Applied Microbiology and Biotechnology

, Volume 44, Issue 3–4, pp 444–450 | Cite as

Genetic transformation of the pathogenic fungus Wangiella dermatitidis

  • M. Peng
  • C. R. CooperJr.
  • P. J. Szaniszlo
Applied Genetics and Regulation Original Paper


Genetic transformation of Wangiella dermatitidis was studied using three plasmid vectors (pAN7-1, pWU44, and pKK5) and both electroporation and polyethyleneglycol-mediated methods. pAN7-1 contains the E. coli hygromycin B (HmB) phosphotransferase (hph) gene. Expression of the hph gene confers resistance to antibiotic HmB. Selection for resistance, indicative of transformation, resulted in 10–203 HmB-resistant colonies/μg pAN7-1 on medium containing 100 μg HmB/ml. Strains of W. dermatitidis used in this study have innate sensitivity to HmB at a critical inhibitory concentration of 20–40 μg/ml. Vectors pWU44 and pKK5 contain a URA5 gene from Podospora anserina. A ura5 auxotroph of W. dermatitidis was transformed to prototrophy with pWU44 or pKK5 by complementation. Transformation frequencies for these two plasmids were between 17–50 transformants/μg vector DNA. Southern blotting analysis and polymerase chain reaction detection of DNA from putative transformants confirmed transformation.


Polymerase Chain Reaction Southern Blotting Pathogenic Fungus Hygromycin Southern Blotting Analysis 
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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. Peng
    • 1
  • C. R. CooperJr.
    • 2
  • P. J. Szaniszlo
    • 1
  1. 1.Department of MicrobiologyUniversity of Texas at AustinAustinUSA
  2. 2.Department of PathologyUniversity of Texas Medical BranchGalvestonUSA

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