Microbial Ecology

, Volume 24, Issue 3, pp 243–257

Bacterial activity along a trophic gradient

  • Markus Karner
  • Dragica Fuks
  • Gerhard J. Herndl
Article

Abstract

Bacterial biomass, secondary production, and extracellular enzymatic activity [α-glucosidase and leucine-aminopeptidase, measured as cleavage of artificial fluorogenic substrates 4-methyl umbelliferyl (MVF) α-D-glucopyranoside and L-leucine 7-amido-4-methyl coumarin (MCA)] were measured along a trophic gradient in the Northern Adriatic Sea in four ecologically different situations. Bacterial parameters were compared with chlorophyll a and inorganic and organic nutrient concentrations. Bacterial secondary production and extracellular enzymatic activity markedly changed among different seasons and along the trophic gradient. Average bacterial secondary production increased from 0.61 to 2.09 µg Cl−1 hour−1 preceding a bloom, to 2.09 µg Cl−1 hour−1 during the bloom, decreasing again to 0.81 and 0.83 µg Cl−1 hour−1 in the post-bloom and summer periods, respectively (values from 0.5 m depth). Leucine-aminopeptidase activity showed more consistent trends than α-glucosidase activity. Average values of leucine-aminopeptidase activity, measured by enzymatic release of MCA, increased from a pre-bloom value of 164.0 to 1,712.0 (nM MCA) hour−1 released during a bloom, decreasing to 298.5 and 133.7 (nM MCA) hour−1 released for the post-bloom and summer situation, respectively (values from 0.5 m depth). Average growth rates decreased during the bloom, whereas average extracellular enzymatic activity levels expressed on a cell basis increased by an average factor of 2. Along the trophic gradient, a consistent increase in bacterial secondary production could be observed in all but the summer situation (values from 0.5 m depth). Leucine-aminopeptidase activity also showed positive trends along the gradient, while α-glucosidase activity did not exhibit such a clear trend. Bacterial biomass trends were less obvious considering both seasonal changes and the tropic gradient. Highly significant interrelations were detected between bacterial proteolytic activity, secondary production, chlorophyll a content, and nitrate concentrations, especially in the surface horizon.

