Advertisement

Applied Microbiology and Biotechnology

, Volume 43, Issue 4, pp 579–584 | Cite as

Metal cation uptake by yeast: a review

  • K. J. Blackwell
  • I. Singleton
  • J. M. Tobin
Mini Review

Abstract

This review addresses metal uptake specifically by yeast. Metal uptake may be passive, active or both, depending on the viability of the biomass, and is influenced by a number of environmental and experimental factors. Uptake is typically accompanied by a degree of ion exchange and, under certain conditions, may be enhanced by the addition of an energy source, Intracellularly accumulated metal is most readily associated with the cell wall and vacuole but may also be bound by other cellular organelles and biomolecules. The intrinsic biochemical, structural and genetic properties of the yeast cell along with environmental conditions are crucial for its survival when exposed to toxic metals. Conditions of pH, temperature and the presence of additional ions, amongst others, have varying effects on the metal uptake process. We conclude that yeasts have contributed significantly to our understanding of the metal uptake process and suggest directions for future work.

Keywords

Biomass Cell Wall Energy Source Yeast Cell Metal Cation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Avery SV, Tobin JM (1992) Mechanisms of strontium uptake by laboratory and bewing strains of Saccharomyces cerevisiae. Appl Environ Microbiol 58:3883–3889Google Scholar
  2. Avery SV, Tobin JM (1993) Mechanisms of adsorption of hard and soft metal ions to Saccharomyces cerevisiae and influence of hard and soft anions. Appl Environ Microbiol 59:2851–2856Google Scholar
  3. Babich H, Stotzky G (1977) Reduction in the toxicity of cadmium to microorganisms by clay minerals. Appl Environ Microbiol 33:696–705Google Scholar
  4. Baes CF, Mesner RE (1976) The hydrolysis of cations. Wiley, New YorkGoogle Scholar
  5. Belde P, Kessels B, Moelans I, Borst-Pauwels G (1988) Cd2+ uptake, Cd2+ binding and loss of cell K+ by a Cd-sensitive and a Cd-resistant strain of Saccharomyces cerevisiae. FEMS Microbiol Lett 49:493–498Google Scholar
  6. Borst-Pauwels G, Theuvenet A (1984) Apparent saturation kinetics of divalent cation uptake in yeast caused by a reduction in the surface potential. Biochim Biophys Acta 771:171–176Google Scholar
  7. Brady D, Duncan JR (1994a) Bioaccumulation of metal cations by Saccharomyces cerevisiae. Appl Microbiol Biotechnol 41:149–154Google Scholar
  8. Brady D, Duncan JR (1994b) Cation loss during accumulation of heavy metal cations by Saccharomyces cerevisiae. Biotechnol Lett 10:543–548Google Scholar
  9. Brady D, Duncan JR (1994c) Binding of heavy metals by the cell walls of Saccharomyces cerevisiae. Enzyme Microb Technol 16:633–638Google Scholar
  10. Brady D, Stoll AD, Starke L, Duncan JR (1994) Chemical and enzymatic extraction of heavy metal binding polymers from isolated cell walls of Saccharomyces cerevisiae. Biotechnol Bioeng 44:297–302Google Scholar
  11. Butt T, Ecker D (1987) Yeast metallothionein and applications in biotechnology. Microbiol Rev 51:351–364Google Scholar
  12. Cabral J (1992) Limitations f the use of an ion-selective electrode in the study of the uptake of Cu2+ by Pseudomonas syringae cells. J Microbiol Methods 16:149–156Google Scholar
  13. Davidova E, Kasparova S (1992) Adsorption of metals by yeast cell walls. Mikrobiologiya 61:1018–1022Google Scholar
  14. Ford T, Mitchell R (1992) Microbial transport of toxic metals. In: Environ Microbiol, Wiley-Liss,,New York, pp 83–101Google Scholar
  15. Fourest E, Roux JC (1992) Heavy metal biosorption by fungal mycelial by-products: mechanisms and influence of pH. Appl Microbiol Biotechnol 37:399–403Google Scholar
  16. Gadd GM (1990a) Biosorption Chem Ind 13:421–426Google Scholar
  17. Gadd GM (1990b) Heavy metal accumulation by bacteria and other microorganisms. Experientia 46:834–840Google Scholar
  18. Gadd GM (1993) Interaction of fungi with toxic metals. New Phytol 124:25–60Google Scholar
  19. Gadd GM, Mowll JL (1983) The relationship between cadmium uptake, potassium release and viability in Saccharomyces cerevisiae. FEMS Microbiol Lett 16:45–48Google Scholar
