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Applied Microbiology and Biotechnology

, Volume 43, Issue 6, pp 985–988 | Cite as

Microbial production of specifically ring-13C-labelled 4-hydroxybenzoic acid

  • R. Müller
  • A. Wagener
  • K. Schmidt
  • E. Leistner
Original Paper

Abstract

When transformed with a recombinant vector carrying the ubiC gene (encoding chorismate pyruvate-lyase, EC 4.1.3.27) the triple mutant (Phe, Trp, Tyr) Klebsiella pneumoniae 62-1 excretes 4-hydroxybenzoic acid instead of chorismic acid. The recombinant strain can be used to produce in high yield specifically ring-labelled 4-hydroxybenzoic acid from isotopically labelled glucose.

Keywords

Glucose Klebsiella Recombinant Strain Klebsiella Pneumoniae Microbial Production 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • R. Müller
    • 1
  • A. Wagener
    • 1
  • K. Schmidt
    • 1
  • E. Leistner
    • 1
  1. 1.Institut für Pharmazeutische BiologieRheinische Friedrich-Wilhelms-Universität BonnBonnGermany

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