Journal of Biomolecular NMR

, Volume 3, Issue 5, pp 569–576 | Cite as

Application of nonlinear sampling schemes to COSY-type spectra

  • Peter Schmieder
  • Alan S. Stern
  • Gerhard Wagner
  • Jeffrey C. Hoch
Research Paper


Nonlinear sampling along the t1 dimension is applied to COSY-type spectra. The sine dependence of the time domain signals for the cross peaks is matched by a nonlinear sampling scheme that samples most densely around the maximum of the sine function. Data are processed by maximum entropy reconstruction, using a modified implementation of the ‘Cambridge’ algorithm of Skilling and Bryan. The procedure is demonstrated for P.E.COSY spectra recorded on a cyclic hexapeptide and on a 126-residue domain of the protein villin. The number of t1 values in the nonlinearly sampled experiments was reduced by a factor of four compared to linear sampling. The sensitivity and resolution of the resulting spectra are comparable to those achieved by conventional methods. The method described can thus significantly reduce the measuring time for COSY-type spectra.


Nonlinear sampling COSY-type spectra Maximum entropy reconstruction Resolution enhancement Sensitivity enhancement 



two-dimensional correlation spectroscopy


exclusive COSY


primitive E.COSY


time proportional phase incrementation


free induction decay


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Copyright information

© ESCOM Science Publishers B.V. 1993

Authors and Affiliations

  • Peter Schmieder
    • 1
  • Alan S. Stern
    • 2
  • Gerhard Wagner
    • 1
  • Jeffrey C. Hoch
    • 2
  1. 1.Department of Biological Chemistry and Molecular PharmacologyHarvard Medical SchoolBostonU.S.A.
  2. 2.Rowland Institute for ScienceCambridgeU.S.A.

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