Immunogenetics

, Volume 35, Issue 3, pp 183–189

A continuous restriction map from HLA-E to HLA-F. Structural comparison between different HLA-A haplotypes

  • Abdel El Kahloun
  • Corine Vernet
  • Anne-Marie Jouanolle
  • Joëlle Boretto
  • Valérie Mauvieux
  • Jean-Yves Le Gall
  • Véronique David
  • Pierre Pontarotti
Original articles

DOI: 10.1007/BF00185112

Cite this article as:
El Kahloun, A., Vernet, C., Jouanolle, AM. et al. Immunogenetics (1992) 35: 183. doi:10.1007/BF00185112

Abstract

The class I region of the human major histocompatibility complex contains genes encoding the classical transplantation antigens (HLA-A, B, and C), at least three new class I genes (HLA-E, F, and G) and many class I pseudogenes (including HLA-H). By pulse field gel electrophoresis and using five rare cutter enzymes, we have constructed a precise and continuous map of 1200 kilobases (kb) around HLA-A. The blots were hybridized with HLA-A, E, and F-specific probes and with new probes derived from yeast artificial chromosomes and cosmids of the class I region. We have compared the genomic organization of the same 1200 kb in three homozygous lymphoblastoid cell lines corresponding to three different HLA haplotypes (A3, A24, and A31). The differences in size observed may have been caused by insertions and deletions and may prove valuable in understanding the evolution of the HLA chromosomal region.

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Abdel El Kahloun
    • 1
  • Corine Vernet
    • 2
  • Anne-Marie Jouanolle
    • 1
  • Joëlle Boretto
    • 2
  • Valérie Mauvieux
    • 1
  • Jean-Yves Le Gall
    • 1
  • Véronique David
    • 1
  • Pierre Pontarotti
    • 2
  1. 1.Laboratoire de Biochimie Médicale B. Faculté de MédecineRennes CédexFrance
  2. 2.C.I.M.L.Marseille Cédex 9France
  3. 3.Centre Immunologie INSERM-CNRSMarseille Cedex 9France

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