Applied Microbiology and Biotechnology

, Volume 44, Issue 1–2, pp 225–229 | Cite as

The ability of an environmental isolate of Pseudomonas fluorescens to utilize chrysene and other four-ring polynuclear aromatic hydrocarbons

  • G. Caldini
  • G. Cenci
  • R. Manenti
  • G. Morozzi
Environmental Biotechnology


A Pseudomonas fluorescens strain isolated from exhausted-oil-polluted soil was selected for its ability to grow on and degrade chrysene as a sole carbon source. The same strain was able to grow on and degrade benz[a]anthracene, benzo[b]naphthothiophene, but not benz[a]acridine. After 2 days of incubation on a mineral medium supplemented with chrysene at 1 μg/ml, reached by adding the Polynuclear aromatic hydrocarbon dissolved in a water-miscible solvent to the medium, the cell number had increased by 102–103. The biodegradation rate followed first-order kinetics and at its maximum value was independent of substrate concentration, as happens when the substrates are solubilized.


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Copyright information

© Springer-Verlag 1995

Authors and Affiliations

  • G. Caldini
    • 1
  • G. Cenci
    • 1
  • R. Manenti
    • 1
  • G. Morozzi
    • 1
  1. 1.Dipartimento di Biologia Cellulare e Molecolare, Sezione Science Igienistiche, Microbiologiche e AmbientaliUniversitá di PerugiaPerugiaItaly

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