Applied Microbiology and Biotechnology

, Volume 33, Issue 4, pp 469–472 | Cite as

Effects of lipids on thermophilic anaerobic digestion and reduction of lipid inhibition upon addition of bentonite

  • I. Angelidaki
  • S. P. Petersen
  • B. K. Ahring
Environmental Microbiology


The effect of bentonite-bound oil on thermophilic anaerobic digestion of cattle manure was investigated. In digestor experiments, addition of oil was found to be inhibitory during start-up and the inhibitory effect was less pronounced when the oil was added in the form of bentonite-bound oil compared to when the oil was added alone. After adaption of the digestors, very rapid degradation of oil was observed and more than 80% of the oil was degraded within a few hours after daily feeding. In batch experiments, glyceride trioleate was found to be inhibitory to thermophilic anaerobic digestion when the concentrations were higher than 2.0 g/l. However, addition of bentonite (a clay mineral) at concentrations of 0.15% and 0.45% was found to partly overcome this inhibition. Addition of calcium chloride in concentration of 3 mM (0.033% w/v) showed a similar positive effect on the utilization of oil, but the effect was lower than with bentonite.


Lipid Calcium Chloride Clay Manure 
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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • I. Angelidaki
    • 1
  • S. P. Petersen
    • 1
  • B. K. Ahring
    • 1
  1. 1.Department of BiotechnologyThe Technical University of DenmarkLyngbyDenmark

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