Biotechnology Letters

, Volume 5, Issue 1, pp 19–24 | Cite as

High productivity continuous ethanol fermentation with a flocculating mutant strain of Zymomonas mobilis

  • Jared E. Fein
  • Hugh G. Lawford
  • G. Ross Lawford
  • Bogdan C. Zawadzki
  • Robert C. Charley


High fermenter (volumetric) ethanol productivities (⩾80 g/lh−1) were attained in a simple single-stage continuous-stirred-tank-reactor (CSTR) employing a flocculent mutant of Zymomonas mobilis with a feed containing 100g/l glucose. Under these conditions a final ethanol concentration of 47.6 g/l was obtained, representing a maximum conversion efficiency of 97% of theoretical.


Glucose Fermentation Organic Chemistry Mutant Strain Conversion Efficiency 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


SR = Medium glucose concentration (g/l)


Biomass concentration (g/l)


Ethanol concentration (g/l)


Volumetric productivity (g ethanol/l/h)


Product yield coefficient (g ethanol/g glucose consumed)


Specific rate of ethanol formation (g ethanol/g cells/h)


Dilution rate (h−1)


Maximum dilution rate: ie., highest dilution rate at which the effluent glucose concentration ⩽ 4g/l (h−1)


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  1. Bland, R.R., Chen, H.C., Jewell, W.J., Bellamy, W.D., and Zall, R.R., (1982). Biotechnol. Letters, 4, 323–328Google Scholar
  2. de Boks, P.A., and van Eybergen, G.C., (1981). Biotechnol. Letters, 3, 577–582.Google Scholar
  3. Charley, R.C., Fein, J.E., Lavers, B.H., Lawford, H.G., and Lawford, G.R., (1982) — In preparation for submission to Biotechnology Letters.Google Scholar
  4. Fein, J.E., Zawadzki, B.C., Lawford, H.G., and Lawford, G.R., (1982A). — Submitted to Applied and Environmental Microbiology.Google Scholar
  5. Fein, J.E., Charley, R.C., Hopkins, K.A., Lavers, B., and Lawford, H.G., (1982B). — Submitted to Biotechnology Letters.Google Scholar
  6. Grote, W., Lee, K.J., and Rogers, P.L., (1980). Biotechnol. Letters, 2, 481–486.Google Scholar
  7. Lawford, G.R., Lavers, B.H., Good, D., Charley, R., Fein, J. and Lawford, H.G., (1982). Presented at The Royal Society of Canada International Symposium on Ethanol fron Biomass, October 13–15, 1982, Winnipeg, Canada.Google Scholar
  8. Lawford, H.G., Lavers, B.H., Fein, J.E., Charley, R.C., and Lawford, G.R., (1982) — In preparation for submission to Biotechnology Letters.Google Scholar
  9. Lee, J.H., Woodward, J.C., Pagan, R.J., and Rogers, P.L., (1981). Biotechnol. Letters, 3, 177–182.Google Scholar
  10. Lee, J.H., Skotnicki, M.L., and Rogers, P.J., (1982). Biotechnol. Letters, 4, 615–620.Google Scholar
  11. Margaritis, A., Bajpai, P.K., and Wallace, J.B., (1981). Biotechnol. Letters, 3, 613–618.Google Scholar
  12. Rogers, P.L., Lee, K.J., Skotnicki, M.L., and Tribe, D.E. (1982). In: “Advances in Biochemical Engineering”, Vol. 23, Ed. A. Feichter, Springer-Verlag (New York).Google Scholar
  13. Strandberg, G.W., Donaldson, T.L., and Arcuri, E.J., (1982). Biotechnol. Letters, 4, 347–352.Google Scholar

Copyright information

© Chapman & Hall 1983

Authors and Affiliations

  • Jared E. Fein
    • 1
  • Hugh G. Lawford
    • 2
    • 1
  • G. Ross Lawford
    • 2
  • Bogdan C. Zawadzki
    • 1
  • Robert C. Charley
    • 1
  1. 1.Weston Research CentreTorontoCanada
  2. 2.Department of BiochemistryUniversity of TorontoTorontoCanada

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