Advertisement

Applied Microbiology and Biotechnology

, Volume 38, Issue 2, pp 194–197 | Cite as

Production and characterization of extracellular lipase from Calvatia gigantea

  • Paul Christakopoulos
  • Constantina Tzia
  • Dimitris Kekos
  • Basil J. Macris
Biotechnology Short Contribution

Summary

A number of factors affecting production of extracellular lipase by the edible fungus Calvatia gigantea were investigated. Consecutive optimization of carbon and nitrogen sources, initial pH of culture medium and growth temperature resulted in an increase in lipase activity of 87%. Under optimum conditions, activities as high as 22.4 units ml−1 of culture medium were obtained, competing favourably with most activities reported for other lipase hyperproducing microorganisms. The enzyme was optimally active at pH 7.0 and 30°C and had, at optimum pH, half-lives of 75.7 and 22.9 min at 45 and 55°C. Both high activity and kinetic characteristics of the enzyme make this process worthy of further investigation.

Keywords

Nitrogen Enzyme Lipase Optimum Condition High Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Alexopoulos CJ (1962) Introductory mycology. Wiley, New YorkGoogle Scholar
  2. Anderson MM, McCarty E (1972) Rapid and sensitive assay for free fatty acids using rhodamine 6G. Anal Biochem 45:260–270Google Scholar
  3. Baillargeon MW, Bistiine RG, Sonnet PE (1989) Evaluation of strains of Geotrichum candidum for lipase production and fatty acid specificity. Appl Microbiol Biotechnol 30:92–96Google Scholar
  4. Chander H, Batish VK, Sannabhadti SS, Srinivasan RA (1980) Factorsaffecting lipase production in Aspergillus wentii. J Food Sci 45:598–600Google Scholar
  5. Chander H, Klostermeyer H (1983) Production of lipase by Geotrichum candidum under various growth conditions. Milchwissenschaft 38:410–412Google Scholar
  6. Chopra AK, Chander H (1983) Factors affecting lipase production in Syncephalastrum racemosum. J Appl Bacteriol 54:163–169Google Scholar
  7. Eitenmiller RR, Vakil JR, Shahani KM (1970) Production and properties of Penicillium roqueforti lipase. J Food Sci 35:130–133Google Scholar
  8. Espinosa E, Sanchez S, Farres A (1990) Nutritional factors affecting lipase production by Rhizopus delemar CDBB H313. Biotechnol Lett 12:209–214Google Scholar
  9. Fischer B, Kleber HP (1987) Isolation and characterization of the extracellular lipase of Acinetobacter calcoacetigus 69 V. J Basic Microbiol 27:342–427Google Scholar
  10. Galiotou-Panayotou M, Macris BJ (1986) Degradation of condensed tannins by Calvatia gigantea. Appl Microbiol Biotechnol 23:502–506Google Scholar
  11. Hegedus DD, Khachatourians GG (1988) Production of extracellular lipase by Beauveria bassiana. Biotechnol Lett 10:637–642Google Scholar
  12. Ibrahim CO, Hayas BJ, Jhi M, Nagal S (1987) Purification and some properties of a thermostable lipase from Humicola lanuginosa no.3. Agric Biol Chem 51:37–45Google Scholar
  13. Iwai M, Okumura S, Tsujisaka Y (1980) Synthesis of terpene alcohol esters by lipase. Agric Biol Chem 44:2731–2732Google Scholar
  14. Kekos D, Macris BJ (1983a) Production of microbial protein from the edible fungus Calvatia gigantea grown on carbon sources containing high amounts of tannins. Proceedings of the VI World Congress Food Science and Technology, Dublin, Ireland, 18–23 September, vol 2: pp 165–166Google Scholar
  15. Kekos D, Macris BJ (1983b) Production and characterization of amylase from Calvatia gigantea. Appl Environ Microbiol 45:935–941Google Scholar
  16. Kekos D, Macris BJ (1986) Effect of tannins on growth and amylase production by Calvatia gigantea. Enzyme Microb Technol 9:94–96Google Scholar