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References

  1. 1.
    Burney CM, Sieburth JMcN (1977) Dissolved carbohydrates in seawater. II. A spectrophotometric procedure for total carbohydrate analysis and polysaccharide estimation. Mar Chem 5:15–28Google Scholar
  2. 2.
    Chin-Leo G, Kirchman DL (1990) Unbalanced growth in natural assemblages of marine bacterio-plankton. Mar Ecol Prog Ser 63:1–8Google Scholar
  3. 3.
    Cho BC, Azam F (1988) Heterotrophic bacterioplankton production measurement by the tritiated thymidine incorporation method. Arch Hydrobiol Beih 31:153–162Google Scholar
  4. 4.
    Cho BC, Azam F (1990) Biogeochemical significance of bacterial biomass in the ocean's euphotic zone. Mar Ecol Prog Ser 63:253–259Google Scholar
  5. 5.
    Chrost RJ, Munster U, Rai H, Albrecht D, Witzel PK, Overbeck J (1989) Photosynthetic production and exoenzymatic degradation of organic matter in the euphotic zone of a eutrophic lake. J Plank Res 11:223–242Google Scholar
  6. 6.
    Degobbis D, Gilmartin M, Revelante N (1986) An annotated nitrogen budget calculation for the Northern Adriatic Sea. Mar Chem 20:159–177Google Scholar
  7. 7.
    Degobbis D, Smodlaka N, Skrivanic I, Precali R (1979) Increased eutrophication of the Northern Adriatic Sea. Mar Pollut Bull 10:298–301Google Scholar
  8. 8.
    Fuhrman JA, Azam F (1982) Thymidine incorporation as a measure of heterotrophic bacterio-plankton production in marine surface waters: Evaluation and field results. Mar Biol 66:109–120Google Scholar
  9. 9.
    Furhman JA, Eppley RW, Hagström A, Azam F (1985) Diel variations in bacterioplankton, phytoplankton, and related parameters in the Southern California Bight. Mar Ecol Prog Ser 27:9–20Google Scholar
  10. 10.
    Fuhrman JA, Ferguson RL (1986) Nanomolar concentrations and rapid turnover of dissolved free amino acids in seawater: Agreement between chemical and microbiological measurements. Mar Ecol Prog Ser 33:237–242Google Scholar
  11. 11.
    Fuhrman JA (1987) Close coupling between release and uptake of dissolved free amino acids in seawater studied by an isotope dilution approach. Mar Ecol Prog Ser 37:45–52Google Scholar
  12. 12.
    Fuhrman JA, Sleeter TD, Carlson CA, Proctor LM (1989) Dominance of bacterial biomass in the Sargasso Sea and its ecological implications. Mar Ecol Prog Ser 57:207–217Google Scholar
  13. 13.
    Gilmartin M, Revelante N (1980) Nutrient input and the summer nanoplankton bloom in the Northern Adriatic Sea. PSZNI: Mar Ecol 1:169–180Google Scholar
  14. 14.
    Gilmartin M, Revelante N (1983) The phytoplankton of the Adriatic Sea: Standing crop and primary production. Thalassia Jugosl 19:173–188Google Scholar
  15. 15.
    Halemejko GZ, Chrost RJ (1986) Enzymatic hydrolysis of proteinaceous particulate and dissolved material in an eutrophic lake. Arch Hydrobiol 107:1–21Google Scholar
  16. 16.
    Herndl GJ, Karner M, Peduzzi P (in press) (1992) Floating mucilage in the Northern Adriatic Sea: The potential of a microbial ecological approach to solve the “mystery.” Sci Total EnvironGoogle Scholar
  17. 17.
    Herndl GJ (1991) Microbial biomass dynamics along a trophic gradient at the Atlantic Barrier Reef off Belize (Central America). PSZNI: Mar Ecol 12:41–51Google Scholar
  18. 18.
    Hobbie JE, Daley RJ, Jasper S (1977) Use of Nucleopore filters for counting bacteria by epifluorescence microscopy. Appl Environ Microbiol 33:1225–1228Google Scholar
  19. 19.
    Hollibaugh JT, Azam F (1983) Microbial degradation of dissolved proteins in seawater. Limnol Oceanogr 28:1104–1116Google Scholar
  20. 20.
    Hoppe H (1983) Significance of exoenzymatic activities in the ecology of brackish water: Measurements by means of methylumbelliferyl-substrates. Mar Ecol Prog Ser 11:299–308Google Scholar
  21. 21.
    Hoppe H, Kim S, Gocke K (1988) Microbial decomposition in aquatic environments: Combined process of extracellular enzyme activity and substrate uptake. Appl Environ Microbiol 54:784–790Google Scholar
  22. 22.
    Ittekkot V, Brockmann U, Michaelis W, Degens ET (1981). Dissolved free and combined carbohydrates during a phytoplankton bloom in the Northern North Sea. Mar Ecol Prog Ser 4:299–305Google Scholar
