Plant Cell, Tissue and Organ Culture

, Volume 6, Issue 2, pp 115–125 | Cite as

Factors affecting plant regeneration from leaf segments of Anthurium scherzerianum Schott (Araceae) cultured in vitro

  • Thomas Geier


Regeneration from Anthurium scherzerianum leaf segments was found to be highly dependent on genotype and leaf age. Of the various medium factors tested, the NH4NO3 level had the most significant effect on callus and shoot formation from leaf tissue. A low level of NH4NO3 (200 mg/l) proved beneficial to the induction of regeneration in all genotypes investigated. As compared to induction, multiplication of callus and shoots in subcultures was less susceptible to the action of various medium factors. After isolation from callus, shoots readily formed roots in the absence of growth regulators. High NH4NO3 level (720 mg/l) strongly accelerated root formation. Rooting ability progressively decreased as a consequence of repeated shoot multiplication in the presence of benzyladenine (BA) as sole growth regulator. The advantages and limitations of different micropropagation schemes are discussed.

Key words

Anthurium scherzerianum micropropagation leaf explants regeneration ability callus multiplication shoot multiplication 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    D'Amato F (1978) Chromosome number variation in cultured cells and regenerated plants. In: Thorpe TA (ed) Frontiers of plant tissue culture 1978. Proc. 4th Intl Cong Plant Tissue and Cell Culture. Calgary. pp 287–295Google Scholar
  2. 2.
    Fersing G, Lutz A (1977) Étude comparative de la multiplication végétative in vitro deux espéces horticoles d'Anthurium: A. andreanum et A. scherzerianum. CR Acad SC Paris, Sèr D 284:2231–2233Google Scholar
  3. 3.
    Geier T (1982) Morphogenesis and plant regeneration from spadix fragments of Anthurium scherzerianum cultivated in vitro. In: Fujiwara A (ed) Plant tissue culture 1982. Proc 5th Intl Cong Plant Tissue and Cell Culture Tokyo. pp 137–138Google Scholar
  4. 4.
    Geier T (in press) Micropropagation of Anthurium scherzerianum: Propagation schemes and plant conformity. Acta Horticulturae.Google Scholar
  5. 5.
    Larkin PJ, Scowcroft WR (1981) Somaclonal variation — a novel source of variability from cell cultures for plant improvement. Theor Appl Genet 60:197–214Google Scholar
  6. 6.
    Leffring L, Hoogstrate J, Braster M (1976) Weefselkweek Anthurium: Onderzoek naar verbeteringsmethoden. Vakbl Bloem 31(9):17Google Scholar
  7. 7.
    Leffring L, Soede AC (1979) Weefselkweek Anthurium andreanum onderzoek to boven (1). Vakbl Bloem 34(13):43Google Scholar
  8. 8.
    Leffring L, Soede AC (1979) Weefselkweek Anthurium andreanum onderzoek to boven (2). Vakbl. Bloem 34(15):40–41Google Scholar
  9. 9.
    Meins FJr (1983) Heritable variation in plant cell culture. Ann Rev Plant Physiol 34:327–346Google Scholar
  10. 10.
    Murashige T, Skoog F (1962) A revised medium for rapid growth and bio assays with tobacco tissue cultures. Physiol Plant 15:473–497Google Scholar
  11. 11.
    Nitsch JP (1969) Experimental androgenesis in Nicotiana. Phytomorphology 19:389–404Google Scholar
  12. 12.
    Pierik RLM (1976) Anthurium andreanum plantlets produced from in vitro cultivated callus tissues. Physiol Plant 37:80–82Google Scholar
  13. 13.
    Pierik RLM, Steegmans HHM (1976) Vegetative Propagation of Anthurium scherzerianum Schott through callus cultures. Sci Hort 4:291–292Google Scholar
  14. 14.
    Pierik RLM, Steegmans HHM, Van der Meys JAJ (1974) Plantlet formation in callus tissues of Anthurium andreanum Lind. Sci Hort 2:193–198Google Scholar
  15. 15.
    Pierik RLM, Van Leeuwen P, Rigter GCCM (1979) Regeneration of leaf explants of Anthurium andreanum Lind. in vitro. Neth J agric Sci 27:221–226Google Scholar
  16. 16.
    Zens A, Zimmer K (in press) Untersuchungen zur in vitro Vermehrung von Anthurium scherzerianum. Gartenbauwissenschaft.Google Scholar

Copyright information

© Martinus Nijhoff Publishers 1986

Authors and Affiliations

  • Thomas Geier
    • 1
  1. 1.Institut für BotanikForschungsanstalt für Weinbau, Gartenbau, Getränketechnologie und LandespflegeGeisenheimGermany

Personalised recommendations