  17. Kekos D, Galiotou-Panayotou M, Macris BJ (1987) Some nutritional factors affecting α-amylase production by Calvatia gigantea. Appl Microbiol Biotechnol 26:527–530Google Scholar
  18. Langrand G, Rondot N, Triantaphylides C, Baratti J (1990) Short chain flavour ester synthesis by microbial lipases. Biotechnol Lett 12:581–586Google Scholar
  19. Macrae AR, Hammond RC (1985) Present and future applications of lipases. Biotechnol Genet Eng Rev 3Google Scholar
  20. Muderhwa JM, Ratomahenina R, Graille J, Galzy P (1985) Purification and properties of the lipase from Candida deformans (Zach) Langeron and Guerra. J Am Oil Chem Soc 62:1031–1036Google Scholar
  21. Muraoka T, Ando T, Okuda H (1982) Purification and properties of a novel lipase from Staphylococcus aureus 226. J Biochem 92:1933–1939Google Scholar
  22. Nahas E (1988) Control of lipase production by Rhizopus oligosporus under various growth conditions. J Gen Microbiol 134:227–233Google Scholar
  23. Nair CS, Bone DH (1987) Production of lipase of Aspergillus foetidus in a batch stirred reactor. Biotechnol Lett 9:601–604Google Scholar
  24. Nakashima T, Fukuda H, Kyotani S, Morikawa H (1988) Culture conditions for intreacellular lipase production by Rhizopus chinensis and its immobilization within biomass support particles. J Ferment Technol 66:441–446Google Scholar
  25. Nishio T, Takahashi K, Yoshimoto T, Koders Y, Saito Y, Inada Y (1987) Terpene alcohol synthesis by polyethylene glycol-modified lipase in benzene. Biotechnol Lett 9:187–190Google Scholar
  26. Oi S, Sawada A, Satomura Y (1969) Purification and some properties of two types of Penicillium lipase I and II, and conversion of types I and II under various modification conditions. Agric Biol Chem 31:1357–1366Google Scholar
  27. Petrovic SE, Skrinjar M, Becarevic A, Vujicic IF, Banka L (1990) Effect of various carbon sources on microbial lipases biosynthesis. Biotechnol Lett 12:299–304Google Scholar
  28. Pulley JE (1969) Enzyme simplify processing. Food Eng 41:68–71Google Scholar
  29. Seitz EW (1974) Industrial application of microbial lipases: a review. J Am Oil Chem Soc 51:12–16Google Scholar
  30. Stern AM, Ordal ZJ, Halvorson HO (1954) Utilization of fatty acids by and lipolytic activities of Mucor mucedo. J Bacteriol 68:24–27Google Scholar
  31. Sugiura M, Oikawa T, Hirano K, Inukai T (1977) Purification crystallization and properties of triacyglycerol lipase from Pseudomonas fluorescens. Biochem Biophys Acta 488:353–358Google Scholar
  32. Suzuki M, Yamamoto H, Mizugaki M (1986) Purification and general properties of a metal-insensitive lipase from Rhizopus japonicus. J Biochem 100:1207–1213Google Scholar
  33. Sztajer H, Maliszewska I (1989) The effect of culture conditions on lipolytic productivity of Penicillium citrinum. Biotechnol Lett 11:895–898Google Scholar
  34. Tsujisaka Y, Iwai M, Tominaga Y (1973) Purification crystallization and some properties of lipase from Geotrichum candidum Link. Agric Biol Chem 37:1457–1464Google Scholar
  35. Tyski S, Hryniewicz W, Jeljaszewicz J (1983) Purification and some properties of the Staphylococcal extracellular lipase. Biotchem Biophys Acta 749:312–317Google Scholar
  36. Valero F, Ayats F, Lopez-Santin J, Poch M (1988) Lipase production by Candida rugosa: fermentation behaviour. Biotechnol Lett 10:741–744Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Paul Christakopoulos
    • 1
  • Constantina Tzia
    • 1
  • Dimitris Kekos
    • 1
  • Basil J. Macris
    • 1
  1. 1.Department of Chemical EngineeringNational Technical University of AthensZografou Campus AthensGreece

Personalised recommendations