Applied Microbiology and Biotechnology

, Volume 43, Issue 6, pp 1028–1033 | Cite as

Oxygenation of intensive cell-culture system

  • A. N. Emery
  • D. C.-H. Jan
  • M. Al-Rueai
Original Paper

Abstract

The abilities of various methods of oxygenation to meet the demands of high-cell-density culture were investigated using a spin filter perfusion system in a bench-top bioreactor. Oxygen demand at high cell density could not be met by sparging with air inside a spin filter (oxygen transfer values in this condition were comparable with those for surface aeration). Sparging with air outside a spin filter gave adequate oxygen transfer for the support of cell concentrations above 107 ml−1 in fully aerobic conditions but the addition of antifoam to control foaming caused blockage of the spinfilter mesh. Bubble-free aeration through immersed silicone tubing with pure oxygen gave similar oxygen transfer rates to that of sparging with air but without the problems of bubble damage and fouling of the spin filter. A supra-optimal level of dissolved oxygen (478% air saturation) inhibited cell growth. However, cells could recover from this stress and reach high density after reduction of the dissolved oxygen level to 50% air saturation.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • A. N. Emery
    • 1
  • D. C.-H. Jan
    • 1
  • M. Al-Rueai
    • 1
  1. 1.BBSRC Centre for Biochemical Engineering, School of Chemical EngineeringUniversity of BirminghamEdgbaston, BirminghamUK

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