Applied Microbiology and Biotechnology

, Volume 38, Issue 1, pp 61–66

Use of lipases in the resolution of racemic ibuprofen

  • Annikka Mustranta
Biochemical Engineering


Resolution of (R,S)-ibuprofen enantiomers by esterification in different organic solvents was studied using Candida cylindracea lipase. This enzyme preparation had high enantiospecificity for S(+)-ibuprofen in the esterification reaction of a racemic ibuprofen with primary alcohols. The esterification yields of secondary alcohols were much lower than those of primary alcohols. Esterification with tertiary alcohols was not observed. The synthesis of esters was profoundly affected by the amount of water in the reaction mixture. C. cylindracea lipase was active only in very hydrophobic solvents. The esterification activity of the lipase was reduced significantly by addition of water. The R- and S-enantiomers of ibuprofen were determined without derivatization by HPLC using a chiral column.


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Annikka Mustranta
    • 1
  1. 1.Biotechnical LaboratoryVTTEspooFinland

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