Applied Microbiology and Biotechnology

, Volume 36, Issue 1, pp 136–141 | Cite as

Capacity of siderophore — producing alkalophilic bacteria to accumulate iron, gallium and aluminum

  • D. J. Gascoyne
  • J. A. Connor
  • A. T. Bull
Environmental Biotechnology

Summary

The ability of alkalophilic bacteria to remove iron, gallium and aluminium from culture media is reported. Of six bacterial strains grown in the presence of iron, gallium or aluminium (10 μM), five were able to accumulate iron or gallium, but only two depleted the aluminium stock. A comparison of gallium removal under low (< 1 gmM) or high (10 gmM) iron conditions showed that two isolates accumulated gallium only under low-Fe conditions. One isolate, a coryneform bacterium, was able to grow in the presence of 1, 10 and 100 mg gallium/l, but growth and siderophore production were affected at high gallium concentration. Similar concentrations of gallium were accumulated from cultures initially containing 1 or 100 mg gallium/l.

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Charley RC, Bull AT (1979) Bioaccumulation of silver by a multispecies community of bacteria. Arch Microbiol 123:239–244Google Scholar
  2. Dunn GM, Bull AT (1983) Bioaccumulation of copper by a defined community of activated sludge bacteria. Eur J Appl Microbiol Biotechnol 17:30–34Google Scholar
  3. Emery T (1971) Role of ferrichrome as a ferric ionophore in Ustilago sphaerogena. Biochemistry 10:1483–1488Google Scholar
  4. Gascoyne DJ, Connor JA Bull AT (1991) Isolation of bacteria producing siderophores under alkaline conditions. Appl Microbiol Biotechnol 36:130–135Google Scholar
  5. Grant WD, Mwatha WE, Jones BE (1990) Alkaliphiles: ecology, diversity and applications. FEMS Microbiol Rev 75:255–270Google Scholar
  6. Hantke K (1982) Negative control of iron uptake systems in Escherichia coli. FEMS Microbiol Lett 15:83–86Google Scholar
  7. Hubbard JAM, Lewandowska KB, Hughes MN, Poole RK (1986) Effects of iron-limitation of Escherichia coli on growth, the respiratory chains and gallium uptake. Arch Microbiol 146:80–86Google Scholar
  8. Krulwich TA, Guffanti AA (1989) Alkalophilic bacteria. Annu Rev Microbiol 43:435–463Google Scholar
  9. Krulwich TA, Guffanti AA, Seto-Young D (1990) pH homeostasis and bioenergetic work in alkalophiles. FEMS Microbiol Rev 75:271–278Google Scholar
  10. Leong J, Neilands JB, Raymond KN (1974) Coordination isomers of biological iron transport compounds III. (1) Transport of γ-cis-chromic desferriferrichrome by Ustilago sphaerogena. Biochem Biophys Res Commun 60:1066–1071Google Scholar
  11. Pesic B, Zhou T (1988) Recovering gallium with Kelex 100. K Metals 24–26Google Scholar
  12. Pooley FD (1982) Bacteria accumulate silver during leaching of sulphide ore minerals. Nature 296:642–643Google Scholar
  13. Russell LM, Holmes RK (1983) Initial characteristtion of the ferric iron transport system of Corynebacterium diphtheriae. J Bacteriol 155:1439–1442Google Scholar
  14. Schwyn B, Neilands JB (1987) Universal chemical assay for the detection and determination of siderophores. Anal Biochem 160:47–56Google Scholar
  15. Shumate SE, Strandberg GW (1985) Accumulation of metals by microbial cells. In: Robinson CW, Howell JA (eds) Comprehensive biotechnology, vol 4. Pergamon Press, Oxford, pp 235–347Google Scholar
  16. Tonge MD (1989) The aqueous and analytical chemistry of aluminium in relation to renal dialysis. Ph. D. thesis, University of ManchesterGoogle Scholar
  17. Waring WS, Werkman CH (1942) Growth of bacteria in an iron-free medium. Arch Biochem 1:303–310Google Scholar
  18. Winkelmann G (1986) Iron complex products (siderophores). In: Pape H, Rehm H-J (eds) Biotechnology, vol 4, Microbial Products II. VCH, Weinheim, pp 215–243Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • D. J. Gascoyne
    • 1
  • J. A. Connor
    • 2
  • A. T. Bull
    • 1
  1. 1.Biological LaboratoryUniversity of KentKentUK
  2. 2.Chemical LaboratoryUniversity of KentKentUK

Personalised recommendations