Applied Microbiology and Biotechnology

, Volume 35, Issue 2, pp 231–232

(1,4)-β-d-Arabinoxylan arabinofuranohydrolase: a novel enzyme in the bioconversion of arabinoxylan

  • F. J. M. Kormelink
  • M. J. F. Searle-Van Leeuwen
  • T. M. Wood
  • A. G. J. Voragen
Short Contribution

Summary

An enzyme able to hydrolyse the terminal non-reducing α-l-arabinofuranoside residues from arabinoxylans only has been found. This enzyme was unable to split arabinofuranosyl linkages in a range of other arabinofuranosyl-containing substrates. Analysis of reaction mixtures of arabinoxylan with this enzyme did not show a shift in the molecular weight distribution of the arabinoxylan, even after 24 h of incubation. Only monomeric arabinose was released. 1H-Nuclear magnetic resonance studies of arabinoxylan treated with this enzyme, described as (1,4)-β-d-arabinoxylan arabinofuranohydrolase, indicated a specificity towards the single-substituted xylose in arabinoxylan.

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • F. J. M. Kormelink
    • 1
  • M. J. F. Searle-Van Leeuwen
    • 1
  • T. M. Wood
    • 2
  • A. G. J. Voragen
    • 1
  1. 1.Department of Food ScienceAgricultural UniversityWageningenThe Netherlands
  2. 2.The Rowett Research InstituteAberdeenUK

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