Sucrose promotes the functional activity of blood vessels after cryopreservation in DMSO-containing fetal calf serum

  • E. Müller-Schweinitzer
  • P. Ellis


The cryoprotective effect of sucrose has been investigated using 3 different vascular smooth muscle preparations, namely canine saphenous veins and arteries and porcine circumflex coronary arteries following storage in liquid nitrogen (at −196°C). Contractile responses to noradrenaline, 5-HT, prostaglandin F and KCl and relaxant responses to substance P and 5-HT were determined on fresh tissues and after cryostorage in fetal calf serum (FCS) containing either 1.8 M dimethyl sulfoxide (DMSO), or 0.1 M sucrose or both agents combined. The data demonstrate that the addition of sucrose to the DMSO-containing cryomedium promotes the preservation of both contractile and relaxant activity of cryostored blood vessels, though sucrose alone did not confer any noticeable protection.

Key words

Cryopreservation Blood vessels DMSO Sucrose 


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Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • E. Müller-Schweinitzer
    • 1
  • P. Ellis
    • 1
  1. 1.Preclinical Research, Sandoz Pharma AGBaselSwitzerland

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