  20. Huang C, Huang P, Morehart A (1990) The removal of Cu(II) from dilute aqueous solutions by Saccharomyces cerevisiae. Water Res 24:433–439Google Scholar
  21. Hughes MN, Poole RK (1989) Metals and micro-organisms. Chapman & Hall, LondonGoogle Scholar
  22. Joho M, Tarumi K, Inouhe M, Tohoyoma H, Murayama T (1991) Co2+ and Ni2+ resistance in Saccharomyces cerevisiae associated with a reduction in the accumulation of Mg2+. Microbios 67:177–186Google Scholar
  23. Jones RP, Gadd GM (1990) Ionic nutrition of yeast-physiological mechanisms involved and implications for biotechnology. Enzyme Microb Technol 12:1–17Google Scholar
  24. Junghans K, Straube G (1991) Biosorption of copper by yeasts. Biol Metals 4:233–237Google Scholar
  25. Mowll JL, Gadd GM (1984) Cadmium uptake by Aureobasidium pullulans. J Gen Microbiol 130:279–284Google Scholar
  26. Murray AD, Kidby DK (1975) Sub-cellular location of mercury in yeast grown in the presence of mercuric chloride. J Gen Microbiol 86:66–74Google Scholar
  27. Nakajima A, Sakaguchi T (1993) Accumulation of uranium by basidiomycetes. Appl Microbiol Biotechnol 38:574–578Google Scholar
  28. Nieuwenhuis B, Weijers C, Borst-Pauwels G (1981) Uptake and accumulation of Mn2+ and Sr2+ in Saccharomyces cerevisiae. Biochim Biophys Acta 649:83–88Google Scholar
  29. Norris PR, Kelly DP (1977) Accumulation of cadmium and cobalt by Saccharomyces cerevisiae. J Gen Microbiol 99:317–324Google Scholar
  30. Okorokov L, Lichko L, Kadomtseva V, Titovsky V, Kulaev I (1977) Energy-dependent transport of manganese into yeast cells and distribution of accumulated ions. Eur J Biochem 75:373–377Google Scholar
  31. Okorokov L, Lichko L, Andreeva N (1983) Changes of ATP, polyphosphate and K+ contents in Saccharomyces carlsbergensis during uptake of Mn2+ and glucose. Biochem 6:481–488Google Scholar
  32. Ono B, Ohue H, Ishihara F (1988) Role of cell wall in Saccharomyces cerevisiae mutants resistant to Hg2+. J Bacteriol 170:5877–5882Google Scholar
  33. Perkins J, Gadd GM (1993) Accumulation and intracellular compartmentation of lithium ions in Saccharomyces cerevisiae. FEMS Microbiol Lett 107:255–260Google Scholar
  34. Remacle J (1990) The cell wall and metal binding. In: Volesky B (ed) Biosorption of heavy metals, CRC, Boca Raton, FlaGoogle Scholar
  35. Rome L de, Gadd GM (1987) Measurement of copper uptake in Saccharomyces cerevisiae using a Cu2+-selective electrode. FEMS Microbiol Lett 43:283–287Google Scholar
  36. Roomans G, Theuvenet A, Van Den Berg T, Borst-Pauwels G (1979) Kinetics of Ca2+ and Sr2+ uptake by yeast. Effects of pH, cations and phosphate. Biochim Biophys Acta 551:187–196Google Scholar
  37. Scot JA, Palmer SJ (1990) Sites of cadmium uptake in bacteria used for biosorption. Appl Microbiol Biotechnol 33:221–225Google Scholar
  38. Tobin JM, Cooper DJ, Neufeld RJ (1984) Uptake of metal ions by Rhizopus arrhizus biomass. Appl Environ Microbiol 47:821–824Google Scholar
  39. Tobin JM, Cooper DG, Neufeld RJ (1987) Influence of anions on metal adsorption by Rhizopus arrhizus biomass. Biotechnol Bioeng 30:882–886Google Scholar
  40. Volesky B (1990) Biosorption by fungal biomass. In: Volesky B (ed) Biosorption of heavy metals. CRC, Boca Raton, FlaGoogle Scholar
  41. Volesky B, May H, Holan ZR (1993) Cadmium biosorption by Saccharomyces cerevisiae. Biotechnol Bioeng 41:826–829Google Scholar
  42. Wang H, Wood JM (1984) Bioaccumulation of nickel by algae. Environ Sci Technol 18:106–109Google Scholar
  43. White C, Gadd GM (1986) Uptake and cellular distribution of copper, cobalt and cadmium in strains of Saccharomyces cerevisiae cultured on elevated concentrations of these metals. FEMS Microbiol Ecol 38:277–283Google Scholar
  44. White C, Gadd GM (1987) The uptake and cellular distribution of zinc in Saccharomyces cerevisiae. J Gen Microbiol 133:727–737Google Scholar
  45. Wood JM, Wang H (1983) Microbial resistance to heavy metals. Environ Sci Technol 12:582–590Google Scholar

Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • K. J. Blackwell
    • 1
  • I. Singleton
    • 2
  • J. M. Tobin
    • 1
  1. 1.School of Biological SciencesDublin City UniversityDublin 9Ireland
  2. 2.Department of Industrial MicrobiologyUniversity College DublinDublin 4Ireland

Personalised recommendations