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Applied Microbiology and Biotechnology

, Volume 34, Issue 5, pp 671–676 | Cite as

Degradation of pyrene byRhodococcus sp. UW1

  • U. Walter
  • M. Beyer
  • J. Klein
  • H. -J. Rehm
Environment Biotechnology

Summary

A Rhodococcus species, designated strain UW1, was isolated from contaminated soil using conventional enrichment and isolation techniques. The isolate was able to use pyrene as sole source of carbon and energy; it mineralized 72% of the pyrene within 2 weeks. During growth a metabolite was detected in the culture fluid and further characterized by UV- and mass spectrometry. There is evidence that this metabolite resulted from a recyclization of the direct meta-ring-fission product of pyrene after dihydroxylation in either the 1,2- or 4,5-position. At pH 7.0 and 30°C Rhodococcus sp. UW1 showed a maximum degradation rate of 0.08 mg pyrene/ml per day, while growing with a doubling time of 30 h. The activity of the initial dioxygenase system was characterized by measuring the oxygen-consumption rates of pyrene-induced resting cells, the maxima of which occurred at pH 7.2 and 45°C. Rhodococcus sp. UW1 could also use phenathrene, anthracene, fluoranthene and chrysene as sole sources of carbon and energy, whereas naphthalene, dibenzofuran, fluorene and dibenzothiophene were only co-metabolized.

Keywords

Pyrene Naphthalene Anthracene Fluoranthene Rhodococcus 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • U. Walter
    • 1
  • M. Beyer
    • 1
  • J. Klein
    • 1
  • H. -J. Rehm
    • 2
  1. 1.Deutsche Motan-Technologie-Gesellschaft für Forschung und Prüfung mbHEssen 13Federal Republic of Germany
  2. 2.Institut für MikrobiologieWestfälische Wilhelms UniversitätMünsterFederal Republic of Germany

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