Applied Microbiology and Biotechnology

, Volume 42, Issue 1, pp 158–166 | Cite as

Cometabolic degradation of chloroallyl alcohols in batch and continuous cultures

  • J. J. van der Waarde
  • R. Kok
  • D. B. Janssen
Environmental Biotechnology Original paper
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Revised:
Accepted:

Abstract

The biodegradation of chloroallyl alcohols by pure and mixed bacterial cultures was investigated. Only 2-chloroallyl alcohol and cis- and trans-3-chloroallyl alcohol served as growth substrate for pure cultures. The other chloroallyl alcohols could be cometabolically degraded during growth on 2-chloroallyl alcohol. Cometabolic degradation of trichloroallyl alcohol, which was the most recalcitrant congener, by a Pseudomonas strain isolated on 2-chloroallyl alcohol resulted in 60% dechlorination. Efficient degradation of a mixture of chloroallyl alcohols in continuous culture could only be achieved in the presence of a satellite population. The mixed culture degraded 99% of the total chloroallyl alcohols added with 71% chloride release. The culture contained strains with a new catabolic potential. The results indicate the importance of mixed cultures and genetic adaptation for efficient chloroallyl alcohol removal.

Keywords

Alcohol Chloride Biodegradation Pseudomonas Bacterial Culture 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • J. J. van der Waarde
    • 1
  • R. Kok
    • 1
  • D. B. Janssen
    • 1
  1. 1.Department of BiochemistryUniversity of GroningenGroningenThe Netherlands

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