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Microbial Ecology

, Volume 29, Issue 3, pp 231–248 | Cite as

Attached and free-living bacteria: Production and polymer hydrolysis during a diatom bloom

  • M. Middelboe
  • M. Søndergaard
  • Y. Letarte
  • N. H. Borch
Article

Abstract

Abundance, production and extracellular enzymatic activity of free-living and attached bacteria were measured during the development and collapse of a spring bloom in a eutrophic lake. Free-living bacteria accounted for most of the total bacterial production during the first part of the bloom. Their production had a significant positive correlation to chlorophyll (P < .01) and polysaccharide concentration (P < .02) and to potential β-glucosidase and aminopeptidase activity (P < .05), suggesting that algal release of dissolved polymeric compounds provided an important carbon source for bacterial production. As the bloom collapsed, we observed a change in the activity and structure of the microbial community. The mean contribution of attached bacteria to total bacterial production increased from 12% during the first part of the bloom to 26% at the end. Also, the extracellular enzymatic activity of attached bacteria increased as the bloom collapsed and constituted up to 75% of the total hydrolytic activity. An estimated disparity between hydrolytic activity and the corresponding carbon demand of attached bacteria suggested a net release of dissolved organic compounds from organic particles via polymer hydrolysis by attached bacteria.

Keywords

Chlorophyll Hydrolytic Activity Bacterial Production Eutrophic Lake Spring Bloom 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-Verlag New York Inc 1995

Authors and Affiliations

  • M. Middelboe
    • 1
  • M. Søndergaard
    • 1
  • Y. Letarte
    • 1
  • N. H. Borch
    • 2
  1. 1.Freshwater Biological LaboratoryUniversity of CopenhagenHillerødDenmark
  2. 2.Roskilde University LibraryRoskildeDenmark

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