Journal of Molecular Evolution

, Volume 41, Issue 6, pp 741–748 | Cite as

Phylogenetic relationships between tuna species of the genus Thunnus (Scombridae: Teleostei): Inconsistent implications from morphology, nuclear and mitochondrial genomes

  • Seinen Chow
  • Hirohisa Kishino
Articles

Abstract

In order to infer phylogenetic relationships between tuna species of the genus Thunnus, partial sequences of the mitochondrial cytochrome b and ATPase genes were determined in all eight species. Supplemental restriction analysis on the nuclear rRNA gene was also carried out. Pacific northern bluefin tuna (Thunnus thynnus orientalis) was found to have mtDNA distinct from that of the Atlantic subspecies (T. t. thynnus) but very similar to that from the species albacore (T. alaluga). In contrast, no differentiation in nuclear genome was observed between the Atlantic and Pacific northern bluefin tunas. The Atlantic northern bluefin and southern bluefin tunas possessed mtDNA sequences very similar to species of yellowfin tuna group and not so similar to albacore and bigeye tunas which were morphologically assigned to the bluefin tuna group. The molecular data indicate that (1) mtDNA from albacore has been incorporated into the Pacific population of northern bluefin tuna and has extensively displaced the original mtDNA, and (2) albacore is the earliest offshoot, followed by bigeye tuna in this genus, which is inconsistent with the phylogenetic relationships between these tuna species inferred from morphology.

