, Volume 42, Issue 6, pp 501–530 | Cite as

Mouse T-cell receptor variable gene segment families

  • Bernhard Arden
  • Stephen P. Clark
  • Dieter Kabelitz
  • Tak W. Mak


All mouse T-cell receptor α/δ, β, and γ variable (Tcra/d, b-, and g-V) gene segments were aligned to compare the sequences with one another, to group them into subfamilies, and to derive a name which complies with the standard nomenclature. It was necessary to change the names of some V gene segments because they conflicted with those of other segments. The traditional classification into subfamilies was re-evaluated using a much larger pool of sequences. In the mouse, most V gene segments can be grouped into subfamilies of closely related genes with significantly less similarity between different subfamilies.


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  1. Acha-Orbea, H., Mitchell, D. J., Timmermann, L., Wraith, D. C., Tausch, G. S., Waldor, M. K., Zamvil, S. S., McDevitt, H. O., and Steinman, L. Limited heterogeneity of T cell receptors from lymphocytes mediating autoimmune encephalomyelitis allows specific immune intervention. Cell 54: 263–273, 1988Google Scholar
  2. Arden, B., Klotz, J. L., Siu, G., and Hood, L. E. Diversity and structure of genes of the α family of mouse T-cell antigen receptor. Nature 316: 783–787, 1985Google Scholar
  3. Arden, B., Clark, S. P., Kabelitz, D., and Mak, T. W. Human T-cell receptor variable gene segment families. Immunogenetics 42: 455–500, 1995PubMedGoogle Scholar
  4. Asarnow, D. M., Kuziel, W. A., Bonyhadi, M., Tigelaar, R. E., Tucker, P. W., and Allison, J. P. Limited diversity of γδ antigen receptor genes of Thy-1+ dendritic epidermal cells. Cell 55: 837–847, 1988Google Scholar
  5. Barth, R. K., Kim, B. S., Lan, N. C., Hunkapiller, T., Sobiek, N., Winoto, A., Garshenfeld, H., Okada, C.,Hansburg, D., Weissman, I. L., and Hood, L. The murine T-cell receptor uses a limited repertoire of expressed V β gene segments. Nature 316: 517–523, 1985Google Scholar
  6. Becker, D. M., Patten, P., Chien, Y. H., Yokota, T., Eshhar, Z., Giedlin, M., Gascoigne, N. R. J., Goodnow, C., Wolf, R., Arai, K. I., and Davis, M. M. Variability and repertoire size of T-cell receptor gene segments. Nature 317: 430–434, 1985Google Scholar
  7. Behlke, M. A., Spinella, D. G., Chou, H. S., Sha, W., Hartl, D. L., and Loh, D. Y. T-cell receptor β-chain expression: dependence on relatively few variable region genes. Science 229: 566–570, 1985Google Scholar
  8. Behlke, M. A. and Loh, D. Y. Alternative splicing of murine T-cell receptor β-chain transcripts. Nature 322: 379–382, 1986Google Scholar
  9. Behlke, M. A., Chou, H. S., Huppi, K., and Loh, D. Y. Murine T-cell receptor mutants with deletions of β-chain variable region genes. Proc Natl Acad Sci USA 83: 761–771, 1986Google Scholar
  10. Bluestone, J. A., Cron, R. Q., Cotterman, M., Houlden, B. A., and Matis, L. A. Structure and specificity of T cell receptor γδ on major histocompatibility complex antigen-specific CD3+, CD4−, CD8− T lymphocytes. J Exp Med 168: 1899–1916, 1988Google Scholar
  11. Cazenave, P. A., Marche, P. N., Jouvin-Marche, E., Voegtlé, D., Bonhomme, F., Bandeira, A., and Coutinho, A. Vβ17 gene polymorphism in wild-derived mouse strains: two amino acid substitutions in the Vβ17 region greatly alter T cell receptor specificity. Cell 63: 717–728, 1990Google Scholar
