Anatomy and Embryology

, Volume 186, Issue 5, pp 497–503 | Cite as

Enhancement of muscle regeneration in the rat gastrocnemius muscle by low energy laser irradiation

  • N. Weiss
  • U. Oron
Original Articles


The effect of low-energy laser (He-Ne) irradiation on the rate of skeletal muscle regeneration after partial excision of the rat gastrocnemius muscle was studied using quantitative histological morphometric methods. The injured zones of the experimental rats were subjected to direct He-Ne laser (632.8 nm wavelength) irradiation (6.0 mW for 2.3 min) immediately following injury and once daily thereafter for 5 days. Muscles that were injured as above and subjected to red or room light irradiation served as a control group. The volume fraction (percent of total volume of injured zone) of the mononucleated cells in the injured zone decreased gradually with time after injury, but more rapidly in the laser irradiated muscles than in the control. At 3 days post-injury the myotubes in the laser-irradiated muscles populated a significantly higher percentage (13.9 ± 1.1%) of the injured area than in the control muscles (7.8 ± 1.0%). The volume fraction of the young myofibers in the laser irradiated muscles exceeded 30.6 ± 2.2% and 49.6 ± 5.6% at 8 and 11 days post-injury, respectively, while in control muscles these structures comprised only 9.6 ± 1.0% and 27.2 ± 3.8% of the injured zone at 8 and 11 days after injury, respectively. It is concluded that He-Ne laser irradiation during the regeneration process promotes muscle maturation in the injured zone following partial excision of the rat gastro-cnemius muscle.

Key words

Skeletal muscle Regeneration Morphometry Histology Rat Laser 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Allbrook D (1981) Skeletal muscle regeneration. Muscle Nerve 4:234–245Google Scholar
  2. Assia E, Rosner M, Belkin M, Solomon A, Schwartz M (1989) Temporal parameters of low energy laser irradiation for optimal delay of post-traumatic degeneration of rat optic nerve. Brain Res 476:205–212Google Scholar
  3. Belkin M, Zaturunsky B, Schwartz M (1988) A critical review of low energy laser bioeffects. Lasers Light Ophthalmol 2:63–71Google Scholar
  4. Bischoff R (1986a) Proliferation of muscle satellite cells on intact myofibers in culture. Dev Biol 115:129–139Google Scholar
  5. Bischoff R (1986b) A satellite cell mitogen from crushed adult muscle. Dev Biol 115:140–147Google Scholar
  6. Bischoff R (1990) Cell cycle commitment of rat muscle satellite cells. J Cell Biol 111:201–207Google Scholar
  7. Carlson BM, Faulkner JA (1983) The regeneration of skeletal muscle fiber following injury. A review. Med Sci Sports Exerc 15:107–198Google Scholar
  8. Carrino DA, Oron U, Pechak DG, Caplan AI (1988) Reinitiation of chondroitin sulphate proteoglycan synthesis in regenerating skeletal muscle. Development 103:641–656Google Scholar
  9. Karu T (1989) Photobiology of low-power laser effects. Health Phys 56:691–704Google Scholar
  10. Kovacs IB, Mester E, Gorog P (1974) Stimulation of normal healing with laser beam in the rat. Experientia 30:1275–1280Google Scholar
  11. Loud AV, Anversa P (1984) Biology of disease: morphometric analysis of biological processes. Lab Invest 50:250–261Google Scholar
  12. Maltz L, Oron U (1990) Proteolytic enzyme activities during regeneration of the rat gastrocnemius muscle. J Neurol Sci 98:149–154Google Scholar
  13. Mathieu O, Cruz-Orive LM, Hoppeler H, Weibel ER (1981) Measuring error and sampling variation in stereology: comparison of the efficiency of various methods for planar image analysis. J Microsc 121:75–88Google Scholar
  14. Mester E, Spiry T, Sjende B (1973) Effect of low laser rays in wound healing. Bull Soc Int Chir 2:169–181Google Scholar
  15. Oron U (1984) Acetylcholinesterase and nerve axon formation during muscle regeneration in rats. Cell Mol Biol 30:411–416Google Scholar
  16. Oron U (1990) Proteolytic enzyme activity in rat hindlimb muscles in fetus and during post-natal development. Int J Dev Biol 34:457–460Google Scholar
  17. Passarella S, Casamassima E, Molinari S, Pastore D, Quagliariello E, Catalano IM, Cingolani A (1984) Increase of proton electrochemical potential and ATP synthesis in rat liver mitochondria irradiated in vitro by Helium-Neon laser. FEBS Lett 175:95–99Google Scholar
  18. Phillips GD, Knighton DR (1990) Angiogenic activity in damaged skeletal muscle. Proc Soc Exp Biol Med 193:197–202Google Scholar
  19. Plaghki L (1985) Regeneration et myogenese du muscle strié. J Physiol (Paris) 80:51–110Google Scholar
  20. Rochkind S, Barr Nea L, Bartal A, Nissan M, Lubart R, Razon N (1988) New methods of treatment of severely injured sciatic nerve and spinal cord. Acta Neurochir [Suppl] 43:91–93Google Scholar
  21. Roth D, Oron U (1985) Repair mechanisms involved in muscle regeneration following partial excision of the rat gastrocnemius muscle. Exp Cell Biol 53:107–114Google Scholar
  22. Schwartz M, Doron A, Erlich M, Lavie V, Benbasat S, Belkin M, Rochkind S (1987) Effect of low energy He-Ne laser irradiation on post-traumatic degeneration of adult rabbit optic nerve. Lasers Surg Med 7:51–55Google Scholar
  23. Sokal RR, Rohlf FG (1981) Biometry, 2nd edn. Freeman, LondonGoogle Scholar
  24. Weibel ER (1979) Stereological methods: practical methods for biological morphometry. Academic Press, LondonGoogle Scholar
  25. Weibel ER (1980) Stereological methods: theoretical foundations. Academic press, LondonGoogle Scholar
  26. Weiss N, Oron U (1991) Enhancement of repair process in gastrocnemius muscle in the rat by electromagnetic irradiation. Proceedings of 28th Meeting of Zoological Society of Israel, Tel Aviv, p 28Google Scholar
  27. White TP, Esser AK (1989) Satellite cell and growth factor involvement in skeletal muscle growth. Med Sci Sports Exerc 21:S158-S163Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • N. Weiss
    • 1
  • U. Oron
    • 1
  1. 1.Department of Zoology, The George S. Wise Faculty of Life SciencesTel Aviv UniversityRamat AvivIsrael

Personalised recommendations