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Applied Microbiology and Biotechnology

, Volume 43, Issue 6, pp 1014–1018 | Cite as

Effects of intensity and quality of light on phycocyanin production by a marine cyanobacterium Synechococcus sp. NKBG 042902

  • H. Takano
  • T. Arai
  • M. Hirano
  • T. Matsunaga
Original Paper

Abstract

Among 150 strains, including marine cyanobacteria isolated from coastal areas of Japan and a freshwater cyanobacterium from the IAM collection, Spirulina platensis IAM M-135, the marine cyanobacterium Synechococcus sp. NKBG 042902 contained the highest amount of phycocyanin (102 mg/g dry cell weight). We have proposed that the cyanobacterium could be an alternative producer for phycocyanin. The effects of light intensity and light quality on the phycocyanin content in cells of Synechococcus sp. NKBG 042902 were investigated. When the cyanobacterium was cultured under illumination of 25 ώmol m−2 s−1 using a cool-white fluorescent lamp, the phycocyanin content was highest, and the phycocyanin and biomass productivities were 21 mg 1−1 day−1 and 100 mg 1−1 day−1 respectively. Red light was essential for phycocyanin production by this cyanobacterium. Phycocyanin and biomass production were carried out by the cyanobacterium cultures grown under only red light (peak wavelength at 660 nm) supplied from light-emitting diodes (LED). Maximum phycocyanin and biomass productivities were 24 mg 1−1 day−1 and 130 mg 1−1 day−1 when the light intensity of the LED was 55 ώmol m−2 s−1.

