European Archives of Oto-Rhino-Laryngology

, Volume 247, Issue 3, pp 149–150 | Cite as

Histological evaluation of cochlear blood flow using different fixation methods

  • Y. Kaseki
  • T. Nakashima
  • N. Yanagita


For an accurate histological evaluation of cochlear blood flow, it is essential to fix the cochlear vessels while maintaining their physiological state. In the present study, we administered 10% CO2 to guinea pigs and then used phase-contrast microscopy to determine how two different methods of fixation influenced the cochlear vasculature. The first method of fixation employed perilymphatic perfusion in vivo, while the second one involved fixation after decapitation. Decapitation caused significant changes in the vessels of the stria vascularis, including constriction and sludging. In contrast, no sludging occurred in the perilymphatic perfusion method and erythrocyte morphology was preserved. However, dilatation of the strial blood vessels occurred after the inhalation of 10% CO2 even in the decapitation method. The results demonstrate that particular attention must be paid to the fixation method used, especially when evaluating the blood flow of the stria vascularis.

Key words

Histology Cochlear blood flow Carbogen Perilymphatic perfusion Guinea pigs 


  1. 1.
    Axelsson A, Miller J, Larsson B (1975) A modified “soft surface specimen technique” for examination of the inner ear. Acta Otolaryngol (Stockh) 80:362–375Google Scholar
  2. 2.
    Dengerink HA, Axelsson A, Miller JM, Wright JW (1984) The effect of noise and carbogen on cochlear vasculature. Acta Otolaryngol (Stockh) 98:81–88Google Scholar
  3. 3.
    Dengerink HA, Axelsson A, Miller JM, Wright JW, Miller J (1987) Histological measures of cochlear blood flow. Acta Otolaryngol (Stockh) 104: 113–118Google Scholar
  4. 4.
    Miles FP, Nuttall NL (1988) In vivo capillary diameters in the stria vascularis and spiral ligament of the guinea pig cochlea. Hear Res 33:191–200Google Scholar
  5. 5.
    Vertles D, Axelsson A (1979) Methodological aspects of some inner ear vascular techniqes. Acta Otolaryngol (Stockh) 88: 328–334Google Scholar
  6. 6.
    Watanabe K (1986) Change in the capillary permeability of the stria vascularis by different methods of death and fixation. Ann Otol Rhinol Laryngol 95: 427–431Google Scholar

Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Y. Kaseki
    • 1
  • T. Nakashima
    • 1
  • N. Yanagita
    • 1
  1. 1.Department of OtorhinolaryngologyNagoya University School of MedicineShowa-ku, NagoyaJapan

Personalised recommendations