Applied Microbiology and Biotechnology

, Volume 35, Issue 6, pp 706–710 | Cite as

Production of docosahexaenoic acid by Thraustochytrium aureum

  • Pratima Bajpai
  • Pramod K. Bajpai
  • Owen P. Ward
Biotechnology

Summary

Thraustochytrium aureum ATCC 34 304 contained approximately 50% of total fatty acids as docosahexaenoic acid (DHA). Lipid content of the biomass was dramatically influenced by medium composition and ranged from 0.3 to 16% of the biomass weight. Increasing the culture medium glucose concentration from 5 to 20 g/l caused the lipid content of the biomass to increase from 2.7 to 16.5% and the DHA yield to increase from 26 to 270 mg/l of whole culture broth. The proportion of DHA in lipids was independent of glucose concentration. The fatty acid profile observed in the high-yielding DHA culture was: 16:0, 19.2%; 18:1, 9.8%; 18:2, 2.4%; 20:4, 4.9%; 20:5, 3.6%; 22:6, 48.5% and others, 7.1%. For DHA production, fungal cultures were incubated on an orbital shaker under light at 25° C for 6 days.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Pratima Bajpai
    • 1
  • Pramod K. Bajpai
    • 1
  • Owen P. Ward
    • 1
  1. 1.Department of BiologyUniversity of WaterlooWaterlooCanada
  2. 2.Chemical and Biochemical Engineering DivisionThapar Corporate Research and Development CentrePatialaIndia

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