Applied Microbiology and Biotechnology

, Volume 43, Issue 2, pp 211–216

Pyrrolnitrin and phenazine production by Pseudomonas cepacia, strain 5.5B, a biocontrol agent of Rhizoctonia solani

  • D. Kelly Cartwright
  • W. S. Chilton
  • D. M. Benson
Biotechnology Original Paper

Abstract

Rhizoctonia stem rot of poinsettia caused by Rhizoctonia solani is controlled by strain 5.5B of Pseudomonas cepacia when poinsettia cuttings are rooted in polyfoam rooting cubes. Experiments were conducted to isolate and characterize secondary metabolites from strain 5.5B that were inhibitory towards R. solani. Inhibitory compounds were detected in fractions processed from liquid cultures of strain 5.5B. The most inhibitory compound isolated was pyrrolnitrin. A purple pigment consistently produced in culture by strain 5.5B was isolated and identified as 4,9-dihydroxyphenazine-1,6-dicarboxylic acid dimethyl ester, a phenazine. In vitro inhibition of R. solani occurred with the phenazine.

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References

  1. Arima K, Imaraka H, Kousaka M, Fukuda A, Tamura G (1964) Pyrrolnitrin, a new antibiotic substance, produced by Pseudomonas. Agric Biol Chem 28:575–576Google Scholar
  2. Bailey DA (1990) Cultural practices and IPM for Poinsettias. North Carolina Flower Growers' Bulletin 35:2–6Google Scholar
  3. Bakker AW, Schippers B (1987) Microbial cyanide production in the rhizosphere in relation to potato yield reduction and Pseudomonas spp.-mediated plant growth-stimulation. Soil Biol Biochem 19:451–457Google Scholar
  4. Bull CT, Weller DM, Thomashow LS (1991) Relationship between root colonization and suppression of Gaeumannomyces graminis var. tritici by Pseudomonas fluorescens strain 2–79. Phytopathology 81:954–959Google Scholar
  5. Campbell R (1989) Direct inoculation of fungal and bacterial antagonists. In: Biological control of microbial plant pathogens. University Press, Cambridge, pp 144–149Google Scholar
  6. Cartwright D Kelly, Benson DM (1992) Biocontrol of Rhizoctonia stem rot of poinsettia (Euphorbia pulcherrima) in polyfoam rooting cubes. (abstract) Phytopathology 82:1120Google Scholar
  7. Cartwright D Kelly, Benson DM (1994) Effect of population dynamics of Pseudomonas cepacia and Paecilomyces lilacinus in polyfoam rooting cubes in relation to colonization by Rhizoctonia solani. Appl Environ Microbiol 60:2852–2857Google Scholar
  8. Cook RJ, Baker KF (1983) Perspectives. In: Cook RJ, Baker KF (eds) The nature and practice of biological control of plant pathogens. The American Phytopathological Society, St. Paul, Minn pp 426–444Google Scholar
  9. Dhingra OD, Sinclair JB (1985) Basic plant pathology methods. CRC, Boca Raton, Fla, p 355Google Scholar
  10. Ecke P, Jr., Matkin OA, Hartley DE (1990) Poinsettia diseases. In: The poinsettia manual. Paul Ecke Poinsettias, Encinitas, Calif, pp 168–170Google Scholar
  11. Flaishman M, Eyal Z, Voisard C, Haas D (1990) Suppression of Septoria tritici by phenazine- or siderophore-deficient mutants of Pseudomonas. Curr Microbiol 20:121–124Google Scholar
  12. Gurusiddaiah S, Weller DM, Sarkar A, Cook RJ (1986) Characterization of an antibiotic produced by a strain of Pseudomonas fluorescens inhibitory to Gaeumannomyces graminis var. tritici and Pythium spp. Antimicrob Agents Chemother 29:488–495Google Scholar
  13. Harrison LA, Letendre L, Kovacevich P, Pierson E, Weller DM (1993) Purification of an antibiotic effective against Gaeumannomyces graminis var. tritici produced by a biocontrol agent, Pseudomonas aureofaciens. Soil Biol Biochem 25:215–221Google Scholar
  14. Homma Y, Suzui T (1989) Role of antibiotic production in suppression of radish damping-off by seed bacterization with Pseudomonas cepacia. Ann Phytopathol Soc Jpn 55:643–652Google Scholar
