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Naunyn-Schmiedeberg's Archives of Pharmacology

, Volume 343, Issue 4, pp 393–398 | Cite as

Effect of denervation on the neurogenic inflammation of the rat mandibular mucosa

  • Árpád Fazekas
  • Adrienne Györfi
  • Elek Pósch
  • Gábor Jakab
  • Zsolt Bártfai
  • László Rosivall
Article

Summary

Effects of local exposure to capsaicin on the vascular permeability and blood flow were studied in the rat oral mucosa at days 2 and 14 after the unilateral transcection of the inferior alveolar nerve (IAN). The distribution of nerve fibers displaying substance P (SP) and calcitonin gene-related peptide (CGRP) immunoreactivity (IR) in the mandibular mucosa was also assessed.

While the capsaicin-induced augmentation in vascular permeability was about 50% (P < 0.05) higher on the intact side (at both days 2 and 14) than on the denervated side, no difference in blood flow elevation was seen between the two sides. Transection of IAN caused only a slight reduction in the density of SP- and CGRP-IR fibers in the mucosa.

It is concluded that in addition to the IAN fibers the mandibular mucosa examined also seems to be supplied by other sensory fibers. The presence of accessory trigeminal branches was also supported by immunohistochemical studies.

Key words

Mandibular mucosa Local capsaicin irritation Neurogen inflammation Immunohistochemistry Denervation 

