Applied Microbiology and Biotechnology

, Volume 34, Issue 4, pp 463–468 | Cite as

Production of 2,3-butanediol in a membrane bioreactor with cell recycle

  • A. -P. Zeng
  • H. Biebl
  • W. -D. Deckwer
Biochemical Engineering


The production of 2,3-butanediol by Enterobacter aerogenes DSM 30053 was studied in a cell recycle system with a microfiltration module. Emphasis was put on the influence of oxygen supply, cell residence time, dilution rate, and pH. Under optimal conditions a productivity as high as 14.6 g butanediol + acetoin/l per hour was achieved with a product concentration of 54 g/l and a product yield of 88%. This productivity is three times higher than that of an ordinary continuous culture. The achievable final product concentration of a cell recycle system was limited by the accumulation of the inhibiting by-product acetic acid, which increased very rapidly at low dilution rate. To maximize product concentration a fed-batch fermentation was carried out with stepwise pH adaption at high cell density. A final product concentration of 110 g/l was obtained with a productivity of 5.4 g/l per hour and a yield of 97%.


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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • A. -P. Zeng
    • 1
  • H. Biebl
    • 1
  • W. -D. Deckwer
    • 1
  1. 1.Biochemical Engineering DivisionGBF-National Research Institute for BiotechnologyBraunschweigGermany

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