  12. Chien, Y., Becker, D. M., Lindsten, T., Okamura, M., Cohen, D. I. and Davis, M. M. A third type of murine T-cell receptor gene. Nature 312: 31–35, 1984aGoogle Scholar
  13. Chien, Y., Gascoigne, N. R. J., Kavaler, J., Lee, N. E., and Davis, M. M. Somatic recombination in a murine T-cell receptor gene. Nature 309: 322–326, 1984 bGoogle Scholar
  14. Chien, Y., Iwashima, M., Wettstein, D. A., Kaplan, K. B., Elliott, J. F., Born, W., and Davis, M. M. T-cell receptor δ gene rearrangements in early thymocytes. Nature 330: 722–727, 1987 aGoogle Scholar
  15. Chien, Y., Iwashima, M., Kaplan, K. B., Elliott, J. F., and Davis, M. M. A new T-cell receptor gene located within the alpha locus and expressed early in T-cell differentiation. Nature 327: 677–682, 1987 bGoogle Scholar
  16. Chothia, C., Boswell, D. R., and Lesk, A. M. The outline structure of the T-cell αβ receptor. EMBO J 7: 3745–3755, 1988Google Scholar
  17. Chou, H. S., Behlke, M. A., Godambe, S. A., Russell, J. H., Brooks, C. G., and Loh, D. Y. T cell receptor genes in an alloreactive CTL clone: implications for rearrangement and germline diversity of variable gene segments. EMBO J 5: 2149–2155, 1986Google Scholar
  18. Chou, H. S., Anderson, S. J., Louie, M. C., Godambe, S. A., Pozzi, M. R., Behlke, M. A. Huppi, K., and Loh, D. Y. Tandem linkage and unusual RNA splicing of the T-cell receptor β-chain variable-region genes. Proc Natl Acad Sci USA 84: 1992–1996, 1987Google Scholar
  19. Clark, S. P. MALIGNED: a multiple sequence alignment editor. Comput Appl Biosci 8: 535–538, 1992Google Scholar
  20. Clark, S. P., Arden, B., Kabelitz, D., and Mak, T. W. Comparison of human and mouse T-cell receptor variable gene segment subfamilies. Immunogenetics 42: 531–540, 1995Google Scholar
  21. Couez, D., Malissen, M., Buferne, M., Schmitt-Verhulst, A. M., and Malissen, B. Each of the two productive T cell receptor α-gene rearrangements found in both the A10 and BM 3.3 T cell clones give rise to an α chain which can contribute to the constitution of a surface-expressed α⊙ dimer. Int Immunol 3: 719–729, 1991Google Scholar
  22. Dembic, Z., Haas, W., Weiss, S., McCubrey, J., Kiefer, H., von Boehmer, H., and Steinmetz, M. Transfer of specificity by murine α and β T-cell receptor genes. Nature 320: 232–238, 1986Google Scholar
  23. Devereux, J., Haeberli, P., and Smithies, O. A comprehensive set of sequence analysis programs for the VAX. Nucleic Acids Res 12: 387–395, 1984PubMedGoogle Scholar
  24. Elliott, J. F., Rock, E. P., Patten, P. A., Davis, M. M., and Chien, Y. The adult T-cell receptor δ-chain is diverse and distinct from that of fetal thymocytes. Nature 331: 627–631, 1988Google Scholar
  25. Epplen, J. T., Bartels, F., Becker, A., Nerz, G., Prester, M., Rinaldy, A., and Simon, M. M. Change in antigen specificity of cytotoxic T-lymphocytes is associated with the rearrangement and expression of a T-cell receptor β-chain gene. Proc Natl Acad Sci USA 83: 4441–4445, 1986Google Scholar
  26. Fink, P. J., Matis, L. A., McElligott, D. L., Bookman, M., and Hedrick, S. M. Correlations between T-cell specificity and the structure of the antigen receptor. Nature 321: 219–226, 1986Google Scholar
  27. Fondell, J. D., Marolleau, J. P., Primi, D., and Marcu, K. B. On the mechanism of nonallelically excluded Vα-Jα T cell receptor secondary rearrangements in a murine T cell lymphoma. J Immunol 144: 1094–1103, 1990Google Scholar
