Applied Microbiology and Biotechnology

, Volume 32, Issue 3, pp 269–273 | Cite as

l-Lysine production in continuous culture of an l-lysine hyperproducing mutant of Corynebacterium glutamicum

  • Toshihiko Hirao
  • Tetsuo Nakano
  • Tomoki Azuma
  • Masahiro Sugimoto
  • Toshihide Nakanishi
Article

Summary

l-Lysine production was studied in and volumetric productivity were 105 g/l and 5.6 g/l per hour, respectively. The latter corresponded to a 2.5-fold higher value than that of a fed-batch culture. Higher agitation or use of oxygen-enriched air was critical for high productivity. This indicates that oxygen supply greatly affects l-lysine productivity in continuous culture.

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References

  1. Akashi K, Ikeda S, Shibai H, Hirose Y (1979) Effect of oxygen supply on l-lysine, l-threonine and l-isoleucine fermentations. Agric Biol Chem 43:2087–2092Google Scholar
  2. Azuma T, Nakanishi T (1988) Factors affecting l-arginine production in the continuous culture of an l-arginine producer of Corynebacterium acetoacidophilum. J Ferment Technol 66:285–290Google Scholar
  3. Azuma T, Nakanishi T, Sugimoto M (1988) Isolation and characterization of a stable l-arginine producer from continuous culture broth of Corynebacterium acetoacidophilum. J Ferment Technol 66:279–284Google Scholar
  4. Becker MJ (1982) Product biosynthesis in continuous fermentation. Folia Microbiol 27:315–318Google Scholar
  5. Choi YJ, Tribe DE (1982) Continuous production of phenylalanine using an Esherichia coli regulatory mutant. Biotechnol Lett 4:223–228Google Scholar
  6. Michalski HJ, Krzystek L, Blaszczyk R, Jamroz T, Wieczorek A (1984) The effect of mean residence time and aeration intensity on the l-lysine production in a continuous culture. In: Third European Congress on Biotechnology, München, FRG, vol II. Verlag Chemie, Weinheim, pp 527–532Google Scholar
  7. Mimura A, Yoshida T, Matsuno T, Hashida W, Taguchi H, Otake T, Teramoto S (1963) Continuous fermentation of l-glutamic acid(II). Kinetic analysis of growth phase and its application to continuous fermentation (Part I). Hakko Kogaku Kaishi 41:275–281Google Scholar
  8. Park NH, Rogers PL (1986) l-Phenylalanine production in continuous culture using a hyperproducing mutant of Esherichia coli K-12. Chem Eng Commun 45:185–196Google Scholar
  9. Srinivasan VR, Summers RJ (1981) Continuous cultures of cells, vol 1, CRC Press Inc, Florida, p 97Google Scholar

Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Toshihiko Hirao
    • 1
  • Tetsuo Nakano
    • 1
  • Tomoki Azuma
    • 1
  • Masahiro Sugimoto
    • 1
  • Toshihide Nakanishi
    • 1
  1. 1.Technical Research LaboratoriesKyowa Hakko Kogyo Co., Ltd.YamaguchiJapan

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