Journal of Biomolecular NMR

, Volume 4, Issue 2, pp 193–200 | Cite as

Measurement of four-bond HN-Hα J-couplings in staphylococcal nuclease

  • Geerten W. Vuister
  • Ad Bax
Research Paper


Quantitative J-correlation and triple-resonance ECOSY-type experiments are used to unambiguously establish the presence of four-bond sequential HN-Hα J-couplings in the protein staphylococcal nuclease. Substantially negative \({}^{\text{4}}{\text{J}}_{{\text{H}}^\alpha {\text{H}}^{\text{N}} } \) values, ranging from -0.8 to -2.3 Hz, are observed when the Ψ angle is near +120°, and the following ϕ angle near +60°. For other conformations, the four-bond HN-Hα J-couplings fall between -0.5 and +0.5 Hz.


Four-bond J-coupling ECOSY Quantitative J-correlation Staphylococcal nuclease 


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Copyright information

© ESCOM Science Publishers B.V 1994

Authors and Affiliations

  • Geerten W. Vuister
    • 1
  • Ad Bax
    • 1
  1. 1.Laboratory of Chemical Physics, National Institute of Diabetes and Digestive and Kidney DiseasesNational Institutes of HealthBethesdaU.S.A.

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