Biotechnology Techniques

, Volume 10, Issue 12, pp 953–958 | Cite as

Quantitative measurement of green fluorescent protein expression

  • C. Renee Albano
  • Lisa Randers-Eichhon
  • Qing Chang
  • William E. Bentley
  • Govind Rao
Article

Summary

Data presented here shows a time course analysis of E. coli shake flask cultures expressing the reporter gene green fluorescent protein (GFP) with simultaneous comparison of microbial fluorescence intensity measurements and GFP concentration measured by Western blot. There is an apparent lag between the presence of GFP and its fluorescence due to the time required for formation of the chromophore. We demonstrate that GFP fluorescence can be used as a quantifiable reporter gene, provided the cyclization time for chromophore formation is considered.

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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • C. Renee Albano
    • 1
  • Lisa Randers-Eichhon
    • 2
  • Qing Chang
    • 2
  • William E. Bentley
    • 3
    • 4
  • Govind Rao
    • 1
    • 2
    • 5
  1. 1.Molecular and Cellular Biology ProgramUniversity of Maryland Baltimore CountyBaltimoreUSA
  2. 2.Department of Chemical and Biochemical EngineeringUniversity of Maryland Baltimore CountyBaltimoreUSA
  3. 3.Center for Agricultural BiotechnologyUniversity of Maryland Biotechnology InstituteUSA
  4. 4.Department of Chemical EngineeringUniversity of Maryland College ParkCollege ParkUSA
  5. 5.Medical Biotechnology Center of the Maryland Biotechnology InstituteUniversity of MarylandBaltimoreUSA

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