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Applied Microbiology and Biotechnology

, Volume 41, Issue 5, pp 584–590 | Cite as

Flow cytometry studies of recombinant Escherichia coli in batch and continuous cultures: DNA and RNA contents; light-scatter parameters

  • P. Fouchet
  • C. Manin
  • H. Richard
  • G. Frelat
  • J. N. Barbotin
Applied Genetics and Regulation Original Paper

Abstract

Flow cytometry has been used to study the contents of macromolecular compounds and light-scatter parameters in batch and continuous cultures of a recombinant Escherichia coli strain that forms protein inclusion bodies. Changes in relative DNA and RNA contents and cell mass as estimated by forward-angle light scatter were detected and tightly correlated in batch culture. In addition, heterogeneity of wide-angle light scatter (WALS), which we related to the presence of cellular inclusion bodies, was observed. In contrast, the relative RNA content and cell mass did not change during continuous culture, and homogeneity of WALS was found. In addition, unexpected changes in relative DNA content were observed after 67 h of culture, indicating a change in bacterial physiology.

Keywords

Cell Mass Inclusion Body Batch Culture Continuous Culture Light Scatter 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • P. Fouchet
    • 1
  • C. Manin
    • 2
  • H. Richard
    • 3
  • G. Frelat
    • 1
  • J. N. Barbotin
    • 2
  1. 1.Laboratoire de Cytométrie, Département de Pathologie et Toxicologie Expérimentales, Direction des Sciences du Vivant, Commissariat à l'Energie AtomiqueCentre d'Etudes Nucléaires de Fontenay aux RosesFontenay aux Roses CedexFrance
  2. 2.Laboratoire de Technologie Enzymatique (U.R.A. CNRS 1442)Université de Technologie de CompiègneCompiègne CedexFrance
  3. 3.Laboratoire de Cancérologie Expérimentale, Département de Pathologie et Toxicologie Expérimentales, Direction des Sciences du Vivant, Commissariat à l'Energie AtomiqueCentre d'Etudes Nucléaires de Fontenay aux RosesFontenay aux Roses CedexFrance

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