Applied Microbiology and Biotechnology

, Volume 44, Issue 3–4, pp 439–443

Hybridization of genes involved in aflatoxin biosynthesis to DNA of aflatoxigenic and non-aflatoxigenic aspergilli

  • M. A. Klich
  • J. Yu
  • P.-K. Chang
  • E. J. Mullaney
  • D. Bhatnagar
  • T. E. Cleveland
Applied Genetics and Regulation Original Paper

DOI: 10.1007/BF00169941

Cite this article as:
Klich, M.A., Yu, J., Chang, PK. et al. Appl Microbiol Biotechnol (1995) 44: 439. doi:10.1007/BF00169941

Abstract

Southern blots of DNA from a number of aspergilli belonging to Aspergillus section Flavi, including aflatoxin-producing and non-aflatoxigenic isolates of A. flavus and A. parasiticus, were probed with the aflatoxin pathway genes aflR and omt-1. DNA of all A. flavus, A. parasiticus and A. sojae isolates examined hybridized with both genes. None of the A. oryzae isolates examined hybridized to the aflR probe and one of the three did not hybridize to the omt-1 probe. None of the A. tamarii isolates examined hybridized to either gene. Our results suggest that some isolates in this section do not produce aflatoxin because they lack at least one of the genes necessary for biosynthesis, and that non-producing A. flavus, A. parasiticus and A. sojae strains either lack a gene we did not examine or have genes that are not being expressed.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • M. A. Klich
    • 1
  • J. Yu
    • 1
  • P.-K. Chang
    • 2
  • E. J. Mullaney
    • 1
  • D. Bhatnagar
    • 1
  • T. E. Cleveland
    • 1
  1. 1.Southern Regional Research CenterUSDA, ARSNew OrleansUSA
  2. 2.Department of Cell and Molecular BiologyTulane UniversityNew OrleansUSA

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