Applied Microbiology and Biotechnology

, Volume 36, Issue 1, pp 1–4 | Cite as

On the safety of Bacillus subtilis and B. amyloliquefaciens: a review

  • Anne de Boer Sietske
  • Børge Diderichsen


Bacillus Bacillus Subtilis 
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  1. AMFEP (Association of Microbial Food Enzyme Producers) (1990) Regulatory aspects of food enzymes produced by recombinant microorgganisms. Association of Microbial Food Enzyme Producers, BruxellesGoogle Scholar
  2. Andersen JR, Diderichsen BK, Hjortkjaer RK, Boer AS de, Bootman J, West H, Ashby R (1987) Determining the safety of maltogenic amylase produced by rDNA technology. J Food Protect 50:521–526Google Scholar
  3. Bielecki J, Youngmans P, Connelly P, Portnoy DA (1990) Bacillus subtilis expressing a haemolysin gene from Listeria monocytogenes can grow in mammalian cells. Nature 345:175–176Google Scholar
  4. Burkholder PR, Giles NH (1947) Induced biochemical mutants in Bacillus subtilis. Am J Bot 34:345–348Google Scholar
  5. Cotton DJ, Gill VJ, Marshall DJ, Gress J, Thaler M, Pizzo PA (1987) Clinical features and therapeutic interventions in 17 cases of Bacillus bacteriemia in an immunosuppressed patient population. J Clin Microbiol 25:672–674Google Scholar
  6. CPC International (1986) GRAS Petition 4G0293 proposing that alpha-amylase from a strain of Bacillus subtilis (ATCC 39 705) containing the gene for alpha-amylase from B. stearothermophilus inserted by recombinant DNA techniques be affirmed as GRAS as a direct human food ingredient. Notice of Filing. Federal Register 51:10571Google Scholar
  7. Diderichsen B et al. (1990) The conditions for contained use of genetically engineered microorganisms and cells for industrial production in Europe. Biotechnol Forum Eur 7:484–485Google Scholar
  8. Djien KS, Hesseltine CW (1979) Tempe and related foods. In: Rose AH (ed) Economic microbiology, vol 4. Academic Press, London, pp 116–140Google Scholar
  9. Donzis PB, mondino BJ, Wiessman BA (1988) Bacillus keratitis associated with contaminated contact lens care systems. Am J Ophthalmol 105:195–197Google Scholar
  10. Doyle RJ, Keller KF, Ezzell JW (1985) In: Ballows A, Hausler WJ, Shadomy HJ (eds) Manual of clinical microbiology, 4th edn. American Society for Microbiology, Washington, D. C., pp 211–215Google Scholar
  11. Ehrlich SD (1977) Replication and expression of plasmids from Staphylococcus aureus in Bacillus subtilis. Proc Natl Acad Sci USA 74:1680–1682Google Scholar
  12. Enzyme Bio-Systems (1988) GRAS Petition 7G0328 proposing that alpha-amylase from a strain of Bacillus subtilis containing a Bacillus megaterium alpha-amylase gene be affirmed as GRAS as a direct human food ingredient. Notice of Filing. Federal Register 53:16191Google Scholar
  13. Finch JE, Prince J, Hawksworth M (1978) A bacteriological survey of the domestic environment. J Appl Bacteriol 45:364–375Google Scholar
  14. Gordon RE, Haynes WC, Pang CH-N (1973) The genus Bacillus. In: Gordon RE, Haynes WC, Pang CH-N (eds) Agricultural Handbook no. 427. US Department of Agriculture, Washington, D. C., pp 23–25Google Scholar
  15. IFBC (International Food Biotechnology Council) (1990) Biotechnologies and food: assuring the safety of foods produced by genetic modification. Regul Toxicol Pharmacol 12:Special IssueGoogle Scholar
  16. Ihde DC, Armstrong D (1973) Clinical spectrum of infection due to Bacillus species. Am J Med 55:839–845Google Scholar
  17. Jonas M, Cunha BA, Greensher J (1981) Bacillus subtilis osteomycelitis. South Med J 74:1421–1422Google Scholar
  18. Kiss T, Gratwohl A, Frei R, Osterwalder B, Tichelli A, Speck B (1988) Bacillus subtilis Infectionen. Schweiz Rundsch Med 77:1219–1223Google Scholar
  19. Kramer JM, Gilbert RJ (1989) Bacillus cereus and other Bacillus species. In: Doyle MP (ed) Foodborne bacterial pathogens. Dekker, New York, pp 21–70Google Scholar
  20. Logan NA (1988) Bacillus species of medical and veterinary importance. J Med Microbiol 25:157–165Google Scholar
  21. National Academy of Sciences (1987) Introduction of recombinant DNA-engineered organisms into the environment: key issues. National Academy Press, Washington, DCGoogle Scholar
  22. Novo Laboratories (1990) GRAS Petition 7G0326 proposing that maltogenic amylase enzyme preparation derived from a genetically modified Bacillus subtilis be affirmed as GRAS as a direct human food ingredient. Federal Register 55:9772–9773Google Scholar
  23. OECD (1986) Recombinant DNA safety considerations. ParisGoogle Scholar
  24. Pariza MW, Foster EM (1983) Determining the safety of enzymes used in food processing. J Food Prot 46:453–468Google Scholar
  25. Pennington JE, Gibbons ND, Strobeck JE, Simpson GL, Myerowitz RL (1976) Bacillus species infections in patients with haematologic infections. J Am Med Assoc 235:1473–1474Google Scholar
  26. Priest FG (1990) Products and applications. In: Harwood CR (ed) Bacillus. Plenum, New York, pp 293–320Google Scholar
  27. Priest FG, Goodfellow M, Shute LA, Berkeley RCW (1987) Bacillus amyloliquefaciens sp. nov., nom. rev. Int J Syst Bacteriol 37:69–71Google Scholar
  28. Reller LB (1973) Endocarditis caused by Bacillus subtilis. Am J Clin Pathol 60:714–718Google Scholar
  29. Richard D, Auwera P van der, Snoeck R, Daneau D, Meunier F (1988) Nosocomial bacteremia caused by Bacillus species. Clin Microbiol Infect Dis 7:783–785Google Scholar
  30. Shamsuddin D, Tuason CU, Levy C, Curtin J (1982) Bacillus cereus phanophthalmitis: source of the organism. Rev Infect Dis 4:97–103Google Scholar
  31. Tuazon CV, Murray HW, Levy C, Solny MN, Curtin JA, Sheagrren JN (1979) Serious infections from Bacillus sp. J Am Med Assoc 241:1137–1140Google Scholar
  32. Welker NE, Campbell LL (1967) Unrelatedness of Bacillus amyloliquefaciens and Bacillus subtilis. J Bacteriol 94:1124–1130Google Scholar
  33. Weller PF, Nicholson A (1979) The spectrum of Bacillus bacteremias in heroin addicts. Arch Internal Med 139:293–294Google Scholar

Copyright information

© Springer-Verlag 1991

Authors and Affiliations

  • Anne de Boer Sietske
    • 1
  • Børge Diderichsen
    • 1
  1. 1.Novo Nordisk A/SBagsvaerdDenmark

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