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References

  1. Aberdeen J, Corr L, Milner P, Lincoln J, Burnstock G (1990) Marked increases in calcitonin gene-related peptide-containing nerves in the developing rat following long-term sympathectomy with guanethidine. Neuroscience 35:175–184CrossRefGoogle Scholar
  2. Berger RL, Byers MR, Calkins DF (1983) Dental nerve regeneration in rats. I. Electrophysiological studies of molar sensory deficit and recovery. Pain 15:345–357CrossRefGoogle Scholar
  3. Brodin E, Gazelius B, Olgart L, Nilsson G (1981) Tissue concentration and release of substance P-like immunoreactivity in the dental pulp. Acta Physiol Scand 111:141–149CrossRefGoogle Scholar
  4. Christensen K (1940) Sympathetic nerve fibers in the alveolar nerves and nerves of the dental pulp. J Dent Res 19:227–242CrossRefGoogle Scholar
  5. Ekström, J, Ekman R, Håkanson R, Sjörgen S, Sundler F (1988) Calcitonin gene-related peptide in rat salivary glands: neuronal localization, depletion upon nerve stimulation and effects on salivation in relation to substance P. Neuroscience 26:933–949CrossRefGoogle Scholar
  6. Fazekas Á, Vindisch K, Pósch E, Györfi A (1990) Experimentally induced neurogenic inflammation in the rat oral mucosa. J Periodont Res 25:276–282CrossRefGoogle Scholar
  7. Fried K, Aldskogius H, Hildebrand C (1988) Proportion of unmyelinated axons in rat molar and incisor tooth pulps following neonatal capsaicin treatment and/or sympathectomy. Brain Res 463:118–123CrossRefGoogle Scholar
  8. Gamse R, Holzer P, Lembeck F (1980) Decrease of substance P in primary afferent neurons and impairment of neurogenic plasma extravasation by capsaicin. Br J Pharmacol 68:207–213CrossRefGoogle Scholar
  9. Handwerker HO, Holzer-Petsche U, Heym C, Welk E (1984) C-fibre functions after topical application of capsaicin to a peripheral nerve and after neonatal capsaicin treatment. In: Chahl L, Szolcsányi J, Lembeck F (eds) Antidromic vasodilation and neurogenic inflammation. Akadémiai Kiadó, Budapest, pp 57–78Google Scholar
  10. Holje L, Hildebrand C, Fried K (1983) Proportion of unmyelinated axons in the rat inferior alveolar nerve and mandibular molar pulps after neonatal administration of capsaicin. Brain Res 266:133–136CrossRefGoogle Scholar
  11. Holzer P (1988) Local effector functions of capsaicin-sensitive sensory nerve endings: involvement of tachykinins, calcitonin gene-related peptide and other neuropeptides. Neuroscience 24:739–768CrossRefGoogle Scholar
  12. Hsu SM, Raine L, Fauger H (1981) Use of avidin-biotin-peroxidase complex (ABC) in immunoperoxidase techniques. A comparison between ABC and unlabeled antibody (PAP) procedures. J Histochem Cytochem 29:577–580CrossRefGoogle Scholar
  13. Jancsó-Gábor, A. Szolcsányi J (1972) Neurogenic inflammatory responses. J Dent Res 51:264–269CrossRefGoogle Scholar
  14. Jancsó N, Jancsó-Gábor A, Szolcsányi J (1967) Direct evidence for neurogenic inflammation and its prevention by denervation and by pretreatment with capsaicin. Br J Pharmacol 31:138–151Google Scholar
  15. Jancsó G, Király E, Suck G, Joó F, Nagy A (1987) Neurotoxic effect of capsaicin in mammals. Acta Physiol Hung 69:295–313PubMedGoogle Scholar
  16. Lembeck F, Holzer P (1979) Substance P as neurogenic mediator of antidromic vasodilatation and neurogenic plasma extravasation. Naunyn-Schmiedeberg's Arch Pharmacol 310:175–183CrossRefGoogle Scholar
  17. Lundblad L, Saria A, Lundberg JM, Anggård A (1983) Increased vascular permeability in rat nasal mucosa induced by substance P and stimulation of capsaicin-sensitive trigeminal neurons. Acta Otolaryngol (Stockh) 96:479–484CrossRefGoogle Scholar
  18. Luthman J, Johansson O, Ahlström U, Kvint S (1988) Immunohistochemical studies of the neurochemical markers, CGRP, enkephalin, galamin, γ-MSH, NPY, PHI, proctolin, PTH, somatostatin, SP, VIP, tyrosine hydroxylase and beurofilament in nerves and cells of the human attached gingiva. Arch Oral Biol 33:149–158CrossRefGoogle Scholar
  19. Lynn B, Carpenter SE, Pini A (1984) Capsaicin and cutaneous afferents. In: Chahl L, Szolcsanyi J, Lembeck F (eds) Antidromic vasodilatation and neurogenic inflammation. Akadémiai Kiadó, Budapest, pp 83–92Google Scholar
  20. Merchenthaler J, Csernus V, Petruse P, Mess B (1988) New data on the immunocytochemical localization of thyreotropin-releasing hormone in the rat central nervous system. Am J Anat 181:359–376CrossRefGoogle Scholar
  21. Olgart L, Hökfelt T, Nilsson G, Pernow B (1977) Localization of substance P-like immunoreactivity in nerves in the tooth pulp. Pain 4:153–159CrossRefGoogle Scholar
  22. Robinson PP (1981) Reinnervation of teeth, mucous membrane and skin following section of the inferior alveolar nerve in the cat. Brain Res 220:241–253CrossRefGoogle Scholar
  23. Silverman JD, Kruger L (1989) Calcitonin gene-related-peptide-immunoreactive innervation of the rat head with emphasis on specialized sensory structures. J Comp Neurol 280:303–330CrossRefGoogle Scholar
  24. Sofromew MV, Schrell U (1982) Long-term storage and regular repeated use of diluted antisera in glass staining jars for increased sensitivity, reproducibility and convenience of single and two-colour light microscopic immunohistochemistry. J Histochem Cytochem 30:504–511CrossRefGoogle Scholar
  25. Soinila J, Salo A, Unsitalo H, Yanaihara N, Happole O (1989) CGRP-immunoreactive sensory nerve fibers in the submandibular gland of the rat. Histochemistry 91:455–460CrossRefGoogle Scholar
  26. Terenghi G, Zhang S-Q, Unger WG, Polak JM (1986) Morphological changes of sensory CGRP-immunoreactive and sympathetic nerves in peripheral tissues following chronic denervation. Histochemistry 86:89–95CrossRefGoogle Scholar
  27. Uddman R, Edvinsson L, Ekblad E, Håkanson R, Sundler F (1986a) Calcitonin gene-related peptide (CGRP): perivascular distribution and vasodilatory effects. Regul Pept 15:1–23CrossRefGoogle Scholar
  28. Uddman R, Gunditz T, Sundler F (1986b) Calcitonin gene related peptide: a sensory transmitter in dental pulps? Scand J Dent Res 94:219–224PubMedGoogle Scholar
  29. Wakisaka S, Nishikawa S, Ichukawa H, Matsuo S, Takano Y, Akai M (1985) The distribution and origin of substance P-like immunoreactivity in the rat molar pulp and periodontal tissues. Arch Oral Biol 30:813–818CrossRefGoogle Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Árpád Fazekas
    • 1
  • Adrienne Györfi
    • 2
  • Elek Pósch
    • 2
  • Gábor Jakab
    • 3
  • Zsolt Bártfai
    • 4
  • László Rosivall
    • 2
  1. 1.Department of Conservative DentistrySemmelweis University of MedicineBudapestHungary
  2. 2.Department of PathophysiologySemmelweis University of MedicineBudapestHungary
  3. 3.Department of NeurologySemmelweis University of MedicineBudapestHungary
  4. 4.II. Department of AnatomySemmelweis University of MedicineBudapestHungary

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