Biotechnology Techniques

, Volume 10, Issue 6, pp 401–406 | Cite as

Silkworm hemolymph as a substitute for fetal bovine serum in insect cell culture

  • Sung Ho Ha
  • Tai Hyun Park
  • Sam-Eun Kim


The effectiveness of silkworm hemolymph was investigated as a substitute for fetal bovine serum (FBS) in insect cell culture. Cells were adapted to grow in reduced FBS medium supplemented with silkworm hemolymph through a gradual adaptation process. FBS concentration in the medium could be reduced to 1% without decrease in cell growth rate and maximum cell concentration by adding 5% silkworm hemolymph.


Growth Rate Cell Culture Cell Growth Bovine Serum Fetal Bovine Serum 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Grace, T.D.C. (1962). Nature 195, 788–789.Google Scholar
  2. Ham, R.G. and McKeehan, W.L. (1979). Media and growth requirements. In: Methods in Enzymology, W.B. Jakoby and I.H. Pastan, eds. vol. 58. pp. 44–93, New York: Academic Press.Google Scholar
  3. Martignoni, M.E. and Scallion, R.J. (1961). Biol. Bull. 121, 507–520.Google Scholar
  4. Mitsuhashi, J. and Maramorosch, K. (1964). Contrib. Boyce Thompson Inst. 22, 435–460.Google Scholar
  5. Murhammer, D.W. and Goochee, C.F. (1988). Bio/Technol. 6. 1411–1418.Google Scholar
  6. Summers, M.D. and Smith, G.E. (1987). A mannual method for baculovirus vectors and insect cell culture procedures, Texas Agricultural Experiment Station Bulletin No. 1555, Texas A&M University, College Station, Texas.Google Scholar
  7. Wyatt, G.R., Loughheed, T.C., and Wyatt, S.S. (1956). J. Gen. Physiol. 39, 853–868.Google Scholar
  8. Wyatt, S.S. (1956). J. Gen. Physiol. 39, 841–852.Google Scholar
  9. Zhang, J, Kalogerakis, N., Behie, L., and Iatrou, K. (1992). Biotechnol. Bioeng. 40, 1165–1172.Google Scholar

Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Sung Ho Ha
    • 1
  • Tai Hyun Park
    • 1
  • Sam-Eun Kim
    • 2
  1. 1.Department of Genetic EngineeringSung Kyun Kwan UniversitySuwonKorea
  2. 2.Division of GeneticsSericulture & Insect Research InstituteSuwonKorea

Personalised recommendations