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Applied Microbiology and Biotechnology

, Volume 40, Issue 1, pp 17–22 | Cite as

Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentations using a complex medium

  • Luc De Vuyst
  • Erick J. Vandamme
Article

Abstract

The influence of different phosphorus and nitrogen sources on Lactococcus lactis subsp. lactis NIZO 22186 growth and nisin production was studied in batch fermentations using a complex medium. KH2PO4 was found to be the best phosphorus source for nisin production. Increasing initial phosphate concentrations from 0 to 5% KH2PO4 exerted a double effect, creating favourable pH conditions and particularly stimulating the nisin production levels, which were highest at 5% KH2PO4. Up to now, no such high initial phosphate concentrations have been reported for the production of other antibiotics or bacteriocins. Nisin, a lanthionine-containing peptide antibiotic with bacteriocin properties, clearly behaved as a primary metabolite, since its formation was linked with active growth and was not suppressed by phosphate concentrations up to 5%. A complex medium supplemented with cotton seed meal as nitrogen source also gave very high nisin yields.

Keywords

Nitrogen Source Lactis Batch Fermentation Complex Medium Cotton Seed 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Luc De Vuyst
    • 1
  • Erick J. Vandamme
    • 1
  1. 1.Laboratory of General and Industrial Microbiology, Faculty of Agricultural and Applied Biological SciencesUniversity of GentGentBelgium

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