Biotechnology Letters

, Volume 15, Issue 8, pp 847–852

Characterization of a bacterial xylanase resistant to repression by glucose and xylose

  • Ranjana Srivastava
  • Alok K. Srivastava


Bacillus subtilis CD4, when grown in nutrient broth or minimal medium in presence of xylan, produced extracellular xylanase that hydrolyzed xylan optimally at pH 5. The enzyme was induced by xylan, xylose and glucose. Addition of xylose or glucose in xylan containing medium did not affect enzyme production. The structural gene encoding xylanase was cloned and expressed in E. coli. The recombinant enzyme exhibited similar properties like that of native enzyme including resistance to repression by xylose and glucose.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Berenger, J.F., Friscon, C., Gliarde, J.B. and Cruezer, N. (1985) Can. J. Microbiol. 31, 635–643.Google Scholar
  2. Bernier Jr., R., Driguez, H. and Desrochers, N. (1983) Gene 26, 59–65.Google Scholar
  3. Biely, P. (1985) Trends Biotechnol. 3, 286–290.Google Scholar
  4. Fisher, S.H. and Sonenshein, A.L. (1991) Annu. Rev. Microbiol. 45:107–35.Google Scholar
  5. Fisher, S.H. and Magasanik, B. (1984) J. Bacteriol. 158, 379–82.Google Scholar
  6. Gilbert, H.J., Sullivan, D.A., Jenkins, G., Kellett, L.E., Minton, N.P. and Hall, J. (1988) J. Gen. Microbiol, 134, 3239–3247.Google Scholar
  7. Klier, A.F. and Rapoport, G. (1988) Ann. Rev. Microbiol, 42:65–95.Google Scholar
  8. Maniatis, T., Fritsch, E.F. and Sambrook, J. (1982) Molecular cloning, a laboratory manual. Cold Spring Harbor Laboratory.Google Scholar
  9. Miller, G.L., Blum, R., Glennon, W.E. and Burton, A.L. (1960) Anal. Biochem. 2, 127–132.Google Scholar
  10. Miller, J.H. (1972) Experiments in Molecular genetics, CSHL, CSH, NY.Google Scholar
  11. Minton, N.P., Atkinson, T. and Sherwood, R.F. (1986) J. Bacteriol. 156, 1222–1227.PubMedGoogle Scholar
  12. Panbangred, W., Kondo, T., Negoro, S., Shinmyo, A. and Okada, H. (1983) Mol. Gen. Genet. 192, 335–341.Google Scholar
  13. Srivastava, R., Bharti, R. and Srivastava, A.K. (1990) Biotechnol. Lett. 12, 541–545.Google Scholar
  14. Srivastava, R., Ali, S.S. and Srivastava, B.S. (1991) FEMS Microbiol. Lett. 78 (2/3) 201–206.Google Scholar
  15. Wood, P.J., Erfle, J.D. and Teather, R.M. (1988) In Methods in Enzymology, 160, 63–70.Google Scholar
  16. Woodward, J. (1984) Xylanases: Functions, Properties and Applications. Top. Enzyme Ferment. Biotechnol. 8:9–30.Google Scholar
  17. Yang, R.C.A., McKenzie, C.R., Bilous, D., Seligy, V.L. and Narang, S.A. (1988) Appl. Environ. Microbiol. 54, 1023–1029.Google Scholar

Copyright information

© Chapman & Hall 1993

Authors and Affiliations

  • Ranjana Srivastava
    • 1
  • Alok K. Srivastava
    • 1
  1. 1.Division of Microbial GeneticsCentral Drug Research InstituteLucknowIndia

Personalised recommendations