  28. Fondell, J. D. and Marcu, K. B. Transcription of germ line Vα segments correlates with ongoing T-cell receptor α-chain rearrangement. Mol Cell Biol 12: 1480–1489, 1992Google Scholar
  29. Furutani, M., Yanagi, Y., Fujisawa, I., Nakayama, T., Kishimoto, H., Kuida, K., Asano, Y., and Tada, T. Post-transcriptional allelic exclusion of two functionally rearranged T cell receptor α genes. Int Immunol 1: 281–288, 1989Google Scholar
  30. Garman, R. D., Doherty, P. J., and Raulet, D. H. Diversity, rearrangement, and expression of murine T cell gamma genes. Cell 45: 733–742, 1986Google Scholar
  31. Gascoigne, N. R. J., Waters, S., Elliot, J. F., Victor-Kobrin, C., Goodnow, C., Davis, M. M., and Bona, C. A., Expression of T cell receptor genes in an antigen-specific hybridoma and radiation-induced variants. J Exp Med 164: 113–130, 1986Google Scholar
  32. Goodman, T., LeCorre, R., and Lefrancois, L. A T-cell receptor γδ-specific monoclonal antibody detects a Vγ5 region polymorphism. Immunogenetics 35: 56–68, 1992Google Scholar
  33. Goverman, J., Minard, K., Shastri, N., Hunkapiller, T., Hansburg, D., Sercarz, E., and Hood, L. Rearranged β T cell receptor genes in a helper T cell clone specific for lysozyme: no correlation between Vβ and MHC restriction. Cell 40: 859–867, 1985Google Scholar
  34. Hagiya, M., Davis, D. D., Shultz, L. D., and Sakano, H. Non-germ-line elements (NGE) are present in the T cell receptor β-chain genes isolated from the mutant mouse, motheaten (me/me). J Immunol 136: 2697–2700, 1986Google Scholar
  35. Happ, M. P., Kubo, R. T., Palmer, E., Born, W. K., and O'Brien, R. L. Limited receptor repertoire in a mycobacteria-reactive subset of γδ T lymphocytes. Nature 342: 696–698, 1989Google Scholar
  36. Happ, M. P. and Palmer, E. Thymocyte development: an analysis of T cell receptor gene expression in 519 newborn thymocyte hybridomas. Eur J Immunol 19: 1317–1325, 1989Google Scholar
  37. Hayday, A. C., Diamond, D. J., Tanigawa, G., Heilig, J. S., Folsom, V., Saito, H., and Tonegawa, S. Unusual organization and diversity of T-cell receptor α-chain genes. Nature 316: 828–832, 1985 aGoogle Scholar
  38. Hayday, A. C., Saito, H., Gillies, S. D., Kranz, D. M., Tanigawa, G., Eisen, H. N, and Tonegawa, S. Structure, organization, and somatic rearrangement of T cell gamma genes. Cell 40: 259–269, 1985 bGoogle Scholar
  39. Hedrick, S. M., Nielsen, E. A., Kavaler, J., Cohen, D. I., and Davis, M. M., Sequence relationships between putative T-cell receptor polypeptides and immunoglobulins. Nature 308: 153–158, 1984Google Scholar
  40. Hedrick, S. M., Engel, I., McElligott, D. L., Fink, P. J., Hsu, M.-L., Hansburg, D., and Matis, L. A. Selection of amino acid sequences in the beta chain of the T cell antigen receptor. Science 239: 1541–1544, 1988Google Scholar
  41. Heilig, J. S. and Tonegawa, S. Diversity of murine gamma genes and expression in fetal and adult T lymphocytes. Nature 322: 836–840, 1986Google Scholar
  42. Heuer, J., Degwert, J., Pauels, H. G., and Koelsch, E. T cell receptor α and β gene expression in a murine antigen-specific T suppressor lymphocyte clone with cytolytic potential. J Immunol 146: 775–782, 1991Google Scholar
  43. Hirama, T., Takeshita, S., Matsubayashi, Y., Iwashiro, M., Masuda, T., Kuribayashi, K., Koshida, Y., and Yamagishi, H. Conserved V(D)J junctional sequence of crossreactive cytotoxic T-cell receptor idiotype and the effect of a single amino acid substitution. Eur J Immunol 21: 483–488, 1991Google Scholar
