Biotechnology Letters

, Volume 15, Issue 8, pp 847–852

Characterization of a bacterial xylanase resistant to repression by glucose and xylose

  • Ranjana Srivastava
  • Alok K. Srivastava
Article

Summary

Bacillus subtilis CD4, when grown in nutrient broth or minimal medium in presence of xylan, produced extracellular xylanase that hydrolyzed xylan optimally at pH 5. The enzyme was induced by xylan, xylose and glucose. Addition of xylose or glucose in xylan containing medium did not affect enzyme production. The structural gene encoding xylanase was cloned and expressed in E. coli. The recombinant enzyme exhibited similar properties like that of native enzyme including resistance to repression by xylose and glucose.

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Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Ranjana Srivastava
    • 1
  • Alok K. Srivastava
    • 1
  1. 1.Division of Microbial GeneticsCentral Drug Research InstituteLucknowIndia

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