Applied Microbiology and Biotechnology

, Volume 40, Issue 1, pp 17–22 | Cite as

Influence of the phosphorus and nitrogen source on nisin production in Lactococcus lactis subsp. lactis batch fermentations using a complex medium

  • Luc De Vuyst
  • Erick J. Vandamme


The influence of different phosphorus and nitrogen sources on Lactococcus lactis subsp. lactis NIZO 22186 growth and nisin production was studied in batch fermentations using a complex medium. KH2PO4 was found to be the best phosphorus source for nisin production. Increasing initial phosphate concentrations from 0 to 5% KH2PO4 exerted a double effect, creating favourable pH conditions and particularly stimulating the nisin production levels, which were highest at 5% KH2PO4. Up to now, no such high initial phosphate concentrations have been reported for the production of other antibiotics or bacteriocins. Nisin, a lanthionine-containing peptide antibiotic with bacteriocin properties, clearly behaved as a primary metabolite, since its formation was linked with active growth and was not suppressed by phosphate concentrations up to 5%. A complex medium supplemented with cotton seed meal as nitrogen source also gave very high nisin yields.


Nitrogen Source Lactis Batch Fermentation Complex Medium Cotton Seed 
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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Luc De Vuyst
    • 1
  • Erick J. Vandamme
    • 1
  1. 1.Laboratory of General and Industrial Microbiology, Faculty of Agricultural and Applied Biological SciencesUniversity of GentGentBelgium

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