  44. Hochgeschwender, U., Simon, H. G., Weltzien, H. U., Bartels, F., Becker, A., and Epplen, J. T., Dominance of one T-cell receptor in the H-2Kb/TNP responce. Nature 326: 307–309, 1987Google Scholar
  45. Hue, I., Trucy, J., McCoy, C., Couez, D., Malissen, B., and Malissen, M. A novel type of aberrant T cell receptor α-chain gene rearrangement: implications for allelic exclusion and the V-J recombination process. J Immunol 144: 4410–4419, 1990Google Scholar
  46. Iwamoto, A., Rupp, F., Ohashi, P. S., Walker, C. L., Pircher, H., Joho, R., Hengartner, H., and Mak, T. W. T cell-specific γ genes in C57BL/10 mice. Sequence and expression of new constant and variable region genes. J Exp Med 163: 1203–1212, 1986Google Scholar
  47. Iwamoto, A., Ohashi, P. S., Pircher, H., Walker, C. L., Michalopoulos, E. E., Rupp, F., Hengartner, H., and Mak, T. W. T cell receptor variable gene usage in a specific cytotoxic T cell response. Primary structure of the antigen-MHC receptor of four hapten-specific cytotoxic T cell clones. J Exp Med 165: 591–600, 1987Google Scholar
  48. Iwasato, T. and Yamagishi, H. Novel excision products of T cell receptor γ gene rearrangements and developmental stage specificity implied by the frequency of nucleotide insertions at signal joints. Eur J Immunol 22: 101–106, 1992Google Scholar
  49. Iwashima, M., Green, A., Davis, M. M., and Chien, Y. Variable region (Vδ) gene segment most frequently utilized in adult thymocytes is 3′ of the constant (Cδ) region. Proc Natl Acad Sci USA 85: 8161–8165, 1988Google Scholar
  50. Jameson, S. C., Nakajima, P. B., Brooks, J. L., Heath, W., Kanagawa, O., and Gascoigne, N. R. J. The T cell receptor Vα11 gene family: analysis of allelic sequence polymorphism and demonstration of Jα region-dependent recognition by allele-specific antibodies. J Immunol 147: 3185–3193, 1991Google Scholar
  51. Johnson, N. A., Carland, F., Allen, P. M., and Glimcher, L. H. T cell receptor gene segment usage in a panel of hen-egg white lysozyme specific, I-Ak-restricted T helper hybridomas. J Immunol 142: 3298–3304, 1989Google Scholar
  52. Kalataradi, H., Eyster, C. L., Fry, A., Vollmer, M. K., Fu, Y.-X., Born, W. K., and O'Brien, R. L. Allelic differences in TCR γ-chains alter γδ T cell antigen reactivity. J Immunol 153: 1455–1465, 1994Google Scholar
  53. Kappler, J. W., Wade, T., White, J., Kushnir, E., Blackman, M., Bill, J., Roehm, N., and Marrack, P. A T cell receptor Vβ segment that imparts reactivity to a class II major histocompatibility complex product. Cell 49: 263–271, 1987Google Scholar
  54. Kishihara, K., Yoshikai, Y., Matsuzaki, G., Mak, T. W., and Nomoto, K. Functional α and β T cell chain receptor messages can be detected in old but not in young athymic mice. Eur J Immunol 17: 477–482, 1987Google Scholar
  55. Klotz, J. L., Barth, R. K., Kiser, G. L., Hood, L. E., and Kronenberg, M. Restriction fragment length polymorphisms of the mouse T-cell receptor gene families [published erratum appears in Immunogenetics 30: 235, 1989]. Immunogenetics 29: 191–201, 1989Google Scholar
  56. Koop, B. F., Wilson, R. K., Wang, K., Vernooij, B., Zaller, D., Kuo, C. L., Seto, D., Toda, M., and Hood, L. Organization, structure, and function of 95 kb of DNA spanning the murine T-cell receptor Cα/Cδ region. Genomics 13: 1209–1230, 1992Google Scholar
  57. Korman, A. J., Marusic-Galesic, S., Spencer, D., Kruisbeek, A. M., and Raulet, D. H. Predominant variable region gene usage by γ/δ T cell receptor-bearing cells in the adult thymus. J Exp Med 168: 1021–1040, 1988Google Scholar
