Applied Microbiology and Biotechnology

, Volume 36, Issue 5, pp 592–597

Glycerol conversion to 1,3-propanediol by newly isolated clostridia

  • Hanno Biebl
  • Sabine Marten
  • Hans Hippe
  • Wolf-Dieter Deckwer
Biotechnology

Summary

From pasteurized mud and soil samples glycerol-fermenting clostridia that produced 1,3-propanediol, butyrate and acetate were obtained. The isolates were taxonomically characterized and identified as Clostridium butyricum. The most active strain, SH1 = DSM 5431, was able to convert up to 110 g/l of glycerol to 56 g/l of 1,3-propanediol in 29 h. A few Clostridium strains from culture-collections (3 out of 16 of the C. butyricum group) and some isolates of Kutzner from cheese samples were also able to ferment glycerol, but the final concentration and the productivity of 1,3-propanediol was lower than in strain SH1. Strain SH1 grew well in a pH range between 6.0 and 7.5, with a weak optimum at 6.5, and was stimulated by sparging with N2. Best overall productivity was obtained in fed-batch culture with a starting concentration of 5% glycerol. In all fermentations the yield of 1,3-propanediol in relation to glycerol was higher than expected from NADH production by acid formation. On the other hand the H2 production was lower than expected, if per mole of acetyl coenzyme A one mole of H2 is released. The observations point to a substantial transfer of reducing potential from ferredoxin to NAD, which finally results in increased 1,3-propanediol production.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Biebl H (1991) Glycerol fermentation to 1,3-propanediol by Clostridium butyricum. Measurement of product inhibition by use of a pH-auxostat. Appl Microbiol Biotechnol 35:701–705Google Scholar
  2. Cato EP, George WL, Finegold SM (1986) Genus Clostridium. In: Sneath PHA, Mair NS, Scharpe ME, Holt JG (eds) Bergey's manual of systematic bacteriology, vol 2. Williams and Wilkins, Baltimore, pp 1141–1200Google Scholar
  3. Forage RG, Foster MA (1982) Glycerol fermentation in Klebsiella pneumoniae. Functions of the coenzyme B12-dependent glycerol and diol dehydratases. J Bacteriol 149:413–419Google Scholar
  4. Forsberg CW (1987) Production of 1,3-propanediol from glycerol by Clostridium acetobutylicum and other Clostridium species. Appl Environ Microbiol 53:639–643Google Scholar
  5. Günzel B (1991) Mikrobielle Herstellung von 1,3-Propanediol durch Clostridium butyricum und adsorptive Abtrennung von Diolen. Ph. D. thesis, University of Braunschweig, FRGGoogle Scholar
  6. Günzel B, Yonsel Ş, Deckwer W-D (1991) Fermentative production of 1,3-propanediol from glycerol by Clostridium butyricum up to a scale of 2 m3. Appl Microbiol Biotechnol 36:289–294Google Scholar
  7. Heyndrickx M, Vos P de, Vancanneyt M, Ley J de (1991) The fermentation of glycerol by Clostridium butyricum LMG 1212 t2 and 1213 t1 and C. pasteurianum LMG 3285. Appl Microbiol Biotechnol 34:637–642Google Scholar
  8. Holdeman LV, Moore WEC (1977) Anaerobe laboratory manual. Virginia Polytechnic Institute, Blackburg, Va.Google Scholar
  9. Homann T, Tag C, Biebl H, Deckwer W-D, Schink B (1990) Fermentation of glycerol to 1,3-propanediol by Klebsiella and Citrobacter strains. Appl Microbiol Biotechnol 33:121–126Google Scholar
  10. Kretschmann J, Caduck F-J, Deckwer W-D, Tag C, Biebl H (1989) Fermentative Herstellung von 1,3-Propanediol. German patent no. DE 3924423 A1Google Scholar
  11. Kutzner HJ (1963) Untersuchungen an Clostridien mit besonderer Berücksichtigung der für die Milchwirtschaft wichtigen Arten. Zentralbl Bakteriol Parasitenk Infektionskr Hyg Abt 1 Orig 191:441–450Google Scholar
  12. Mickelson MN, Werkman CH (1940) Formation of trimethylene glycol from glycerol by Aerobacter. Enzymologia 8:252–256Google Scholar
  13. Nakas JP, Schaedle M, Parkinson CM, Coonley CE, Tanenbaum SW (1983) System development for linked-fermentation production of solvents from algal biomass. Appl Environ Microbiol 46:1017–1023Google Scholar
  14. Petitdemange H, Cherrier C, Raval G Gay R (1976) Regulation of NADH and NADPH-ferredoxin oxidoreductases in clostridia of the butyric group. Biochim Biophys Acta 421:334–347Google Scholar
  15. Pfennig N (1978) Rhodocyclus purpureus gen. nov., sp. nov., a ring-shaped, vitamin B12-requiring member the family Rhodospirillaceae. Int J Syst Bacteriol 28:283–288Google Scholar
  16. Schink B, Zeikus JG (1982) Microbial ecology of pectin decomposition in anoxic lake sediments. J Gen Microbiol 128:393–404Google Scholar
  17. Schink B, Ward JC, Zeikus JG (1981) Microbiology of wet wood: importance of pectin degradation and Clostridium species in living trees. Appl Environ Microbiol 42:526–532Google Scholar
  18. Tag CG (1991) Mikrobielle Herstellung von 1,3-Propanedol. Ph. D. thesis, University of Oldenburg, FRGGoogle Scholar
  19. Takeda Y, Matsui T (1955) Mycological studies of acetone-butanol fermenting bacteria (KN-18)(Clostridium kainantoi n. sp.). Agric Biol Chem 29:78–82Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Hanno Biebl
    • 1
  • Sabine Marten
    • 1
  • Hans Hippe
    • 2
  • Wolf-Dieter Deckwer
    • 1
  1. 1.Gesellschaft für Biotechnologische Forschung mbHBraunschweigFederal Republic of Germany
  2. 2.Deutsche Sammlung von Mikroorganismen und Zellkulturen GmbHBraunschweigFederal Republic of Germany

Personalised recommendations