European Radiology

, Volume 2, Issue 5, pp 459–464 | Cite as

Localised in vivo 1H spectroscopy of human bone and soft tissue tumours

  • H. Bongers
  • F. Schick
  • M. Skalej
  • C. F. Hess
  • W. I. Jung
Original Articles Muskuloskeletal Radiology


Localised 1H in vivo magnetic resonance spectroscopy was applied to fibrous and bone tumours before and during cytostatic treatment and radiotheraphy. The results of 24 studies in 18 patients with malignant tumours of the leg or pelvis are presented including cases of sarcoma, malignant fibrous histiocytoma, multiple myeloma, malignant lymphoma and metastasis. A double spin echo localisation method with water suppression was implemented on a 1.5 Tesla whole body unit. Voxel size was (13 mm)3 or (20 mm)3. The most common resonances besides lipids (16/18) were those of choline (10/18) and creatine (5/18). Creatine was always decreased in comparison to choline and often absent from tumour spectra. Additional resonances with phase distorsions from J-coupling (chemical shift region 1.8–2.5 and 2.2–4.0 ppm) were recorded. In the presence of lipids, lactate remained undetectable because special editing techniques were not available. Significant spectral differences between different tumour types were not evident. In about 30% of the investigations the spectra contained only water and lipid signals. Follow-up studies in three patients during radio- and chemotheraphy showed a decrease in metabolites (choline, creatine, unassigned signals between 1.0 and 2.5 ppm) after weeks and months. The decrease in choline was most pronounced paralleled by an increase in lipid/choline ratios.

Key words

1H NMR spectroscopy Bone tumours Soft tissue tumours Magnetic resonance 


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. 1.
    Bottomley PA (1989) Human in vivo NMR spectroscopy in diagnostic medicine: clinical tool or research probe? Radiology 170: 1–15Google Scholar
  2. 2.
    Bruhn H, Frahm J, Gyngell M, et al (1989) Noninvasive differentiation of tumors with use of localized 1H NMR spectroscopy in vivo: initial experience in patients with cerebral tumors. Radiology 172: 541–548PubMedGoogle Scholar
  3. 3.
    Frahm J, Bruhn H, Gyngell ML, et al (1989) Localized high resolution proton NMR spectroscopy using stimulated echos: initial applications to human brain in vivo. Magn Reson Med 9: 79–93Google Scholar
  4. 4.
    Gill SS, Thomas DGT, Van Bruggen N, et al (1990) Proton MR Spectroscopy of intracranial tumors: in vivo and in vitro studies. JCAT 14: 497–504Google Scholar
  5. 5.
    Narayana PA, Jackson EF, Hazle JD, et al (1988) In vivo localized proton spectroscopic studies of human gastrocemius muscle. Magn Reson Med 8: 151–159Google Scholar
  6. 6.
    Bruhn H, Frahm J, Gyngell ML, et al (1991) Localized proton NMR spectroscopy using stimulated echoes: applications to human skeletal muscle in vivo. Magn Reson Med 17: 82–94Google Scholar
  7. 7.
    Barany M, Langer BG, Glick RPO, Venkatasubramanian PN, Wilbur AC, Spigos DG (1988) In vivo 1H spectroscopy in humans at 1.5 T. Radiology 167: 839–844Google Scholar
  8. 8.
    Jung WI, Lutz O (1989) Double spin echo volume selective NMR spectroscopy with a 1.5 T whole-body imager. Z. Naturforsch 44a: 1183–1186Google Scholar
  9. 9.
    Gordon RE, Ordidge RJ (1984) Volume selection for high resolution NMR studies. In: Book of abstracts: Society of Magnetic Resonance in Medicine, 3rd Annual Meeting, pp 272–273Google Scholar
  10. 10.
    Miller BL (1991) A review of chemical issues in 1H NMR spectroscopy: N-acetyl-L-aspartate, creatine and choline. NMR Biomed 4: 47–52Google Scholar
  11. 11.
    Semmler W, Gademann G, Bachert-Baumann P, et al (1988) Monitoring human tumor response to therapy by means of P-31 MR spectroscopy. Radiology 166: 533–539Google Scholar
  12. 12.
    Mountford CE, Delikatny EJ, Dyne M, et al (1990) Uterine cervical punch biopsy specimens can be analysed by 1H MRS. Magn Reson Med 13: 324–331Google Scholar
  13. 13.
    Mountford CE, May GL, Williams PG, et al (1986) Classification of human tumours by high resolution magnetic resonance spectroscopy. Lancet II: 651–653Google Scholar
  14. 14.
    Pan JW, Hamm JR, Hetherington HP, et al (1991) Correlation of lactate and ph in human skeletal muscle after exercise by 1H NMR. Magn Reson Med 20: 57–65Google Scholar
  15. 15.
    Bovée WMMJ (1991) Quantification of glutamate, glutamine, and other metabolites in in vivo proton NMR spectroscopy. NMR Biomed 4: 81–84Google Scholar
  16. 16.
    Zijl PCM van, Moonen CTW, Gillen J, et al (1987) Localized proton spectroscopy of superficial tumors in humans. Spectral changes upon radiation treatment of squamous cell carcinoma. In: Book of abstracts: Society of Magnetic Resonance in Medicine, 6th annual meeting, Vol.2, p 1005Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • H. Bongers
    • 1
  • F. Schick
    • 2
  • M. Skalej
    • 1
  • C. F. Hess
    • 3
  • W. I. Jung
    • 2
  1. 1.Department of Diagnostic RadiologyUniversity of TübingenTübingenFRG
  2. 2.Institute of PhysicsUniversity of TübingenTübingenFRG
  3. 3.Department of RadiotherapyUniversity of TübingenTübingenFRG

Personalised recommendations