Key words

Tuna Mitochondrial DNA transfer Phylogenetic analysis 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Adachi J, Hasegawa M (1994) MOLPHY: Programs for molecular phylogenetics, version 2.1.2. Institute of Statistical Mathematics TokyoGoogle Scholar
  2. Avise JC, Saunders NC (1984) Hybridization and introgression among species of sunfish (Lepomis): analysis by mitochondrial DNA and allozyme markers. Genetics 108:237–255Google Scholar
  3. Bartlett SE, Davidson WS (1991) Identification of Thunnus tuna species by the polymerase chain reaction and direct sequence analysis of their mitochondrial cytochrome b genes. Can J Fish Aquat Sci 48:309–317Google Scholar
  4. Block BA, Finnerty JR, Stewart AFR, Kidd J (1993) Evolution of endothermy in fish: mapping physiological traits on a molecular phylogeny. Science 260:210–214Google Scholar
  5. Brown WM, Prager EM, Wang A, Wilson AC (1982) Mitochondrial DNA sequences of primates: tempo and mode of evolution. J Mol Evol 18:225–239Google Scholar
  6. Campton DE (1987) Natural hybridization and introgression in fishes: methods of detection and genetic interpretations. In: Ryman N, Utter FU (eds) Population genetics and fishery management. University of Washington Press, Seattle, pp 161–192Google Scholar
  7. Chow S, Clarke ME, Walsh PJ (1993) PCR-RFLP analysis on thirteen western Atlantic snappers (subfamily Lutjaninae): a simple method for species and stock identification. Fish Bull 91:619–627Google Scholar
  8. Chow S, Inoue S (1993) Intra- and interspecific restriction fragment length polymorphism in mitochondrial genes of Thunnus tuna species. Bull Natl Res Inst Far Seas Fish 30:207–225Google Scholar
  9. Clark CG, Tague BW, Ware VC, Gerbi SA (1984) Xenopus laevis 28S ribosomal RNA: a secondary structure model and its evolutionary and functional implications. Nucleic Acids Res 12:6197–6220Google Scholar
  10. Collette BB (1978) Adaptation and systematics of the mackerels and tunas. In: Sharp GD, Dizon AE (eds) The physiological ecology of tunas. Academic Press, New York, pp 7–39Google Scholar
  11. Collette BB, Nauen CE (1983) FAO species catalogue. Scombrid of the world. FAO, RomeGoogle Scholar
  12. Elliott NG, Ward RD (1995) Genetic relationships of eight species of Pacific tunas (Teleostei, Scombridae) inferred from allozyme analysis. Mar Freshwat Res 46:(in press)Google Scholar
  13. Felsenstein J (1994) PHYLIP (phylogeny inference package). Department of Genetics, University of Washington, SeattleGoogle Scholar
  14. Ferris SD, Sage RD, Huang CM, Nielsen JT, Ritte U, Wilson AC (1983) Flow of mitochondrial DNA across a species boundary. Proc Natl Acad Sci USA 80:2290–2294Google Scholar
  15. Finnerty JR, Block BA (1995) Evolution of cytochrome b in the Scombroidei (Teleostei): molecular insights into billfish (Istiophoridae and Xiphiidae) relationships. Fish Bull 93:78–96Google Scholar
  16. Gibbs RHJ, Collette BB (1967) Comparative anatomy and systematics of the tunas, genus Thunnus. Fish Wildl Ser Fish Bull 66:65–130Google Scholar
  17. Hasegawa M, Kishino H, Yano T (1985) Dating of the human-ape splitting by a molecular clock of mitochondrial DNA. J Mol Evol 22:160–174PubMedGoogle Scholar
  18. Higgins DG, Bleasby AJ, Fuchs R (1992) CLUSTAL V: improved software for multiple sequence alignment. CABIOS 8:189–191Google Scholar
  19. Hillis DM (1988) Systematics of the Rana pipiens complex: puzzle and paradigm. Annu Rev Ecol Syst 19:39–63Google Scholar
  20. Iwai T, Nakamura I (1964) Branchial skeleton of the bluefin tuna, with special reference to the gill rays. Bull Misaki Mar Biol Inst Kyoto Univ 6:21–25Google Scholar
  21. Iwai T, Nakamura I, Matsubara K (1965) Taxonomic study of the tunas. Misaki Mar Biol Inst Kyoto Univ [Special Report] 2:1–51Google Scholar
  22. Jones S, Silas EG (1960) Indian tunas—a preliminary review, with a key for their identification. Indian J Fish 7:369–393Google Scholar
  23. Kishino H, Hasegawa M (1989) Evaluation of the maximum likelihood estimate of the evolutionary tree topologies from DNA sequence data, and branching order in Hominoidea. J Mol Evol 29:170–179PubMedGoogle Scholar
  24. Kocher TD, Thomas WK, Mayer A, Edwards SV, Paabo S, Villablanka FX, Wilson AC (1989) Dynamics of mitochondrial DNA evolution in animals: amplification and sequencing with conserved primers. Proc Natl Acad Sci USA 86:6196–6200PubMedGoogle Scholar
  25. Medlin L, Elwood HJ, Stickel S, Sogin ML (1988) The characterization of enzymatically amplified eukaryotic 16S-like rRNA-coding regions. Gene 71:491–499Google Scholar
  26. Nakamura I (1965) Relationships of fishes referable to the subfamily Thunninae on the basis of the axial skeleton. Bull Misaki Mar Biol Inst Kyoto Univ 8:7–38Google Scholar
  27. Powell JR (1983) Interspecific cytoplasmic gene flow in the absence of nuclear gene flow: evidence from Drosophila. Proc Natl Acad Sci USA 80:492–495Google Scholar
  28. Rosen DE (1978) Vicariant patterns and historical explanation in biogeography. Syst Zool 27:159–188Google Scholar
  29. Saitou N, Nei M (1987) The neighbor-joining method: a new method for reconstructing phylogenetic trees. Mol Biol Evol 4:406–425PubMedGoogle Scholar
  30. Sharp GD, Pirages S (1978) The distribution of red and white swimming muscles, their biochemistry, and the biochemical phylogeny of selected scombrid fishes. In: Sharp GD, Dizon AE (eds) The physiological ecology of tunas. Academic Press, New York, pp 41–78Google Scholar
  31. Smith GR (1992) Introgression in fishes: significance for paleontology, cladistics, and evolutionary rates. Syst Biol 41:41–57Google Scholar
  32. Spolsky C, Uzzell T (1984) Natural interspecific transfer of mitochondrial DNA in amphibians. Proc Natl Acad Sci USA 81:5802–5805Google Scholar
  33. Tautz D, Hancock JM, Webb DA, Tautz C, Dover GA (1988) Complete sequences of the rRNA genes of Drosophila melanogaster. Mol Biol Evol 5:366–376Google Scholar

Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • Seinen Chow
    • 1
  • Hirohisa Kishino
    • 2
  1. 1.National Research Institute of Far Seas FisheriesShimizu, ShizuokaJapan
  2. 2.Department of Social and International RelationsUniversity of TokyoKomaba, TokyoJapan

Personalised recommendations