Applied Microbiology and Biotechnology

, Volume 38, Issue 1, pp 39–45 | Cite as

Production of alginate beads by emulsification/internal gelation. I. Methodology

  • D. Poncelet
  • R. Lencki
  • C. Beaulieu
  • J. P. Halle
  • R. J. Neufeld
  • A. Fournier
Biotechnology

Summary

Small diameter alginate beads (microspheres) were formed via internal gelation of alginate solution emulsified within vegetable oil. Gelation was initiated by addition of an oil-soluble acid thereby reducing the pH of the alginate solution and releasing soluble Ca2+ from the citrate complex. Smooth, spherical, micron-sized beads were formed. The mean diameter ranged from 200 to 1000 μm, controlled by the reactor impeller design and rotational speed. The technique has potential for large-scale and continuous applications in immobilization.

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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • D. Poncelet
    • 1
  • R. Lencki
    • 2
  • C. Beaulieu
    • 1
  • J. P. Halle
    • 3
  • R. J. Neufeld
    • 2
  • A. Fournier
    • 1
  1. 1.INRS-SantéUniversité du QuébecQuébecCanada
  2. 2.Department of Chemical EngineeringMcGill UniversityMontréal, QuébecCanada
  3. 3.Maisonneuve Rosemont HospitalMontréal, QuébecCanada
  4. 4.Department of Food ScienceUniversity of GuelphGuelphCanada

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