Applied Microbiology and Biotechnology

, Volume 41, Issue 1, pp 99–105 | Cite as

Development and application of thermo-sensitive magnetic immunomicrospheres for antibody purification

  • Akihiko Kondo
  • Hiroko Kamura
  • Ko Higashitani
Applied Microbial and Cell Physiology

Abstract

Ultrafine magnetite particles were prepared by a co-precipitation method. The poly-(styrene/N-isopropylacrylamide/methacrylic acid) latex particles containing ultrafine magnetite [magnetic P(St/NIPAM/MAA)] were prepared by two-step emulsifier-free emulsion polymerization. The minimum NaCl concentration for flocculation of these magnetic latex particles (critical flocculation concentration, CFC) decreased with increasing temperature. These temperature dependence of CFC, namely its thermo-sensitivity, originated from NIPAM. At a certain NaCl concentration, some of the magnetic latex particles showed reversible transition between flocculation and dispersion by controlling the temperature, and the thermo-flocculated magnetic latex particles were separated quickly in a magnetic field. Bovine serum albumin (BSA) was covalently immobilized onto the magnetic P(St/NIPAM/MAA) latex particles with high efficiency by the carbodiimide method. These thermo-sensitive magnetic immunomicrospheres were effective for the immunoaffinity purification of anti-BSA antibodies from antiserum.

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Copyright information

© Springer-Verlag 1994

Authors and Affiliations

  • Akihiko Kondo
    • 1
  • Hiroko Kamura
    • 1
  • Ko Higashitani
    • 1
  1. 1.Applied Chemistry DepartmentKyushu Institute of TechnologyKitakyushuJapan
  2. 2.Chemical Engineering DepartmentKyoto UniversityKyotoJapan

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