  58. Korman, A. J., Maruyama, J., and Raulet, D. H., Rearrangement by inversion of a T-cell receptor δ variable region gene located 3' of the δ constant region gene. Proc Natl Acad Sci USA 86: 267–271, 1989Google Scholar
  59. Koseki, H., Imai, K., Ichikawa, T., Hayata, I., and Taniguchi, M. Predominant use of a particular α-chain in suppressor T cell hybridomas specific for keyhole limpet hemocyanin. Int Immunol 1: 557–564, 1989Google Scholar
  60. Kraig, E., Kannapell, C. C., Zborowski, K. A., Pierce, C. W., and Kapp, J. A. Sequence of the TCR β chain gene used by a T cell clone specific for the synthetic polymer, GAT. Nucleic Acids Res 18: 5881, 1990Google Scholar
  61. Kranz, D. M., Saito, H., Heller, M., Takagaki, Y., Haas, W., Eisen, H. N., and Tonegawa, S. Limited diversity of the rearranged T-cell γ gene. Nature 313: 752–755, 1985Google Scholar
  62. Lai, M. Z., Huang, S. Y., Briner, T. J., Guillet, J. G., Smith, J. A., and Gefter, M. L. T cell receptor gene usage in the response to λ repressor cI protein: an apparent bias in the usage of a Vα gene element. J Exp Med 168: 1081–1097, 1988Google Scholar
  63. Lai, E., Wilson, R. K., and Hood, L. E. Physical maps of the mouse and human immunoglobulin-like loci. Adv Immunol 46: 1–59, 1988Google Scholar
  64. Lee, N. E., and Davis, M. M. T cell receptor β-chain genes in BW5147 and other AKR tumors: deletion order of murine Vβ gene segments and possible 5' regulatory regions. J Immunol 140: 1665–1675, 1988Google Scholar
  65. Letourneur, F. and Malissen, B. Derivation of a T cell hybridoma variant deprived of functional T cell receptor α and β chain transcripts reveals a nonfunctional α-mRNA of BW5147 origin. Eur J Immunol 19: 2269–2274, 1989Google Scholar
  66. Louie, M. C., Nelson, C. A., and Loh, D. Y., Identification and characterization of new murine T cell receptor β-chain variable region (Vβ) genes. J Exp Med 170: 1987–1998, 1989Google Scholar
  67. Lyon, M. F. Rules and guidelines for gene nomenclature. In M. F. Lyon and A. G. Searle (eds.): Genetic Variants and Strains of the Laboratory Mouse, 2nd edn, pp. 1–11, Oxford University Press, Oxford, 1989Google Scholar
  68. Malissen, M., McCoy, C., Blanc, D., Trucy, J., Devaux, C., Schmitt-Verhulst, A. M., Fitch, F., Hood, L., and Malissen, B. Direct evidence for chromosomal inversion during T-cell receptor β-gene rearrangements. Nature 319: 28–33, 1986Google Scholar
  69. Malissen, M., Trucy, J., Letourneur, F., Rebaï, N., Dunn, D. E., Fitch, F. W., Hood, L., and Malissen, B. A T cell clone expresses two T cell receptor α genes but uses one αβ heterodimer for allorecognition and self MHC-restricted antigen recognition. Cell 55: 49–59, 1988Google Scholar
  70. Marolleau, J. P., Fondell, J. D., Malissen, M., Trucy, J., Barbier, E., Marcu, K. B., Cazenave, P. A., and Primi, D. The joining of germ-line Vα to Jα genes replaces the preexisting Vα-Jα complexes in a T cell receptor α,β positive T cell line. Cell 55: 291–300, 1988Google Scholar
  71. McConnell, T. J., Yokoyama, W. M., Kikuchi, G. E., Einhorn, G. P., Stingl, G., Shevach, E. M., and Coligan, J. E. δ-chains of dendritic epidermal T cell receptors are diverse but pair with γ-chains in a restricted manner. J Immunol 142: 2924–2931, 1989Google Scholar