Keywords

Biomass Productivity Synechococcus Fluorescent Lamp Cell Weight Spirulina 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Arad S (1988) Production of biochemicals from red microalgae. Congress proceedings of Aquaculture International Congress & Exposition, September 6–9, 1988. British Columbia Pavilion Corporation, Vancouver, CanadaGoogle Scholar
  2. Babu TS, Kumar A, Varma K (1991) Effect of light quality on phycobilisome components of the cyanobacterium Spirulina plantensis. Plant Physiol 95:492–497Google Scholar
  3. Benemann JR, Tillett DM, Weissman JC (1987) Microalgae biotechnology. Trends Biotechnol 5:47–53Google Scholar
  4. Burgess JG, Iwamoto K, Miura Y, Takano H, Matsunaga T (1993) An optical fibre photobioreactor for enhanced production of the marine unicellular alga Isochrysis aff. galbana T-Iso (UTEX LB 2307) rich in docosahexaenoic acid. Appl Microbiol Biotechnol 39:456–459Google Scholar
  5. Ghosh AK (1966) Transfer of the excitation energy in Anacystis nidulans grown to obtain different pigment ratios. Biophys J 6:611–619Google Scholar
  6. Glazer AN, Stryer L (1984) Phycofluor probes. Trends Biochem Sci 9:423–427Google Scholar
  7. Goedheer JC (1976) Spectral properties of the blue-green alga Anacystis nidulans grown under different environmental conditions. Photosynthetica 10:411–422Google Scholar
  8. Jassby A (1988) Spirulina: model for microalgae as human food. In: Lambi CA, Wealand JR (eds) Algae and human affairs. Cambridge University Press, Cambridge, pp 149–179Google Scholar
  9. Jones W, Myers J (1965) Pigment variations in Anacystis nidulans induced by light of selected wavelengths. J Phycol 1:7–14Google Scholar
  10. Lonneborg A, Lind LK, Kalla SR, Gustafsson P, Oquist G (1985) Acclimation processes in the light-harvesting system of the cyanobacterium Anacystis nidulans following a light shift from white to red light. Plant Physiol 78: 110–114Google Scholar
  11. Matsunaga T (1992) Perspectives in marine biotechnology. BIOJAPAN '92, Yokohama, Japan. Japan Bioindustry AssociationGoogle Scholar
  12. Matsunaga T, Nakamura N, Tsuzaki N, Takeda H (1988) Selective production of glutamate by an immobilized marine blue-green alga, Synechococcus sp. Appl Microbiol Biotechnol 28:373–376Google Scholar
  13. Matsunaga T, Takeyama H, Nakamura N (1990) Characterization of cryptic plasmids from marine cyanobacteria and construction of a hybrid plasmid potentially capable of transformation of marine cyanobacterium, Synechococcus sp., and its transformation. Appl Biochem Biotechnol 24/25:151–160Google Scholar
  14. Matsunaga T, Takeyama H, Sudo H, Oyama N, Ariura S, Takano H, Hirano M, Burgess JG, Sode K, Nakamura N (1991) Glutamate production from CO2 by marine cyanobacterium Synechococcus sp. using a novel biosolar reactor employing light-diffusing optical fibers. Appl Biochem Biotechnol 28/29:157–167Google Scholar
  15. Mastunaga T, Burgess JG, Yamada N, Komatsu K, Yoshida S, Wachi Y (1993) An ultraviolet (UV-A) absorbing biopterin glucoside from the marine planktonic cyanobacterium Oscillatoria sp. Appl Microbiol Biotechnol 39:250–253Google Scholar
  16. Miura Y, Sode K, Nakamura N, Matsunaga N, Matsunaga T (1993) Production of λ-linolenic acid from the marine green alga Chorella sp. NKBG 042401. FEMS Microbiol Lett 107:163–168Google Scholar
  17. Myers J, Kratz WA (1955) Relations between pigment content and photosynthetic characteristics in blue-green alga. J Gen Physiol 39:11–22Google Scholar
  18. Noue JdL, Pauw Nd (1988) The potential of microalgal biotechnology: a review of production and uses of microalgae. Biotechnol Adv 6:725–770Google Scholar
  19. Ogawa T, Terui G (1970) Studies on the growth of Spirulina platensis: on the pure culture of Spirulina platensis. J Ferment Technol 48:361–367Google Scholar
  20. Oquist G (1974) Distribution of chlorophyll between the two photoreactions in photosynthesis of the blue-green alga Anacystis nidulans grown at two different light intensities. Plant Physiol 30:38–44Google Scholar
  21. Pirt SJ (1984) Algal photosynthesis: the Aladdin's cave of biotechnology. Chem Ind 3 December: 843–849Google Scholar
  22. Rippka R, Deruelles J, Waterbury JB, Herdman M, Stanier RY (1979) Genetic assignments, strain histories and properties of pure cultures of cyanobacteria. J Gen Microbiol 111:1–61Google Scholar
  23. Sode K, Horikoshi K, Takeyama H, Nakamura N, Matsunaga T (1991) On-line monitoring of marine cyanobacterial cultivation based on phycocyanin fluorescence. J Biotechnol 21:209–218Google Scholar
  24. Takano H, Takeyama H, Nakamura N, Sode K, Burgess JG, Manabe E, Hirano M, Matsunaga T (1992) CO2 removal by high density culture of a marine cyanobacterium Synechococcus sp. using an improved photobioreacter employing light-diffusing optical fibers. Appl Biochem Biotechnol 34/35:449–458Google Scholar
  25. Takano H, Furu-une H, Burgess JG, Manabe E, Hirano M, Okazaki M, Matsunaga T (1993) Production of ultra-fine calcite particles by Coccolithophorid algae grown in a biosolar reactor supplied with sunlight. Appl Biochem Biotechnol 39/40:159–167Google Scholar
  26. Venkataraman LV (1989) Spirulina state of art and emerging prospects. Phykos 28:231–250Google Scholar
  27. Wake H, Umetsu H, Shimomura K, Matsunaga T (1991) Extracts of marine cyanobacteria stimulated somatic embryogenesis of Daucus carota L. Plant Cell Rep 9:655–658Google Scholar
  28. Wake H, Akasaka A, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1992a) Enhanced germination of artificial seeds by marine cyanobacterial extract. Appl Microbiol Biotechnol 36:684–688Google Scholar
  29. Wake H, Akasaka A, Umetsu H, Ozeki Y, Shimomura K, Matsunaga T (1992b) Promotion of plantlet formation from somatic embryos of carrot treated with a high molecular weight extract from a marine cyanobacterium. Plant Cell Rep 11:62–65Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • H. Takano
    • 1
  • T. Arai
    • 2
  • M. Hirano
    • 2
  • T. Matsunaga
    • 1
  1. 1.Department of BiotechnologyTokyo University of Agriculture and TechnologyTokyoJapan
  2. 2.Department of Applied ChemistryKogakuin UniversityTokyoJapan

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