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Applied Microbiology and Biotechnology

, Volume 41, Issue 2, pp 250–256 | Cite as

Catechol ring-cleavage in Pseudomonas cepacia: the simultaneous induction of ortho and meta pathways

  • R. Y. Hamzah
  • B. S. Al-Baharna
Applied Microbial and Cell Physiology Original Paper

Abstract

This work demonstrates the ring-cleavage pathways of catechol on Pseudomonas cepacia ATCC 29351, formed upon its growth on salicylate and benzoate, each as a sole carbon source. When grown on salicylate, P. cepacia induces only the catechol ortho pathway by its induction of catechol 1,2-dioxygenase. However, interestingly, benzoate-grown cells induce the ortho and meta pathways for the biodegradation of catechol, by inducing simultaneously catechol 1,2-dioxygenase and 2,3-dioxygenase, respectively, in the ratio of 7:1. The results indicate that P. cepacia ATCC 29351 possesses the genetic capacity for enzymes of both the ortho- and meta-cleavage pathways of benzoate degradation, although the phenotypic expression for the ortho pathway is higher. The simultaneous induction of catechol 1,2- and 2,3-dioxygenase is not detected in salicylate degradation. Although catechol is the metabolic intermediate for both salicylate and benzoate, catechol did not induce either pathway when used as a sole carbon source.

Keywords

Enzyme Carbon Source Biodegradation Pseudomonas Catechol 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • R. Y. Hamzah
    • 1
  • B. S. Al-Baharna
    • 1
  1. 1.Biotechnology Program, College of Applied SciencesArabian Gulf UniversityManamaBahrain

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