  72. McElligott, D. L., Sorger, S. B., Matis, L. A., and Hedrick, S. M., Two distinct mechanisms account for the immune response (Ir) gene control of the T cell response to pigeon cytochrome c. J Immunol 140: 4123–4131, 1988Google Scholar
  73. Morahan, G., Allison, J., Peterson, M. G., and Malcolm, L. Sequence of the V β13 gene used by an influenza-specific T cell. Immunogenetics 30: 311–313, 1989Google Scholar
  74. Mori, L., Ricciardi-Castagnoli, P., and Steinmetz, M. A mouse T lymphoma that lacks T-cell receptor surface expression exhibits allelic exclusion of its β chain genes. Immunogenetics 28: 136–138, 1988Google Scholar
  75. Morinaga, T., Fotedar, A., Singh, B., Wegmann, T. G., and Tamaoki, T. Isolation of cDNA clones encoding a T-cell receptor β-chain from a beef insulin-specific hybridoma. Proc Natl Acad Sci USA 82: 8163–8167, 1985Google Scholar
  76. Okazaki, K. and Sakano, H. Thymocyte circular DNA excised from T cell receptor α-δ gene complex. EMBO J 7: 1669–1674, 1988Google Scholar
  77. Palmer, M. S., Bentley, A., Gould, K., and Townsend, A. R. M. The T-cell receptor from an influenza-A specific murine CTL clone. Nucleic Acids Res 17: 2353, 1989Google Scholar
  78. Patten, P., Yokota, T., Rothbard, J., Chien, Y. H., Arai, K. I., and Davis, M. M. Structure, expression and divergence of T-cell receptor β-chain variable regions. Nature 312: 40–46, 1984Google Scholar
  79. Pelkonen, J., Traunecker, A., and Karjalainen, K. A new mouse TCR Vγ gene that shows remarkable evolutionary conservation. EMBO0 J 6: 1941–1944, 1987Google Scholar
  80. Pircher, H., Michalopoulos, E. E., Iwamoto, A., Ohashi, P. S., Baenziger, J., Hengartner, H., Zinkernagel, R. M., and Mak, T. W., Molecular analysis of the antigen receptor of virus-specific cytotoxic T cells and identification of a new Vα family. Eur J Immunol 17: 1843–1846, 1987Google Scholar
  81. Pullen, A. M., Potts, W., Wakeland, E. K., Kappler, J., and Marrack, P., Surprisingly uneven distribution of the T cell receptor Vβ repertoire in wild mice. J Exp Med 171: 49–62, 1990Google Scholar
  82. Raulet, D. H. The structure, function, and molecular genetics of the γ/δ T cell receptor. Annu Rev Immunol 7: 175–207, 1989CrossRefPubMedGoogle Scholar
  83. Rinaldy, A., Wallace, R. B., Simon, M. M., Becker, A., and Epplen, J. T. A highly homologous T-cell receptor β-chain variable region is expressed in mouse and human T cells. Immunogenetics 21: 403–406, 1985Google Scholar
  84. Roger, T., and Seman, M. Polymorphism of the Tcrg-V1-V2 region in mice: identification of a new Vg1 allele in DBA/2. Immunogenetics 36: 67–69, 1992Google Scholar
  85. Roger, T., Miconnet, I., Bruley-Rosset, M., and Seman, M. Coding sequence polymorphism of Tcrg-V1, -V2, and -V4 genes in mice bearing Tcr-gA and -gC haplotypes. Immunogenetics 39: 68–70, 1994Google Scholar
  86. Roger, T., Morisset, J., and Seman, M. Conservation of Tcrg-V5 and limited allelic sequence polymorphism of the other Tcrg-V genes used by mouse tissue-specific γδ-T lymphocytes. Immunogenetics, in pressGoogle Scholar
  87. Rupp, F., Acha-Orbea, H., Hengartner, H., Zinkernagel, R., and Joho, R. Identical V β T-cell receptor genes used in alloreactive cytotoxic and antigen plus I-A specific helper T cells. Nature 315: 425–427, 1985Google Scholar
  88. Rupp, F., Frech, G., Hengartner, H., Zinkernagel, R. M., and Joho, R. No functional γ-chain transcripts detected in an alloreactive cytotoxic T-cell clone. Nature 321: 876–878, 1986Google Scholar