  23. 23.
    Ittekkot V, Degens ET, Brockmann U (1982) Monosaccharide composition of acid-hydrolyzable carbohydrates in particulate matter during a plankton bloom. Limnol Oceanogr 27:770–776Google Scholar
  24. 24.
    Johnson KM, Sieburth JMcN (1977) Dissolved carbohydrates in seawater. I. A precise spectrophotometric analysis for monosaccharides. Mar Chem 5:1–13Google Scholar
  25. 25.
    Kirchman DL, Hodson RE (1986) Metabolic regulation of amino acid uptake in marine waters. Limnol Oceanogr 31:339–350Google Scholar
  26. 26.
    Kirchman DL, Keil RG, Wheeler PA (1990) Carbon limitation of ammonium uptake by heterotrophic bacteria in the subarctic Pacific. Limnol Oceanogr 35:1258–1266Google Scholar
  27. 27.
    Lancelot C, Billen G (1984) Activity of heterotrophic bacteria and its coupling to primary production during the spring phytoplankton bloom in the southern bight of the North Sea. Limnol Oceanogr 29:721–730Google Scholar
  28. 28.
    Lee S, Fuhrman JA (1987) Relationships between biovolume and biomass of naturally derived marine bacterioplankton. Appl Environ Microbiol 53:1298–1303Google Scholar
  29. 29.
    Mopper K, Dawson R, Liebezeit G, Ittekkot V (1980) The monosaccharide spectra of natural waters. Mar Chem 10:55–66Google Scholar
  30. 30.
    Münster U, Chrost RJ (1990) Origin, composition and microbial utilization of dissolved organic matter. In: Overbeck J, Cbrost RJ (eds) Aquatic microbial ecology. Biochemical and molecular approaches. Springer, New York, pp 8–46Google Scholar
  31. 31.
    Osservatorio dell'Alto Adriatico (1991) Joint Cruise Report of the Alpen-Adria Cruises 1990Google Scholar
  32. 32.
    Parsons T, Maita Y, Lalli C (1984) A manual of chemical and biological methods for seawater analysis. Pergamon Press, New York, pp 173Google Scholar
  33. 33.
    Rosenberg R, Dahl E, Edler L, Fryberg L, Graneli E, Graneli W, Hagström Å, Lindahl O, Matos MO, Pettersson K, Sahlsten E, Tiselius P, Turk V, Wikner J (1990) Pelagic nutrient and energy transfer during spring in the open and coastal Skagerrak. Mar Ecol Prog Ser 61:215–231Google Scholar
  34. 34.
    Simon M (1985) Specific uptake of amino acids by attached and free-living bacteria in a mesotrophic lake. Appl Environ Microbiol 49:1254–1259Google Scholar
  35. 35.
    Simon M, Tizler MM (1987) Bacterial response to seasonal changes in primary production and phytoplankton biomass in Lake Constance. J Plank Res 9:535–552Google Scholar
  36. 36.
    Smodlaka N, Revelante N (1984) The influence of the Po River on the primary production of the Northern Adriatic with comments on the importance of the nanoplankton. Rapp Comm Int Mer Medit 29:97–98Google Scholar
  37. 37.
    Strickland JDH, Parsons TR (1972) A practical handbook of seawater analysis. Bulletin 167 of the Fish Res Bd CanadaGoogle Scholar
  38. 38.
    Torréton J, Guiral D, Arfi R (1989) Bacterioplankton biomass and production during destratification in a monomictic eutrophic bay of a tropical lagoon. Mar Ecol Prog Ser 57:53–67Google Scholar
  39. 39.
    Utermöhl H (1958) Zur Vervollkommnung der quantitativen Phytoplankton—Methodik. Mitt int Verein theor angewLimnol 9:1–38Google Scholar
  40. 40.
    Vives Rego J, Billen G, Fontigny A, Somville M (1985) Free and attached proteolytic activity in water environments. Mar Ecol Prog Ser 21:245–249Google Scholar
  41. 41.
    Weisse T, Müller H, Pinto-Coelho RM, Schweizer A, Springmann D, Baldringer G (1990) Response of the microbial loop to the phytoplankton spring bloom in a large prealpine lake. Limnol Oceanogr 35:781–794Google Scholar
  42. 42.
    Wing MR, Stromvall EJ, Lieberman SH (1990) Real-time determination of dissolved free amino acids and primary amines in seawater by time-resolved fluorescence. Mar Chem 29:325–338Google Scholar

Copyright information

© Springer-Verlag 1992

Authors and Affiliations

  • Markus Karner
    • 1
  • Dragica Fuks
    • 2
  • Gerhard J. Herndl
    • 1
  1. 1.Department of Marine Biology, Institute of ZoologyUniversity of ViennaViennaAustria
  2. 2.Institute Ruder BoskovicCenter for Marine ResearchYU-RovinjCroatia
  3. 3.Station ZoologiqueVillefranche-sur-merFrance

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