Applied Microbiology and Biotechnology

, Volume 34, Issue 1, pp 141–144 | Cite as

Production and characterization of xylanase from Bacillus thermoalkalophilus grown on agricultural wastes

  • Sridevi Rajaram
  • Ajit Varma
Environmental Microbiology Short Contribution

Summary

Bacillus thermoalkalophilus isolated from termite-infested mound soils of the semi-arid zones of India had the ability to produce good amounts of xylanase(s) from cheap agricultural wastes. Of the two hemicellulosic substrates tested, bagasse was found to be the better inducer for xylanase production. Alkali treatment of bagasse and rice husk had varied effects on enzyme production. The enzyme preparation had activity optima at 60° C and 70° C and a half-life of 60 min at 65° C. The enzyme was stable for 24 h over a pH range of 4.0–6.0, while maximum activity was observed at pH 6.0–7.0. Enzyme production and activity were inhibited by the end-product of xylan hydrolysis, xylose.

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Copyright information

© Springer-Verlag 1990

Authors and Affiliations

  • Sridevi Rajaram
    • 1
  • Ajit Varma
    • 1
  1. 1.Microbiology Unit, School of Life SciencesJawaharlal Nehru UniversityNew DelhiIndia

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