  89. Rupp, F., Brecher, J., Giedlin, M. A., Mosmann, T., Zinkernagel, R. M., Hengartner, H., and Joho, R. H. T-cell antigen receptors with identical variable regions but different diversity and joining region gene segments have distinct specificities but cross-reactive idiotypes. Proc Natl Acad Sci USA 84: 219–222, 1987Google Scholar
  90. Saito, H., Kranz, D. M., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S. A third rearranged and expressed gene in a clone of cytotoxic T lymphocytes. Nature 312: 36–40, 1984aGoogle Scholar
  91. Saito, H., Kranz, D. M., Takagaki, Y., Hayday, A. C., Eisen, H. N., and Tonegawa, S. Complete primary structure of a heterodimeric T-cell receptor deduced from cDNA sequences. Nature 309: 757–762, 1984bGoogle Scholar
  92. Seto, D., Koop, B. F., Deshpande, P., Howard, S., Seto, J., Wilk, E., Wang, K., and Hood, L. Organization, sequence, and function of 34.5 kb of genomic DNA encompassing several murine T-cell receptor α/δ variable gene segments. Genomics 20: 258–266, 1994Google Scholar
  93. Sherman, D. H., Hochman, P. S., Dick, R., Tizard, R., Ramachandran, K. L., Flavell, R. A., and Huber, B. T. Molecular analysis of antigen recognition by insulin-specific T-cell hybridomas from B6 wild-type and bm 12 mutant mice. Mol Cell Biol 7: 1865–1872, 1987Google Scholar
  94. Singer, P. A., McEvilly, R. J., Noonan, D. J., Dixon, F. J., and Theofilopoulos, A. N. Clonal diversity and T-cell receptor β-chain variable gene expression in enlarged lymph nodes of MRL-lpr/lpr lupus mice. Proc Natl Acad Sci USA 83: 7018–7022, 1986Google Scholar
  95. Six, A., Jouvin-Marche, E., Loh, D. Y., Cazenave, P. A., and Marche, P. N. Identification of a T cell receptor β chain variable region, Vβ20, that is differentially expressed in various strains of mice. J Exp Med 174: 1263–1266, 1991Google Scholar
  96. Smith, L. R., Plaza, A., Singer, P. A., and Theofilopoulos, A. N. Coding sequence polymorphisms among Vβ T cell receptor genes. J Immunol 144: 3234–3237, 1990Google Scholar
  97. Spinella, D. G., Hansen, T. H., Walsh, W. D., Behlke, M. A., Tillinghast, J. P., Chou, H. S., Whiteley, P. J., Kapp, J. A., Pierce, C. W., Shevach, E. M., and Loh, D. Y. Receptor diversity of insulin-specific T cell lines from C57BL (H-2b) mice. J Immunol 138: 3991–3995, 1987Google Scholar
  98. Stoehr, P. J. and Omond, R. A. The EMBL Network File Server. Nucleic Acids Res 17: 6763–6764, 1989Google Scholar
  99. Stoehr, P. J. and Cameron, G. N. The EMBL data library. Nucleic Acids Res 19 (Suppl): 2227–2230, 1991Google Scholar
  100. Sutherland, R. M., Paterson, Y., Scherle, P. A., Gerhard, W., and Caton, A. J. A new mouse T-cell receptor α chain variable region family. Immunogenetics 34: 372–375, 1991Google Scholar
  101. Takagaki, Y., Nakanishi, N., Ishida, I., Kanagawa, O., and Tonegawa, S. T cell receptor-γ and −δ genes preferentially utilized by adult thymocytes for the surface expression. J Immunol 142: 2112–2121, 1989Google Scholar
  102. Takeshita, S., Toda, M., and Yamagishi, H. Excision products of the T cell receptor gene support a progressive rearrangement model of the α/δ locus. EMBO J 8: 3261–3270, 1989Google Scholar
  103. Tan, K. N., Datlof, B. M., Gilmore, J. A., Kronman, A. C., Lee, J. H., Maxam, A. M., and Rao, A. The T cell receptor Vα3 gene segment is associated with reactivity to p-azobenzenearsonate. Cell 54: 247–261, 1988Google Scholar
