Applied Microbiology and Biotechnology

, Volume 36, Issue 4, pp 515–517 | Cite as

Controlled biosynthesis of Val7- and Leu7-surfactins

  • Françoise Peypoux
  • Georges Michel
Applied Microbial and Cell Physiology


Surfactin was found to consist of a mixture of two groups of homologous lipopeptides differing by their peptide sequence; Val7-surfactin was recently characterized as a minor companion of the previously described Leu7-surfactin. The addition of various α-amino acids to the culture media led to variations in the production ratios of the two congeners. The supplementation of l-valine or l-isoleucine to the culture medium resulted in a selective enhancement of the production of the Val7-surfactin whereas this production was very low when l-leucine was the nitrogen source in the culture medium.


Nitrogen Peptide Val7 Nitrogen Source Peptide Sequence 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Arima K, Kakinuma A, Tamura G (1968) Surfactin, a crystalline peptide lipid surfactant produced by Bacillus subtilis: isolation, characterization and its inhibition of fibrin clot formation. Biochem Biophys Res Commun 31:488–494Google Scholar
  2. Bernheimer AW, Avigad LS (1970) Nature and properties of a cytolytic agent produced by Bacillus subtilis. J Gen Microbiol 61:361–369Google Scholar
  3. Cooper DG (1986) Biosurfactants. Microbiol Sci 3:145–149Google Scholar
  4. Cooper DG, MacDonald CR, Duff SJB, Kosaric N (1981) Enhancement production of surfactin from Bacillus subtilis by continuous product removal and metal cation additions. Appl Environ Microbiol 42:408–412Google Scholar
  5. Ghuysen JM, Tipper DJ, Strominger JL (1966) Enzymes that degrade bacterial cell walls. Methods Enzymol 8:695Google Scholar
  6. Kakinuma A, Siguno H, Isono M, Tamura G, Arima K (1969) Determination of fatty acid in surfactin and elucidation of the total structure of surfactin. Agric Biol Chem 33:973–976Google Scholar
  7. Kaneda T (1977) Fatty acids of the genus Bacillus subtilis. Bacteriol Rev 41:391–418Google Scholar
  8. Kluge B, Vater J, Salnikow J, Eckart K (1988) Studies on the biosynthesis of surfactin, a lipopeptide antibiotic from Bacillus subtilis ATCC 21332. FEMS Lett 231:107–110Google Scholar
  9. Landy M, Warren GH, Rosenman SB, Colio LG (1948) Bacillomycin, an antibiotic from Bacillus subtilis active against pathogenic fungi. Proc Soc Exp Biol Med 67:530–541Google Scholar
  10. Nakano MM, Marahiel MA, Zuber P (1988) Identification of a genetic locus required for biosynthesis of the lipopeptide antibiotic surfactin in Bacillus subtilis. J Bacteriol 170:5662–5668Google Scholar
  11. Peypoux F, Guinand M, Michel G, Delcambe L, Das BC, Lederer E (1978) Structure of iturin A, a peptidolipid antibiotic from Bacillus subtilis. Biochemistry 17:3992–3996Google Scholar
  12. Peypoux F, Bonmatin JM, Labbe H, Das BC, Ptak M, Michel G (1991) Isolation and characterization of a new variant of surfactin, the Val7-surfactin. Eur J Biochem (in press)Google Scholar
  13. Sandrin C, Peypoux F, Michel G (1990) Coproduction of surfactin and iturin A, lipopeptides with surfactant and antifungal properties, by Bacillus subtilis. Biotechnol Appl Biochem 12:370–375Google Scholar
  14. Swinburne TR, Barr JG, Brown AE (1975) Production of antibiotics by Bacillus subtilis and their effect on fungal colonists of apple leaf scars. Trans Br Mycol Soc 65:211–217Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Françoise Peypoux
    • 1
  • Georges Michel
    • 1
  1. 1.Laboratoire de Biochimie MicrobienneUniversité Claude Bernard Lyon IVilleurbanne CedexFrance

Personalised recommendations