  15. Howell CR, Stipanovic RD (1979). Control of Rhizoctonia solani on cotton seedlings with Pseudomonas fluorescens and with an antibiotic produced by the bacterium. Phytopathology 69:480–482Google Scholar
  16. Janisiewicz WJ, Roitman J (1988) Biological control of blue mold and gray mold on apple and pear with Pseudomonas cepacia. Phytopathology 78:1697–1700Google Scholar
  17. Jayaswal RK, Fernandez MA, Schroeder RG III (1990) Isolation and characterization of a Pseudomonas strain that restricts growth of various phytopathogenic fungi. Appl Environ Microbiol 56:1053–1058Google Scholar
  18. Jones RK (1990) Poinsettia diseases and their management. N C Flower Growers Bull 35:6–11Google Scholar
  19. Korth H, Romer A, Budzikiewicz H, Pulverer G (1978) 4,9-Dihydroxyphenazine-1,6-dicarboxylic acid dimethyl ester and the ‘missing link’ in phenazine biosynthesis. J Gen Microbiol 104:299–303Google Scholar
  20. Lockwood JL (1984) Soil fungistasis: mechanisms and relation to biological control of soilborne plant pathogens. In: Bay-Peterson J (ed) Soilborne crop diseases in Asia. Food and Fertilizer Technology Center for the Asian and Pacific Book Series, no. 26, Taipei, Taiwan, pp 159–174Google Scholar
  21. Loper JE (1988) Role of fluorescent siderophore production in biological control of Pythium ultimum by a Pseudomonas fluorescens strain. Phytopathology 78:166–172Google Scholar
  22. Morris MB, Roberts JB (1959) A group of pseudomonads able to synthesize poly-β-hydroxy-butyric acid. Nature 183:1538–1539Google Scholar
  23. Powell, CC Jr (1988) The safety and efficacy of fungicides for use in Rhizoctonia crown rot control of directly potted unrooted poinsettia cuttings. Plant Dis 72:693–695Google Scholar
  24. Roitman JN, Mahoney NE, Janisiewicz WJ (1990) Production and composition of phenylpyrrole metabolites produced by Pseudomonas cepacia. Appl. Microbiol. Biotechnol. 34:381–386Google Scholar
  25. Slininger PJ, Jackson MA (1992) Nutritional factors regulating growth and accumulation of phenazine 1-carboxylic acid by Pseudomonas fluorescens 2–79. Appl Microbiol Biotechnol 37:388–392Google Scholar
  26. Smilanick JL, Denis-Arrue R (1992) Control of green mold of lemons with Pseudomonas species. Plant Dis 76:481–485Google Scholar
  27. Staub T, Sozzi D (1984) Fungicide resistance: a continuing challenge. Plant Dis 68:1026–1031Google Scholar
  28. Strider DL, Jones RK (1985) Poinsettias. In: Strider DL (ed.) diseases of floral crops, vol 2. Praeger, New York, pp 351–403Google Scholar
  29. Thomashow LS, Weller DM (1988) Role of a phenazine antibiotic from Pseudomonas fluorescens in biological control of Gaeumannomyces graminis var. tritici. J Bact 170:3499–3508Google Scholar
  30. Thomashow LS, Weller DM, Bonsall RF, Pierson LS III (1990) Production of the antibiotic phenazine-1-carboxylic acid by fluorescent Pseudomonas species in the rhizosphere of wheat. Appl Environ Microbiol 56:908–912Google Scholar
  31. Turner JM, Messenger AJ (1986) Occurrence, biochemistry and physiology of phenazine pigment production. In: Rose AH, Tempest DW (eds) Microbial physiology, vol 27. Academic Press, London, pp 211–275Google Scholar
  32. Tweedy BG (1983) The future of chemicals for controlling plant diseases. In: Kommedahl T, Williams PH (eds) Challenging problems in plant health. The American Phytopathological Society, St. Paul, Minn, pp 405–415Google Scholar
  33. Weller DM (1988) Biological control of soilborne pathogens in the rhizosphere with bacteria. Annu Rev Phytopathol 26:379–407Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • D. Kelly Cartwright
    • 1
  • W. S. Chilton
    • 2
  • D. M. Benson
    • 1
  1. 1.Department of Plant PathologyNorth Carolina State UniversityRaleighUSA
  2. 2.Department of of BotanyNorth Carolina State UniversityRaleighUSA

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