Applied Microbiology and Biotechnology

, Volume 38, Issue 2, pp 194–197 | Cite as

Production and characterization of extracellular lipase from Calvatia gigantea

  • Paul Christakopoulos
  • Constantina Tzia
  • Dimitris Kekos
  • Basil J. Macris
Biotechnology Short Contribution


A number of factors affecting production of extracellular lipase by the edible fungus Calvatia gigantea were investigated. Consecutive optimization of carbon and nitrogen sources, initial pH of culture medium and growth temperature resulted in an increase in lipase activity of 87%. Under optimum conditions, activities as high as 22.4 units ml−1 of culture medium were obtained, competing favourably with most activities reported for other lipase hyperproducing microorganisms. The enzyme was optimally active at pH 7.0 and 30°C and had, at optimum pH, half-lives of 75.7 and 22.9 min at 45 and 55°C. Both high activity and kinetic characteristics of the enzyme make this process worthy of further investigation.


Nitrogen Enzyme Lipase Optimum Condition High Activity 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Paul Christakopoulos
    • 1
  • Constantina Tzia
    • 1
  • Dimitris Kekos
    • 1
  • Basil J. Macris
    • 1
  1. 1.Department of Chemical EngineeringNational Technical University of AthensZografou Campus AthensGreece

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