Biotechnology Techniques

, Volume 10, Issue 5, pp 335–338 | Cite as

Preparation of optically pure chiral amines by lipase-catalyzed enantioselective hydrolysis of N-acyl-amines

  • Hauke Smidt
  • Andreas Fischer
  • Peter Fischer
  • Rolf D. Schmid


A new and effective method using lipase from Candida antarctica (native or Novozym SP-435) for the preparation of enantiopure primary R-amines is reported. Hydrolysis of rac-N-acetyl-amines resulted in high conversion > 40% (168 hrs) and > 48% (> 240 hrs) and enantiomeric excesses (> 99% ee) for both the product and the remaining substrate.


Hydrolysis Lipase Candida High Conversion Enantiomeric Excess 
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Copyright information

© Chapman & Hall 1996

Authors and Affiliations

  • Hauke Smidt
    • 1
  • Andreas Fischer
    • 1
  • Peter Fischer
    • 2
  • Rolf D. Schmid
    • 1
  1. 1.Institute of Technical Biochemistry, University of StuttgartStuttgartGermany
  2. 2.Institute of Organic Chemistry and Isotope Research, University of StuttgartStuttgartGermany

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