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Applied Microbiology and Biotechnology

, Volume 40, Issue 2–3, pp 215–218 | Cite as

Large-scale production of anthocyanin by Aralia cordata cell suspension cultures

  • Yoshinori Kobayashi
  • Motomu Akita
  • Kazuo Sakamoto
  • Hongfeng Liu
  • Takeo Shigeoka
  • Takashi Koyano
  • Michio Kawamura
  • Tsutomu Furuya
Biotechnology

Abstract

The suspension culture of high anthocyanin-producing Aralia cordata cell lines, which grow and produce anthocyanin without light irradation, was scaled up from flasks to a 10-1 glass jar fermentor, a 95-1 stainless steel jar fermentor, and finally a 500-l pilot-scale jar fermentor. By the administration of CO2, cell damage was completely prevented and the anthocyanin content was kept as high as 7.0–17.2% (w/w) of the dried cells. In one of the operations of the 500-l jar fermentor, cells were cultivated for 16 days. During this operation, cell mass was increased by more than 26 times (cell yield: 69.2 kg fresh wt.) and the amount of anthocyanin increased by more than 55 times (anthocyanin yield: 545 g, anthocyanin content: 17.2% of the dried cells).

Keywords

Stainless Steel Cell Suspension Suspension Culture Cell Damage Cell Mass 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1993

Authors and Affiliations

  • Yoshinori Kobayashi
    • 1
  • Motomu Akita
    • 1
  • Kazuo Sakamoto
    • 2
  • Hongfeng Liu
    • 1
  • Takeo Shigeoka
    • 1
  • Takashi Koyano
    • 2
  • Michio Kawamura
    • 1
  • Tsutomu Furuya
    • 3
  1. 1.Tsukuba Laboratory, P.C.C. Technology Inc., c/o Kyowa Hakko Kogyo Co., Ltd.Tsukuba Research LaboratoriesTukuba-city, IbaragiJapan
  2. 2.Saitama Laboratory, P.C.C. Technology Inc., c/o Tonen CorporationIruma-gun, SaitamaJapan
  3. 3.School of Pharmaceutical SciencesKitasato UniversityTokyoJapan

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