Dev Biol 52: 105–120Google Scholar
  23. Sanes JR, Prescott DJ, Hildebrand JG (1977) Cholinergic neurochemical development of normal and deafferented antennal lobes during metamorphosis of the moth Manduca sexta. Dev Brain Res 119: 389–402Google Scholar
  24. Satelle DB (1980) Acetylcholine receptors of insects: Biochemical and physiological approaches. In: Ford MG, Usherwood PNR, Reay RC, Lunt GG (eds) Neuropharmacology and neurobiology, Ellis Horwood, Chichester, pp 445–497Google Scholar
  25. Schildberger K (1984) Temporal selectivity of identified auditory neurons in the cricket brain. J Comp Physiol A 155: 171–185Google Scholar
  26. Stewart WW (1978) Functional connections between cells as revealed by dye-coupling with a highly fluorescent naphthalimide tracer. Cell 14: 741–759Google Scholar
  27. Stewart WW (1981) Lucifer dyes — highly fluorescent dyes for biological tracing. Nature 292: 17–21Google Scholar
  28. Stout JF, McGhee RW (1988) Attractiveness of the male Acheta domesticus calling song to females. II. The relative importance of syllable period, intensity and chirp rate. J Comp Physiol A 164: 277–287Google Scholar
  29. Stout JF, DeHaan CH, McGhee RW (1983) Attractiveness of the male Acheta domesticus calling song to females. I. Dependence on each of the calling song features. J Comp Physiol 153: 509–521Google Scholar
  30. Stout JF, Burghardt F, Atkins G (1985) The characterization and possible importance for phonotaxis of ‘L’-shaped ascending acoustic interneurons in the cricket Acheta domesticus. In: Kalmring K, Elsner N (eds) Acoustic and vibrational communication in insects. Parey, Berlin Hamburg, pp 89–100Google Scholar
  31. Stout JF, DeHaan CH, Hall JC, Rhodes M (1988) Processing of calling songs by a L-shaped neuron in the prothoracic ganglion of the female cricket, Acheta domesticus. Physiol Entomol 13: 89–101Google Scholar
  32. Stout J, Atkins G, Zacharias D (1991) Regulation of cricket phonotaxis through hormonal control of the threshold of an identified auditory neuron. J Comp Physiol A 169: 765–772Google Scholar
  33. Thorson J, Weber T, Huber F (1982) Auditory behavior of the cricket. II. Simplicity of calling-song recognition in Gryllus, and anomalous phonotaxis at abnormal carrier frequencies. J Comp Physiol 146: 361–378Google Scholar
  34. Walikonis R, Schoun D, Zacharias D, Henley J, Coburn P, Stout J (1991) Attractiveness of the male Acheta domesticus calling song to females. III. The relation of age-correlated changes in syllable period recognition and phonotactic threshold to juvenile hormone III biosynthesis. J Comp Physiol A 169: 751–764Google Scholar
  35. Wennauer R, Kassel L, Hoffmann KH (1989) The effects of juvenile hormone, 20-hydroxyecdysone, precocene II, and ovariectomy on the activity of the corpora allata (in vitro) in adult female Gryllus bimaculatus. J Insect Physiol 35: 299–304Google Scholar
  36. Wohlers D, Huber F (1982) Processing of sound signals by six types of neurons in the prothoracic ganglion of the cricket, Gryllus campestris L. J Comp Physiol 146: 161–173Google Scholar
  37. Wohlers D, Huber F (1985) Topographical organization of the auditory pathway within the prothoracic ganglion of the cricket Gryllus campestris L. Cell Tissue Res 239: 555–56Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • John Henley
    • 1
  • Jodie Greenwood
    • 1
  • John Stout
    • 1
  • Gordon Atkins
    • 1
  1. 1.Department of BiologyAndrews UniversityBerrien SpringsUSA

Personalised recommendations