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Applied Microbiology and Biotechnology

, Volume 32, Issue 1, pp 45–49 | Cite as

Determination of carbohydrates in fermentation processes by high-performance liquid chromatography

  • Armin Plaga
  • Joachim Stümpfel
  • Hans-Peter Fiedler
Biotechnology

Summary

HPLC is a universal, fast, accurate and selective method for the quantification of carbohydrates during fermentation processes. HPLC is not affected by complex constituents of fermentation media, such as meat extract, soybean meal or distillers solubles. The detection limit of the different investigated carbohydrates by refractive index monitoring ranges between 20 and 40 mg/l using a cation-exchange resin and between 50 and 100 mg/l using amino- or diol-bonded phases.

Keywords

Chromatography Fermentation HPLC Carbohydrate Refractive Index 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag 1989

Authors and Affiliations

  • Armin Plaga
    • 1
  • Joachim Stümpfel
    • 1
  • Hans-Peter Fiedler
    • 1
  1. 1.LB Mikrobiologie/AntibiotikaUniversität TübingenTübingenFederal Republic of Germany

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