  104. Taylor, A. H., Haberman, A. M., Gerhard, W., and Caton, A. J. Structure-function relationships among highly diverse T cells that recognize a determinant from influenza virus hemagglutinin. J Exp Med 172: 1643–1651, 1990Google Scholar
  105. Thompson, J. D., Higgins, D. G., and Gibson, T. J. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680, 1994PubMedGoogle Scholar
  106. Toda, M., Fujimoto, S., Iwasato, T., Takeshita, S., Tezuka, K., Ohbayashi, T., and Yamagashi, H. Structure of extrachromosomal circular DNAs excised from T-cell antigen receptor alpha- and delta-chain loci. J Mol Biol 202: 219–231, 1988Google Scholar
  107. Traunecker, A., Oliveri, F., Allen, N., and Karjalainen, K. Normal T cell development is possible without “functional” γ chain genes. EMBO J 5: 1589–1593, 1986Google Scholar
  108. Uematsu, Y., Ryser, S., Dembic, Z., Borgulya, P., Krimpenfort, P., Berns, A., von Boehmer, H., and Steinmetz, M. In transgenic mice the introduced functional T cell receptor β gene prevents expression of endogenous β genes. Cell 52: 831–841, 1988Google Scholar
  109. Uematsu, Y. Preferential association of α and β chains of the T cell antigen receptor. Eur J Immunol 22: 603–606, 1992Google Scholar
  110. Urban, J. L., Kumar, B., Kono, D. H., Gomez, C., Horvath, S. J., Clayton, J., Ando, D. G., Sercarz, E. E., and Hood, L. Restricted use of T cell receptor V genes in murine autoimmune encephalomyelitis raises possibilities for antibody therapy. Cell 54: 577–592, 1988Google Scholar
  111. Von Heijne, G. A new method for predicting signal sequence cleavage sites. Nucleic Acids Res 14: 4683–4690, 1986PubMedGoogle Scholar
  112. Wade, T., Bill, J., Marrack, P. C., Palmer, E., and Kappler, J. W. Molecular basis for the nonexpression of Vβ17 in some strains of mice. J Immunol 141: 2165–2167, 1988Google Scholar
  113. Williams, A. F., Strominger, J. L., Bell, J., Mak, T. W., Kappler, J., Marrack, P., Arden, B., Lefranc, M. P., Hood, L., Tonegawa, S., and Davis, M. Nomenclature for T-cell receptor (TCR) gene segments of the immune system. WHO Bull 71: 113–115, 1993Google Scholar
  114. Winoto, A., Mjolsness, S., and Hood, L. Genomic organization of the genes encoding mouse T-cell receptor α-chain. Nature 316: 832–836, 1985Google Scholar
  115. Winoto, A., Urban, J. L., Lan, N. C., Goverman, J., Hood, L., and Hansburg, D. Predominant use of a V α gene segment in mouse T-cell receptors for cytochrome c. Nature 324: 679–682, 1986Google Scholar
  116. Yague, J., Blackman, M., Born, W., Marrack, P., Kappler, J., and Palmer, E. The structure of Vα and Jα segments in the mouse. Nucleic Acids Res 16: 11355–11364, 1988Google Scholar
  117. Yanagi, Y., Maekawa, R., Cook, T., Kanagawa, O., and Oldstone, M. B. A. Restricted V-segment usage in T-cell receptors from cytotoxic T Lymphocytes specific for a major epitope of lymphocytic choriomeningitis virus. J Virol 64: 5919–5926, 1990Google Scholar
  118. Yoshikai, Y., Reis, M. D., and Mak, T. W. Athymic mice express a high level of functional γ-chain but greatly reduced levels of α- and β-chain T-cell receptor messages. Nature 324: 482–485, 1986Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • Bernhard Arden
    • 1
  • Stephen P. Clark
    • 2
  • Dieter Kabelitz
    • 1
  • Tak W. Mak
    • 3
  1. 1.Paul-Ehrlich-InstituteLangenGermany
  2. 2.Amgen CenterThousand OaksUSA
  3. 3.Amgen InstituteOntario Cancer InstituteToronto OntarioCanada

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