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Journal of Biomolecular NMR

, Volume 3, Issue 2, pp 233–237 | Cite as

A simple way for sequential assignment in isotopically enriched proteins using a H(N)CACO correlation

  • Stephan Seip
  • Jochen Balbach
  • Horst Kessler
Short Communications

Summary

A simplified scheme for sequential assignment in isotopically enriched proteins is presented. It is based on the standard triple resonance experiments HNCO, HN(CO)CA, HNCA and a modified H(N)CACO correlation, in which both of the HN connectivities to the CA/C′ pair of residue i and i-1 are observed. The H(N)CACO was tested on uniformly 13C/15N enriched P13 domain of mannose permease (31 kDa).

Keywords

Triple resonance Sequential assignment Isotope labeling Mannose permease Proteins 

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Copyright information

© ESCOM Science Publishers B.V. 1993

Authors and Affiliations

  • Stephan Seip
    • 1
  • Jochen Balbach
    • 1
  • Horst Kessler
    • 1
  1. 1.Organisch Chemisches InstitutTU MünchenGarchingGermany

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